Preparation method of white muscardine silkworm anticancer-activity polysaccharide BBPW-2
A technology of BBPW-2 and anti-cancer activity, which is applied in the field of preparation of BBPW-2 anti-cancer active polysaccharide of Bombyx larvae, can solve the problems of unclear anti-cancer mechanism, hindering the expansion of the medicinal level of Bombyx larvae, etc. Achieve the effect of improving medicinal value, positive social and economic benefits, and uniform purity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Take the silkworm powder passed through an 80-mesh sieve, degrease, remove glycosides and alkaloids according to the above method, add distilled water at a ratio of 1:5 to liquid, treat with ultrasonic waves for 40 min, extract at 100°C for 1 h, and extract Crude polysaccharides; crude polysaccharides were deproteinized by the Sevag method (volume ratio chloroform: n-butanol = 4:1), put on a DEAE-Sepharose Fast Flow ion-exchange chromatography column, eluted with distilled water at a flow rate of 3.0 mL / min, and collected automatically The neutral polysaccharide component BBPW was obtained; the Sephacryl S-300 chromatographic column on BBPW was eluted with distilled water at a flow rate of 1.0 mL / min, collected by an automatic collector, and the eluate of the second elution peak was collected, and reduced Concentrate under reduced pressure; put the concentrated solution on a Sephacryl S-200 chromatography column, elute with distilled water at a flow rate of 1.0 mL / min, a...
Embodiment 2
[0023] Take the silkworm powder passed through a 100-mesh sieve, degrease, remove glycosides and alkaloids according to the above method, add distilled water at a ratio of 1:5 to liquid, treat with ultrasonic waves for 50 min, extract at 100°C for 1.5 h, and extract Crude polysaccharides; crude polysaccharides were deproteinized by the Sevag method (volume ratio chloroform: n-butanol = 4:1), put on a DEAE-Sepharose Fast Flow ion-exchange chromatography column, eluted with distilled water at a flow rate of 3.5 mL / min, and collected automatically The neutral polysaccharide component BBPW was obtained; the Sephacryl S-300 chromatographic column on BBPW was eluted with distilled water at a flow rate of 1.2 mL / min, collected by an automatic collector, and the eluate of the second elution peak was collected, and the eluate was reduced. Concentrate under reduced pressure; put the concentrated solution on a Sephacryl S-200 chromatography column, elute with distilled water at a flow rat...
Embodiment 3
[0025] Take the silkworm powder passed through a 90-mesh sieve, degrease, remove glycosides and alkaloids according to the above method, add distilled water at a ratio of 1:5 to liquid, treat with ultrasonic waves for 40 min, extract at 100°C for 1.2 h, and extract Crude polysaccharides; crude polysaccharides were deproteinized by the Sevag method (volume ratio chloroform: n-butanol = 4:1), put on a DEAE-Sepharose Fast Flow ion-exchange chromatography column, eluted with distilled water at a flow rate of 3.2 mL / min, and collected automatically The neutral polysaccharide component BBPW was obtained; the Sephacryl S-300 chromatographic column on the BBPW was eluted with distilled water at a flow rate of 1.1 mL / min, collected by an automatic collector, and the eluate of the second elution peak was collected, and reduced Concentrate under reduced pressure; put the concentrated solution on a Sephacryl S-200 chromatography column, elute with distilled water at a flow rate of 1.1 ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com