Methods and system for amplifying bone marrow mesenchymal stem cells, and computer readable medium

A bone marrow mesenchymal stem cell technology, applied in the field of bone marrow mesenchymal stem cell expansion system and computer readable medium, can solve the problem of decreased proliferation ability, poor biological activity and function, Limitation of BMMSCs research and clinical application and other issues

Pending Publication Date: 2019-09-06
SOUTH CHINA INSTITUDE OF BIOMEDICINE +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the content of BMMSCs in the bone marrow is very small, and as the age of the donor grows, the number of BMMSCs in the body gradually decreases, and the proliferative ability de

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  • Methods and system for amplifying bone marrow mesenchymal stem cells, and computer readable medium
  • Methods and system for amplifying bone marrow mesenchymal stem cells, and computer readable medium
  • Methods and system for amplifying bone marrow mesenchymal stem cells, and computer readable medium

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Embodiment Construction

[0043] Embodiments of the invention are described in detail below, examples of which are illustrated in the accompanying drawings. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0044] The present invention takes the following technical solutions:

[0045] Place the bone marrow mesenchymal stem cells isolated in vitro at 36-38°C, 4.5-5.5% CO 2 , 1.5~2.5% O 2 Cultivate in a low-oxygen incubator for 1-3 days, and then transfer to 36-38°C, 4.5-5.5% CO 2 , O 2 The volume concentration is 15-25% in a normoxic incubator, and the culture medium is α-MEM, 1% glutamine and 5% fetal bovine serum (FBS), and relevant detection is carried out. The specific steps are as follows:

[0046] (1) The bone marrow fluid in rat femur and tibia was separated in vitro, and separated and extracted with rat lymphocyte separation medium with a density of 1.07...

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Abstract

The invention relates to methods and system for amplifying bone marrow mesenchymal stem cells, and a computer readable medium. The method includes performing induction culturing on bone marrow mesenchymal stem cells for 1-3 days under conditions that temperature is at 36-38 DEG C, the volume concentration of N2 is 91-93%, the volume concentration of air is 1%, the volume concentration of CO2 is 4.5-5.5% and the volume concentration of O2 is 1.5-2.5%; and performing continuous culturing on the induction-cultured bone marrow mesenchymal stem cells under the conditions that the temperature is at36-38 DEG C, the volume concentration of CO2 is 4.5-5.5% and the volume concentration of O2 is 15-25% so as to amplify the bone marrow mesenchymal stem cells. The method can rapidly amplify the bone marrow mesenchymal stem cells and obtain the bone marrow mesenchymal stem cells with the best proliferative capacity, biological activity and endothelial differentiation function.

Description

technical field [0001] The present invention relates to the technical field of bioengineering, specifically, the present invention relates to a method, a system and a computer-readable medium for expanding bone marrow mesenchymal stem cells, and more specifically, the present invention relates to a method for expanding bone marrow mesenchymal stem cells, expanding A system and computer readable medium for bone marrow mesenchymal stem cells. Background technique [0002] Bone marrow mesenchymal stem cells (Bone Marrow Mesenchymal Stem Cells, BMMSCs) are a group of heterogeneous cells that exist in the bone marrow. , adipocytes, endothelial cells, etc., and can form a variety of connective tissues, such as skeletal muscle, tendon, ligament, dermis, fat, and bone marrow matrix, etc., which have great potential in tissue regeneration, wound repair, and the treatment of various diseases . However, endothelial cells derived from stem cells have more important and extensive signi...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2500/02
Inventor 周军年裴雪涛黄媛媛岳文周月环
Owner SOUTH CHINA INSTITUDE OF BIOMEDICINE
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