Compound active amnion material, preparation method and application thereof and compound active amnion uterine cavity repair stent

An amniotic membrane and active technology, applied in the field of gynecological intrauterine adhesion treatment, can solve the problems of accelerated postoperative adhesion, secondary injury of metal stents, poor effect, etc., achieving strong operability, good adaptability to the human body, and avoiding secondary damage effect

Active Publication Date: 2013-10-16
JIANGXI RUIJI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a composite active amniotic uterine cavity repair bracket, so as to solve the problem of inflammation caused by the inability to effectively separate the front and rear walls of the uterus in the prior art, and then accelerate postoperative adhesion; the removal of the metal bracket will cause secondary damage to the human body; drug treatment The effect of severe IUA patients is not optimistic, and the postoperative re-adhesion rate is over 50%; it does not have the function of promoting endometrial regeneration and repair; the amniotic membrane cannot be ideally attached to the inner wall of the uterus, resulting in inconvenient surgery and poor results.

Method used

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  • Compound active amnion material, preparation method and application thereof and compound active amnion uterine cavity repair stent
  • Compound active amnion material, preparation method and application thereof and compound active amnion uterine cavity repair stent
  • Compound active amnion material, preparation method and application thereof and compound active amnion uterine cavity repair stent

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preparation example Construction

[0027] 1. Preparation of composite active amnion material

[0028] 1.1 Preparation of amniotic membrane

[0029] The amniotic membrane used in this example is human amniotic membrane, which can be selected from commercialized human amniotic membrane that meets the standards (YZB / National 0593-2005). During the treatment of the amniotic membrane, the amniotic membrane epithelial cells can be retained or the epithelial cells can be removed by enzymatic hydrolysis , no matter whether the epithelial cells are removed or not, the preparation of the composite active amniotic membrane material in the present invention is not affected. Among them, the methods are as follows:

Embodiment 1

[0031] Preparation of amniotic membrane with epithelial cells preserved: In a clean and sterile environment, fresh amniotic membrane is rinsed with sterile saline, with the epithelial layer facing up, spread it on 0.45um microporous nitrocellulose filter paper, and place it in 0.2%~ Soak in 2% glycerin for later use.

Embodiment 2

[0033] Preparation of amniotic membrane without epithelial cells: In a clean and sterile environment, after washing the fresh amniotic membrane with sterile water, use 0.2% to 0.5% trypsin or 0.25% trypsin + 0.02% EDTA (ethylenediaminetetraacetic acid, Ethylene diamine tetraacetic acid), hydrolyzed in a 37°C water bath for 20-120 minutes, removed epithelial cells, fibroblasts, and serous fluid with a cell scraper, and rinsed repeatedly with sterile water. At this time, the decellularized amniotic membrane was a translucent membrane. Stain (hematoxylin-eosin staining method), no epithelial cells remain under optical microscope observation, soak in 0.2%-1% glycerol for later use.

[0034] 1.2 Preparation of collagen sponge or composite collagen sponge preparation solution

[0035] The present invention includes two preparation methods of collagen sponges. The collagen sponges or composite collagen sponges prepared by the following methods are applicable to the present invention....

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Abstract

The invention provides a compound active amnion material, a preparation method and application thereof and a compound active amnion uterine cavity repair stent. The preparation method comprises the following steps: (1) providing a collagen sponge prepare liquid or a compound collagen sponge prepare liquid; (2) providing an amnion with both an epithelial layer and a basement membrane; (3) pouring and coating the prepared prepare liquid on the basement membrane of the amnion to enable the basement membrane and the prepare liquid to be subject to crosslinking, and then preparing the compound active amnion material through freeze drying. The compound active amnion material not only keeps the complete structure and the due biological function of the amnion but also endow the amnion with a certain rigidity, so that the compound active amnion material has stronger maneuverability in application after TCRA (Transcervical Resection Of Adhesions) operation; the compound active amnion uterine cavity repair stent integrates the compound active amnion material and a saccule at the front end of a pipe body, the saccule expands under the action of an external force to push out the compound active amnion material, and then the compound active amnion material is successfully attached to the inner wall of the uterine cavity; the stent is convenient to take out and prevents secondary damage.

Description

technical field [0001] The invention relates to the technical field of gynecological intrauterine adhesion treatment, and more specifically relates to a composite active amniotic membrane material, its preparation method and application, and a composite active amniotic membrane uterine cavity repair bracket. Background technique [0002] Intrauterine adhesions (IUA), also known as Asherman syndrome, refers to the mutual adhesion of the uterine cavity muscle wall and / or cervical canal after the endometrium of the uterine cavity or endocervical canal is damaged due to various factors. Affect menstruation and reproductive function of women of childbearing age. Uterine cavity trauma, infection, uterine deformity, and genetic predisposition during pregnancy and non-pregnancy are the main reasons for the formation of intrauterine adhesions; the main pathological changes of intrauterine adhesions are endometrial fibrosis and scar formation. Clinically, intrauterine adhesions are m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/12A61M31/00
Inventor 傅筱冲郭建军滕志强丁科林苗春云
Owner JIANGXI RUIJI BIOTECH CO LTD
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