42results about How to "Maintain biological function" patented technology

The invention discloses a DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid, which is characterized by being prepared from the following raw materials in parts by volume: 50 to 60 parts of compound electrolyte injection, 20 to 40 parts of dextran 40 glucose injection, 1 to 10 parts of sodium chloride injection, 1 to 10 parts of glucose injection, 30 to 50 parts of human serum albumin, and 1 to 10 parts of a mesenchymal stem cell serum-free medium. The cryopreservation liquid does not contain DMOS or serum, so that the risk of clinical use is reduced, the influence of the uncertainty of serum components and the instability of serum culture on the normal induced differentiation function of the mesenchymal stem cells is avoided, and the cryopreservation liquid enables the human umbilical cord mesenchymal stem cells to keep a good cryopreservation effect, and the human umbilical cord mesenchymal stem cells have high survival rate after cryopreservation and resuscitation. In addition, the cells cryopreserved by the cryopreservation liquid can be directly diluted and then applied clinically, components of the cryopreservation liquid do not need to be removed through centrifugation, and the cryopreservation liquid can be used as an auxiliary material and directly applied to clinical administration, so that the cryopreservation liquid is more convenient to use.

The invention belongs to the technical field of biomaterials and particularly relates to refined manufacturing of a photo-control-hybridized cross-linked degradable support and bone tissue engineeringapplication of the photo-control-hybridized cross-linked degradable support. A method for preparing the photo-control-hybridized cross-linked degradable support comprises the steps: 1) preparing modified hydroxyapatite; 2) preparing a bonder GelMA; and 3) blending a blue-light initiator and a thickener to prepare slurry, subjecting double bonds in the GelMA and double bonds of silane-coupler-modified grafted hydroxyapatite to an interaction, i.e., a formula shown in the description in the presence of free radicals through 3D printing technique forming under the condition of illumination of specific wavelength, then, initiating monomer polymerization, and carrying out bonding, thereby forming the solid-phase hybridized degradable support. The invention relates to application of the osteogenesis-remediation-promoting photo-crosslinked composite 3D-printed hybridized degradable support as a drug carrier or / and a bioscaffold material. The support is scientific in design and simple and convenient in operation and can be applied to carrier support materials of bone-defected regenerated remediation or all classes of bioactive substances due to refined controllable processing characteristics and biodegradable characteristics.

The invention discloses a ultra-low temperature conserving solution of animal cell and conserving method thereof for conserving cell especially fresh separated primary animal cell, e.g. liver cell. Culturing fresh separated animal cell at 37 DEG C 0-24 h, changing culture medium as ultra-low temperature conserving solution at 0-4 DEG C then conserving 0-48 months at -80--196 DEG C. After conserving finished, recoverying culturing in a fresh cell culture medium at 37 DEG C, finally culturing in a normal animal cell culture medium. Adding Chinese medicine effective constituent and other chemical protective agent in conserving process and before and after culture medium to improve survival rate of animal cell after conserving at ultra-low temperature and function of cell. The ultra-low temperature conserving technique of separated animal cell is used for conveying and conserving biotype artifical organs in consist of biology artifical liver and other organ cell, also suit for application of animal cell in other medicine field.

The invention provides a low-temperature preservation and transportation culture medium. The low-temperature preservation and transportation culture medium comprises the following components: a melanin skin model culture solution, a buffer system, agarose, sucrose, glycine, adenosine, alpha-tocopherol, vitamin C, sphingosine-1-phosphoric acid, Y-27632 and heparin, wherein the melanin skin model culture solution comprises a basic culture solution, hydrocortisone, insulin, growth factors, epinephrine, 12-myristic acid-13-phorbol acetate, stem cell factors, glutamine, calcium chloride and antibiotics.

The invention relates to the technical field of cryopreservation liquid preparation, in particular to a preparation method of a human umbilical cord mesenchymal stem cell injection cryopreservation liquid. The preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation liquid at least comprises the following steps: adding a glucose injection, a sodium chloride injection, dextran and a compound electrolyte injection into dimethyl sulfoxide, and uniformly mixing; and then adding the human serum albumin, and uniformly mixing to obtain the cryoprotectant. The preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation liquid is optimized, so that the prepared cryopreservation liquid has high stability, no floccules or precipitates are generated in the preparation process and after the preparation process is completed, the functional indexes and biological functions of human umbilical cord mesenchymal stem cells are maintained, and the safety and the biocompatibility of the human umbilical cord mesenchymal stem cell back transfusion to a human body are improved.

The invention belongs to the technical field of biology, in particular to a preparation method of full-length Fas-associated death domain (FADD) protein, which utilizes a recombinant DNA technique to express and prepare the full-length FADD protein which has good stability, high yield, the same high-level structure as a wild FADD and activity for inducing cell apoptosis, and the preparation method has more convenient and faster separation and purification process and high yield. The full-length FADD protein can be applied to prepare a medicament for inducing the cell apoptosis and treat diseases such as tumors, rheumatoid arthritis, and the like. The invention also provides an expression method for expressing the full-length FADD protein in a prokaryotic expression system, which can effectively improve the yield of the full-length FADD protein.

