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A method of preparing fucoidan having an efficiently customized molecular weight and applications of the fucoidan

A technology for fucoidan and molecular brown algae, which is applied in the directions of pharmaceutical formulations, organic active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of restricting the progress of research and market development of fucoidan, unclear structure, complex ingredients, etc. , to achieve significant blood lipid lowering effect, reduce impurity components, and high price.

Active Publication Date: 2017-06-20
点滴(南京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, due to the limitation of extraction technology, most of the fucoidan researched and produced at home and abroad are macromolecular heteropolysaccharides with complex components and unclear structure, which limits the progress of fucoidan research and market development.

Method used

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  • A method of preparing fucoidan having an efficiently customized molecular weight and applications of the fucoidan
  • A method of preparing fucoidan having an efficiently customized molecular weight and applications of the fucoidan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The specific production process steps of a high-efficiency high-purity uniform low-molecular-weight fucoidan product are as follows:

[0040] (1) Raw material: solid fucoidan product extracted from kelp, the specific method is as follows:

[0041] Take kelp and pulverize it so that the ratio of material to liquid is 1:15-20, bathe in 90°C water for 4 hours, and after centrifugation, take the supernatant and precipitate it with 20% (w / v) calcium chloride solution for 30 minutes, centrifuge, and use the volume of the supernatant The ethanol solution with a fraction of 70-80% was precipitated, then centrifuged and dried to obtain crude fucoidan. It is prepared into a solution according to a mass concentration of 3% (w / v);

[0042] (2) Microwave forced dissolution, microwave frequency at 1500MHz, 4 times of treatment, 5min interval, 8s each time;

[0043] (3) Ultrasonic degradation, the fucoidan solution completely dissolved in step (2) is subjected to ultrasonic treatmen...

Embodiment 2

[0049] The specific production process steps of a high-efficiency high-purity uniform low-molecular-weight fucoidan product are as follows:

[0050] (1) Raw materials: the solid fucoidan product extracted from kelp, which is derived from the crude fucoidan prepared in step (9) of Example 1 in the patent CN106008730A, with a mass concentration of 4% (w / v) prepared as a solution;

[0051] (2) Microwave forced dissolution, microwave frequency at 1000MHz, 5 times of treatment, interval 5min, 10s each time;

[0052] (3) Ultrasonic degradation, the fucoidan solution completely dissolved in step (2) is subjected to ultrasonic treatment to reduce the molecular weight of macromolecular fucoidan, the ultrasonic frequency is 60KHz, and the treatment time is 20min;

[0053] (4) Fractionation of fucoidan, using a high-flow rate agarose gel chromatography (DEAE-Sepharose Fast Flow) classification system, using 0-1.5mol / L NaCl solution as the mobile phase for elution, and the flow rate of t...

Embodiment 3

[0059] The specific production process steps of a high-efficiency high-purity uniform low-molecular-weight fucoidan product are as follows:

[0060] (1) Raw materials: fucoidan solution extracted from kelp, the specific preparation method is as follows:

[0061] Take kelp and crush it, use 5-26g / L calcium chloride solution to make the ratio of solid to liquid 1:8-10, adjust the pH to 2, extract in a water bath at 50-60°C for 1-2 hours, and centrifuge to obtain the supernatant It is the fucoidan solution to be processed;

[0062] (2) Microwave forced dissolution, microwave frequency at 1000MHz, treatment 5 times, interval 5min, 5s each time; (3) Ultrasonic degradation, ultrasonic treatment of fucoidan solution completely dissolved in step (2) to reduce macromolecules For the molecular weight of fucoidan, the ultrasonic frequency is 45KHz, and the processing time is 30min;

[0063] (4) Fractionation of fucoidan, using a high-flow agarose gel chromatography (DEAE-Sepharose Fast...

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Abstract

A method of preparing fucoidan having an efficiently customized molecular weight and applications of the fucoidan are disclosed. The method includes subjecting kelp fucoidan that is adopted as a raw material to microwave dissolution and ultrasonic degradation to obtain a fucoidan degraded solution; grading the fucoidan degraded solution by utilizing high-flow-speed agarose gel chromatography; collecting a solution of low-molecular-weight fucoidan having the target molecular weight; subjecting the solution of fucoidan having other molecular weights to ultrasonic degradation again, performing grading by utilizing the high-flow-speed agarose gel chromatography, and collecting a solution of low-molecular-weight fucoidan having the target molecular weight; combining all target fucoidan solutions; filtering the obtained target fucoidan solution by utilizing a nanofiltration membrane; subjecting a filtration product to desalting and concentration; diluting the fucoidan concentrate again; then subjecting the diluted fucoidan concentrate to nanofiltration, concentration and desalting again; precipitating the fucoidan with an ethanol solution, and washing and drying the fucoidan to obtain a fucoidan product having the low molecular weight. The method keeps active components of the fucoidan to the utmost. The purity of a target product that is a fucoidan product having high purity and a uniform low molecular weight is as high as 95% or above.

Description

technical field [0001] The invention relates to a method for preparing fucoidan with high-efficiency customized molecular weight and its application, belonging to the technical field of polysaccharide production. Background technique [0002] Fucoidan is an anionic water-soluble heteropolysaccharide containing sulfate groups that exists in brown algae or some marine invertebrates. In addition to L-fucose and sulfate groups, its main components also contain galactose, mannose, Xylose, glucose, glucuronic acid and protein etc. Studies have shown that fucoidan has a wide range of biological activities, such as lowering blood lipids, anti-tumor, anti-virus, anti-coagulation, anti-oxidation, immune regulation, etc., and its activity is closely related to its molecular weight and molecular composition. [0003] As a unique bioactive polysaccharide, fucoidan is currently a research hotspot in the field of marine drug development. However, for a long time, due to the limitation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C08B37/04A61K31/737A61P3/06
CPCA61K31/737C08B37/0003C08B37/0063
Inventor 董学前张永刚张军思王伟张艳敏赵林刘建军
Owner 点滴(南京)生物科技有限公司
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