Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods and system for amplifying bone marrow mesenchymal stem cells, and computer readable medium

A bone marrow mesenchymal stem cell technology, applied in the field of bone marrow mesenchymal stem cell expansion system and computer readable medium, can solve the problem of decreased proliferation ability, poor biological activity and function, Limitation of BMMSCs research and clinical application and other issues

Pending Publication Date: 2019-09-06
SOUTH CHINA INSTITUDE OF BIOMEDICINE +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of BMMSCs in the bone marrow is very small, and as the age of the donor grows, the number of BMMSCs in the body gradually decreases, and the proliferative ability decreases, and the biological activity and function deteriorate during the routine culture in vitro. To a certain extent, it limits the further research and clinical application of BMMSCs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods and system for amplifying bone marrow mesenchymal stem cells, and computer readable medium
  • Methods and system for amplifying bone marrow mesenchymal stem cells, and computer readable medium
  • Methods and system for amplifying bone marrow mesenchymal stem cells, and computer readable medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0043] Embodiments of the invention are described in detail below, examples of which are illustrated in the accompanying drawings. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0044] The present invention takes the following technical solutions:

[0045] Place the bone marrow mesenchymal stem cells isolated in vitro at 36-38°C, 4.5-5.5% CO 2 , 1.5~2.5% O 2 Cultivate in a low-oxygen incubator for 1-3 days, and then transfer to 36-38°C, 4.5-5.5% CO 2 , O 2 The volume concentration is 15-25% in a normoxic incubator, and the culture medium is α-MEM, 1% glutamine and 5% fetal bovine serum (FBS), and relevant detection is carried out. The specific steps are as follows:

[0046] (1) The bone marrow fluid in rat femur and tibia was separated in vitro, and separated and extracted with rat lymphocyte separation medium with a density of 1.07...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to methods and system for amplifying bone marrow mesenchymal stem cells, and a computer readable medium. The method includes performing induction culturing on bone marrow mesenchymal stem cells for 1-3 days under conditions that temperature is at 36-38 DEG C, the volume concentration of N2 is 91-93%, the volume concentration of air is 1%, the volume concentration of CO2 is 4.5-5.5% and the volume concentration of O2 is 1.5-2.5%; and performing continuous culturing on the induction-cultured bone marrow mesenchymal stem cells under the conditions that the temperature is at36-38 DEG C, the volume concentration of CO2 is 4.5-5.5% and the volume concentration of O2 is 15-25% so as to amplify the bone marrow mesenchymal stem cells. The method can rapidly amplify the bone marrow mesenchymal stem cells and obtain the bone marrow mesenchymal stem cells with the best proliferative capacity, biological activity and endothelial differentiation function.

Description

technical field [0001] The present invention relates to the technical field of bioengineering, specifically, the present invention relates to a method, a system and a computer-readable medium for expanding bone marrow mesenchymal stem cells, and more specifically, the present invention relates to a method for expanding bone marrow mesenchymal stem cells, expanding A system and computer readable medium for bone marrow mesenchymal stem cells. Background technique [0002] Bone marrow mesenchymal stem cells (Bone Marrow Mesenchymal Stem Cells, BMMSCs) are a group of heterogeneous cells that exist in the bone marrow. , adipocytes, endothelial cells, etc., and can form a variety of connective tissues, such as skeletal muscle, tendon, ligament, dermis, fat, and bone marrow matrix, etc., which have great potential in tissue regeneration, wound repair, and the treatment of various diseases . However, endothelial cells derived from stem cells have more important and extensive signi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2500/02
Inventor 周军年裴雪涛黄媛媛岳文周月环
Owner SOUTH CHINA INSTITUDE OF BIOMEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com