The invention relates to the technical field of bioengineering, and particularly relates to a method and a medium for amplifying bone-marrow mesenchymal stem cells and application. The method for amplifying bone-marrow mesenchymal stem cells comprises the steps of treating the bone-marrow mesenchymal stem cells with bFGF and VEGF to amplify the bone-marrow mesenchymal stem cells. The bFGF and VEGFare added to the medium. The bone-marrow mesenchymal stem cells cultured by the method provided by the invention can increase the proliferation ability of the cells, and maintain the biological characteristics and functions of the cells, and also can increase the expression rate of the cell surface molecule CD105, thereby being useful for transplant treatment of acute myocardial infarction, chronic myocardial infarction, and ischemic heart disease caused by cardiac failure.

The invention relates to an invitro tissue cultivation method of hepatocyte of a mammal, adopting a hepatocyte culture medium with a three-dimensional porous structure of static tissue. The medium has a diameter of 1-2mm and a thickness of 3-4mm; the medium is provided with holes differing in size, wherein the diameter of the hole ranges from 80mum-500mum, and holes are communicated with each other. The method ensures that the hepatocyte of human or animal is rapidly multiplied during culture in vitro and keeps the cell biology and function of the hepatocyte cell.

The invention discloses a magnesium-based metal material conversion film and a preparation method thereof. The conversion film is mainly composed of a polyphenol organic conversion layer on the surface of a magnesium base material. The preparation method is as follows: (1) pretreatment of the magnesium metal substrate, followed by sandpaper polishing, NaOH activation, dopamine soaking, etc.; (2) cleaning the pretreated sample with deionized water at room temperature, and immersing it in polyphenolic compound and magnesium After reacting in the mixed salt solution for 2-15 minutes, ultrasonically clean with ethanol to complete a reaction cycle; repeat the reaction cycle 3-5 times to obtain a magnesium-based metal material conversion film. The conversion film prepared by the preparation method of the present invention can effectively solve the technical problems of low structural stability and poor long-term corrosion resistance of the conversion film, and the polyphenolic compound helps to capture excess active oxygen in the body and protect the body from attacked by reactive oxygen species. The conversion coating can be applied to the surface modification research of fully degradable magnesium-based metal cardiovascular stents and fully degradable magnesium-based orthopedic filling materials.

The invention relates to a method for culturing and freezing amniotic mesenchymal stem cells. The culturing method includes amniotic membrane tissue separation, amniotic mesenchymal stem cell separation, P0 generation amniotic mesenchymal stem cell culture and expansion culture. The culture method of amniotic mesenchymal stem cells of the present invention, in the separation stage of amniotic mesenchymal stem cells, adopts a special mixed enzyme digestive solution system (final concentration of each component in the digestive solution: 1.5-2U / mL neutral protease, 0.5mg / mL deoxyribonuclease I and 1mg / mL collagenase IV) for digestion, can more effectively separate amniotic mesenchymal stem cells from amniotic tissue, and then significantly improve the yield of P0 generation cells. The present invention adopts one-step digestion Compared with the traditional two-step digestion method, the operation is more convenient, and the cultured amniotic mesenchymal stem cells have good activity, high yield, high purity, and good repeatability.

The invention discloses a T lymphocyte cryopreservation solution, which is prepared from the following raw materials by volume: 1-5 parts of DMSO, 5-15 parts of dextran 40 glucose injection, 40-60 parts of compound electrolyte injection, 20-40 parts of human serum albumin and 5-15 parts of Dulbecco's medium or Dulbecco's modified medium. The invention also discloses a preparation method and a cryopreservation method thereof, the content of DMOS is reduced, so that the clinical use risk is reduced, the T lymphocyte still keeps a good cryopreservation effect, and the T lymphocyte has high survival rate and keeps a better form after cryopreservation and resuscitation. Besides, in the preparation process, the phenomenon that the human serum albumin generates protein precipitation when encountering DMSO is also solved, cells cryopreserved by the cryopreservation solution can be directly diluted and then applied clinically, components of the cryopreservation solution do not need to be centrifugally removed, and the cryopreservation solution can be used as an auxiliary material and is directly applied to clinical administration, so that the cryopreservation solution is more convenient to use.

