DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid

A technology of mesenchymal stem cells and injection, applied in the field of cell biology, can solve the problems of serum culture affecting mesenchymal stem cells inducing differentiation function, increasing the risk of exogenous protein rejection, increasing the risk, etc., and achieving good differentiation ability, reducing the risk of contamination, ensuring the effect of differentiation ability

Pending Publication Date: 2021-05-11
朱灏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the certain toxic effect of dimethyl sulfoxide, the freezing liquid with high content of dimethyl sulfoxide increases the risk of clinical use
In addition, the introduction of exogenous animal serum may increase the risk of rejection caused by exogenous proteins in clinical research and application. At the same time, due to the uncertainty of serum components and the instability of serum culture, it may affect the normal induction and differentiation of mesenchymal stem cells. Function

Method used

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  • DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid
  • DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid
  • DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid

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preparation example Construction

[0038]The second aspect of the present invention provides a method for preparing a DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid, comprising the following steps:

[0039] S1. According to the formula, add dextran 40 glucose injection to a 500mL receiver, then slowly add compound electrolyte injection, sodium chloride injection, glucose injection, mesenchymal stem cell serum-free medium, Shake sideways until mixed;

[0040] S2. Then slowly add the formulated amount of human serum albumin, and mix upside down to obtain the DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation solution.

[0041] When the preparation raw material also includes a serum substitute, the preparation method includes the following steps:

[0042] S1. According to the formula, add dextran 40 glucose injection to a 500mL receiver, and then slowly add compound electrolyte injection, sodium chloride injection, glucose injection, mesenchymal...

Embodiment 1

[0055] Example 1 provides a DMSO-free human umbilical cord mesenchymal stem cell injection frozen storage solution, in parts by volume, its preparation raw materials include 55 parts of compound electrolyte injection, 30 parts of dextran 40 glucose injection, sodium chloride 5 parts of injection, 5 parts of glucose injection, 40 parts of human serum albumin; 6 parts of serum-free medium for mesenchymal stem cells.

[0056] The compound electrolyte injection was purchased from Hebei Tiancheng Pharmaceutical Co., Ltd.;

[0057] The dextran 40 glucose injection was purchased from Sichuan Kelun Pharmaceutical Co., Ltd.;

[0058] The sodium chloride injection was purchased from Guangdong Otsuka Pharmaceutical Co., Ltd.;

[0059] The glucose injection was purchased from Jimin Health Management Co., Ltd.;

[0060] The human albumin was purchased from Octapharma AB;

[0061] The serum-free medium for mesenchymal stem cells was purchased from Wuhan Punuosai Life Science and Technolo...

Embodiment 2

[0070] Example 2 provides a DMSO-free human umbilical cord mesenchymal stem cell injection frozen storage solution, in parts by volume, its preparation raw materials include 55 parts of compound electrolyte injection, 30 parts of dextran 40 glucose injection, sodium chloride 5 parts of injection, 5 parts of glucose injection, 40 parts of human serum albumin; 6 parts of serum-free medium for mesenchymal stem cells, 2 parts of serum substitute.

[0071] The compound electrolyte injection was purchased from Hebei Tiancheng Pharmaceutical Co., Ltd.;

[0072] The dextran 40 glucose injection was purchased from Sichuan Kelun Pharmaceutical Co., Ltd.;

[0073] The sodium chloride injection was purchased from Guangdong Otsuka Pharmaceutical Co., Ltd.;

[0074] The glucose injection was purchased from Jimin Health Management Co., Ltd.;

[0075] The human albumin was purchased from Octapharma AB;

[0076] The serum-free medium for mesenchymal stem cells was purchased from Wuhan Punuo...

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Abstract

The invention discloses a DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid, which is characterized by being prepared from the following raw materials in parts by volume: 50 to 60 parts of compound electrolyte injection, 20 to 40 parts of dextran 40 glucose injection, 1 to 10 parts of sodium chloride injection, 1 to 10 parts of glucose injection, 30 to 50 parts of human serum albumin, and 1 to 10 parts of a mesenchymal stem cell serum-free medium. The cryopreservation liquid does not contain DMOS or serum, so that the risk of clinical use is reduced, the influence of the uncertainty of serum components and the instability of serum culture on the normal induced differentiation function of the mesenchymal stem cells is avoided, and the cryopreservation liquid enables the human umbilical cord mesenchymal stem cells to keep a good cryopreservation effect, and the human umbilical cord mesenchymal stem cells have high survival rate after cryopreservation and resuscitation. In addition, the cells cryopreserved by the cryopreservation liquid can be directly diluted and then applied clinically, components of the cryopreservation liquid do not need to be removed through centrifugation, and the cryopreservation liquid can be used as an auxiliary material and directly applied to clinical administration, so that the cryopreservation liquid is more convenient to use.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to a DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation solution. Background technique [0002] Mesenchymal stem cells are multipotent cells with self-renewal ability, which were first discovered in bone marrow and can differentiate into bone, cartilage, fat, muscle and other tissues. Mesenchymal stem cells can now be isolated not only from bone marrow, but also from tissues such as fat, synovium, umbilical cord, and placenta. Compared with bone marrow, umbilical cord has a wide range of sources, is easy to obtain, has a low virus contamination rate (due to the existence of the fetal blood barrier), does not cause trauma to the donor, and has no ethical and moral restrictions. Therefore, human umbilical cord mesenchymal stem cells have a more ideal development. prospect. [0003] The most commonly used technique for cryopreservation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226A01N1/0284
Inventor 朱灏
Owner 朱灏
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