Preparation method of mulberry twig antineoplastic activity polysaccharide RMPW-2
A technology of RMPW-2 and anti-tumor activity, which is applied in the direction of anti-tumor drugs and drug combinations, can solve the problems of unclear medicinal mechanism and hinder the expansion of the medicinal range of Mori twig medicine, so as to improve the medicinal value and positive social Benefits and economic benefits, the effect of uniform purity
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Embodiment 1
[0043] Take the young spring mulberry powder that has passed through a 100-mesh sieve and degrease, remove glycosides and alkaloids according to the above method to obtain the residue of mulberry powder, add double distilled water according to the ratio of 1g: 10mL of solid to liquid, and after ultrasonic treatment for 40min, at 100 ℃ Extraction for 2 hours and then suction filtration, repeat once, combine the filtrate obtained by suction filtration twice, concentrate under reduced pressure at 50°C to 1 / 3 of the original volume, and extract the crude polysaccharide;
[0044]The crude polysaccharide solution was repeatedly deproteinized with Sevage solution until no protein was detected in the iodine color reaction and ninhydrin reaction. The mass ratio of chloroform:n-butanol in the Sevage solution was 4:1, and then the pressure was reduced at 50°C. Concentrate and remove chloroform and n-butanol; add absolute ethanol to the crude polysaccharide solution from which the protein ...
Embodiment 2
[0050] Take the old spring mulberry powder that has been passed through an 80-mesh sieve and degrease, remove glycosides and alkaloids according to the above method to obtain the residue of mulberry powder, add double distilled water according to the ratio of 1g: 10mL of solid to liquid, ultrasonic treatment for 40min, and extract at 100°C Suction filtration for 2 hours, repeat once, combine the filtrates obtained from the two suction filtrations, concentrate under reduced pressure at 50°C to 1 / 3 of the original volume, and extract the crude polysaccharide;
[0051] The crude polysaccharide solution was repeatedly deproteinized with Sevage solution until no protein was detected in the iodine color reaction and ninhydrin reaction. The mass ratio of chloroform:n-butanol in the Sevage solution was 4:1, and then the pressure was reduced at 50°C. Concentrate and remove chloroform and n-butanol; add absolute ethanol to the crude polysaccharide solution from which the protein has been...
Embodiment 3
[0057] Take the old spring mulberry powder that has passed through a 90-mesh sieve and degrease, remove glycosides and alkaloids according to the above method to obtain the residue of mulberry powder, add double distilled water according to the ratio of 1g: 10mL solid-liquid, ultrasonic treatment for 40min, and extract at 100°C Suction filtration for 2 hours, repeat once, combine the filtrates obtained from the two suction filtrations, concentrate under reduced pressure at 50°C to 1 / 3 of the original volume, and extract the crude polysaccharide;
[0058] The crude polysaccharide solution was repeatedly deproteinized with Sevage solution until no protein was detected in the iodine color reaction and ninhydrin reaction. The mass ratio of chloroform:n-butanol in the Sevage solution was 4:1, and then the pressure was reduced at 50°C. Concentrate and remove chloroform and n-butanol; add absolute ethanol to the crude polysaccharide solution from which the protein has been removed, an...
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