The invention discloses a double-layer cell co-culture device with an adjustable gap. The double-layer cell co-culture device comprises a cell culture box, a cell culture groove and a driving part, wherein the cell culture box is used for culturing a first cell; the cell culture groove is used for culturing a second cell, and is arranged in the cell culture box in a removable manner; and the driving part drives the cell culture groove to move to the bottom of the cell culture box in the cell culture box, and can adjust the gap between the bottom of the cell culture box and the bottom of the cell culture groove to the predetermined distance. According to the double-layer cell co-culture device provided by the invention, the driving part drives the cell culture groove to move to the bottom of the cell culture box, and thus, the distance between the double layers of cells is adjusted in an on-line manner, the gap can adjust the growth and the function of the cells, so that the adjustable growing environment is provided for cell co-culture, and the feasible device is provided for exploring a cell co-culture technology.

The invention discloses a low-temperature preserved solid culture medium used for a tissue engineering skin model and a preservation method of the low-temperature preserved solid culture medium. The low-temperature preserved solid culture medium is prepared by the following steps: adding a low-temperature protection agent into a common culture medium for epidermis cells to prepare a basic culture solution; and mixing the basic culture solution with agarose gel and condensing to form the solid culture medium suitable for the skin model. The tissue engineering skin model is embedded into the culture medium, is preserved at 4-8 DEG C for 24-72 hours, and then is resuscitated by a common epidermis cell culture solution. The solid culture medium can be used for reducing tissue activity reduction and structure damages of cells and skin tissues, caused by a low-temperature preservation condition, to the greatest extent.

The invention discloses a degradable and foldable biological amnion composite repair stent. The degradable and foldable biological amnion composite repair stent comprises a tube-shaped body with an axially extending through hole, an elastic balloon is arranged at the front end of the tube-shaped body, a one-way valve is connected to the tail end of the tube-shaped body, the one-way valve closes the through hole at the position, a foldable net-pipe-shaped polylactic acid bracket covers the outer surface of the elastic balloon, a biological amnion covers the outer surface of the foldable net-pipe-shaped polylactic acid bracket, a plurality of tiny holes are formed in net filaments of the foldable net-pipe-shaped polylactic acid bracket, and biological amnion powder fills the plurality of tiny holes; under the initial state, the elastic balloon, the foldable net-pipe-shaped polylactic acid bracket and the biological amnion are compressed to in the compact state; and under the use state, after the stent is implanted into the body and swelling occurs due to pressurizing, the tube-shaped or waterdrop-similar-shaped state can be formed complying with the lacrimal duct / uterine cavity, or the other spatial states adaptive to body cavity also can be formed.

The invention relates to a polyurethane material subjected to photo-induced graft surface modification by fungi polysaccharide and a preparation method thereof. The polyurethane material subjected to photo-induced graft surface modification by the fungi polysaccharide is characterized by consisting of a base material and a modification layer; the base material consists of a common commercial polyurethane material; and the modification layer is formed by performing photo-induced graft surface modification on the surface of the base material by using fungi polysaccharide, namely lentinan. The surface of the polyurethane material subjected to photo-induced graft surface modification by the fungi polysaccharide has high antibacterial activity, and blood compatibility, so that as the artificial transplant material implanted into a human body, and the biocompatible medical material of an artificial organ and device, the polyurethane material has a wide application range, and the preparationmethod of the material is simple, high in reaction speed, and low in cost and is easy to control.

The invention provides a compound active amnion material, a preparation method and application thereof and a compound active amnion uterine cavity repair stent. The preparation method comprises the following steps: (1) providing a collagen sponge prepare liquid or a compound collagen sponge prepare liquid; (2) providing an amnion with both an epithelial layer and a basement membrane; (3) pouring and coating the prepared prepare liquid on the basement membrane of the amnion to enable the basement membrane and the prepare liquid to be subject to crosslinking, and then preparing the compound active amnion material through freeze drying. The compound active amnion material not only keeps the complete structure and the due biological function of the amnion but also endow the amnion with a certain rigidity, so that the compound active amnion material has stronger maneuverability in application after TCRA (Transcervical Resection Of Adhesions) operation; the compound active amnion uterine cavity repair stent integrates the compound active amnion material and a saccule at the front end of a pipe body, the saccule expands under the action of an external force to push out the compound active amnion material, and then the compound active amnion material is successfully attached to the inner wall of the uterine cavity; the stent is convenient to take out and prevents secondary damage.

The invention discloses a ultra-low temperature conserving solution of animal cell and conserving method thereof for conserving cell especially fresh separated primary animal cell, e.g. liver cell. Culturing fresh separated animal cell at 37 DEG C 0-24 h, changing culture medium as ultra-low temperature conserving solution at 0-4 DEG C then conserving 0-48 months at -80--196 DEG C. After conserving finished, recoverying culturing in a fresh cell culture medium at 37 DEG C, finally culturing in a normal animal cell culture medium. Adding Chinese medicine effective constituent and other chemical protective agent in conserving process and before and after culture medium to improve survival rate of animal cell after conserving at ultra-low temperature and function of cell. The ultra-low temperature conserving technique of separated animal cell is used for conveying and conserving biotype artifical organs in consist of biology artifical liver and other organ cell, also suit for application of animal cell in other medicine field.