Preparation method of mulberry twig antineoplastic activity polysaccharide RMPW-2

A technology of RMPW-2 and anti-tumor activity, which is applied in the direction of anti-tumor drugs and drug combinations, can solve the problems of unclear medicinal mechanism and hinder the expansion of the medicinal range of Mori twig medicine, so as to improve the medicinal value and positive social Benefits and economic benefits, the effect of uniform purity

Inactive Publication Date: 2016-02-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that mulberry polysaccharides have various pharmacological activities such as hypoglycemic, immune regulation, anti-oxidation, anti-tumor, and anti-inflammation, but the medicinal mechanism is still unclear, which hinders the improvement of the medicinal level and the expansion of the medicinal scope of mulberry

Method used

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  • Preparation method of mulberry twig antineoplastic activity polysaccharide RMPW-2
  • Preparation method of mulberry twig antineoplastic activity polysaccharide RMPW-2
  • Preparation method of mulberry twig antineoplastic activity polysaccharide RMPW-2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Take the young spring mulberry powder that has passed through a 100-mesh sieve and degrease, remove glycosides and alkaloids according to the above method to obtain the residue of mulberry powder, add double distilled water according to the ratio of 1g: 10mL of solid to liquid, and after ultrasonic treatment for 40min, at 100 ℃ Extraction for 2 hours and then suction filtration, repeat once, combine the filtrate obtained by suction filtration twice, concentrate under reduced pressure at 50°C to 1 / 3 of the original volume, and extract the crude polysaccharide;

[0044]The crude polysaccharide solution was repeatedly deproteinized with Sevage solution until no protein was detected in the iodine color reaction and ninhydrin reaction. The mass ratio of chloroform:n-butanol in the Sevage solution was 4:1, and then the pressure was reduced at 50°C. Concentrate and remove chloroform and n-butanol; add absolute ethanol to the crude polysaccharide solution from which the protein ...

Embodiment 2

[0050] Take the old spring mulberry powder that has been passed through an 80-mesh sieve and degrease, remove glycosides and alkaloids according to the above method to obtain the residue of mulberry powder, add double distilled water according to the ratio of 1g: 10mL of solid to liquid, ultrasonic treatment for 40min, and extract at 100°C Suction filtration for 2 hours, repeat once, combine the filtrates obtained from the two suction filtrations, concentrate under reduced pressure at 50°C to 1 / 3 of the original volume, and extract the crude polysaccharide;

[0051] The crude polysaccharide solution was repeatedly deproteinized with Sevage solution until no protein was detected in the iodine color reaction and ninhydrin reaction. The mass ratio of chloroform:n-butanol in the Sevage solution was 4:1, and then the pressure was reduced at 50°C. Concentrate and remove chloroform and n-butanol; add absolute ethanol to the crude polysaccharide solution from which the protein has been...

Embodiment 3

[0057] Take the old spring mulberry powder that has passed through a 90-mesh sieve and degrease, remove glycosides and alkaloids according to the above method to obtain the residue of mulberry powder, add double distilled water according to the ratio of 1g: 10mL solid-liquid, ultrasonic treatment for 40min, and extract at 100°C Suction filtration for 2 hours, repeat once, combine the filtrates obtained from the two suction filtrations, concentrate under reduced pressure at 50°C to 1 / 3 of the original volume, and extract the crude polysaccharide;

[0058] The crude polysaccharide solution was repeatedly deproteinized with Sevage solution until no protein was detected in the iodine color reaction and ninhydrin reaction. The mass ratio of chloroform:n-butanol in the Sevage solution was 4:1, and then the pressure was reduced at 50°C. Concentrate and remove chloroform and n-butanol; add absolute ethanol to the crude polysaccharide solution from which the protein has been removed, an...

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Abstract

The invention discloses a preparation method of mulberry twig antineoplastic activity polysaccharide RMPW-2. The method comprises the steps of removing small molecular substances such as glycoside and alkaloid of mulberry twig powder by performing backflow and degreasing on petroleum ether and backflow of ethyl alcohol, then extracting crude polysaccharide by double distilled water, removing protein by the Sevag method, precipitating with ethyl alcohol twice in sequence, separating an acid polysaccharide component by DEAE sepharose gel ion-exchange column chromatography, desalting by propylene sephadex S-100 chromatography, collecting eluant corresponding to the second eluting peak through propylene sephadex S-300 chromatographic purification, concentrating under reduced pressure, and freezing and drying in vacuum to obtain the product. The product prepared in the invention is uniform in purity, has a molecular weight of 5.06*10<3>Da, has an obvious inhibition effect on the growth of human cervical carcinoma cells Hela and human gastric carcinoma cells SGC-7901, has no adverse influence on the growth of human embryo nephrocyte HEK293 and mouse macrophage RAW264.7, and can be used for the development of antitumor products.

Description

technical field [0001] The invention relates to a preparation method of polysaccharide, in particular to a preparation method of mulberry branch polysaccharide RMPW-2 with anti-tumor activity. Background technique [0002] Polysaccharides, also known as polysaccharides, are polar complex macromolecules that are polymerized from monosaccharides and have a degree of polymerization greater than 10. The molecular weight is generally tens of thousands or even millions. Polysaccharides are another biological macromolecule that carries a large amount of biological information after proteins and nucleic acids. They come from fungi, algal lichens, plants, bacteria, animals, etc. A large number of pharmacological and clinical studies have shown that natural polysaccharides have effective anticancer effects, and have less toxic and side effects, showing broad research and application prospects. [0003] Ramulus Mori (Ramulusmori) is the dried branch of the Moraceae plant Morusalba. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P35/00
Inventor 时连根陈亚洁
Owner ZHEJIANG UNIV
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