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Extraction method of hericium erinaceus anti-allergic active polysaccharide

A technology of Hericium erinaceus polysaccharides and active polysaccharides, which can be used in allergic diseases, drug combinations, etc., can solve the problem of low anti-allergic activity of Hericium erinaceus polysaccharides, and achieve the effect of uniform purity

Inactive Publication Date: 2018-02-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the antiallergic activity of Hericium erinaceus polysaccharides

Method used

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  • Extraction method of hericium erinaceus anti-allergic active polysaccharide
  • Extraction method of hericium erinaceus anti-allergic active polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The extraction of Hericium erinaceus anti-allergic activity polysaccharide specifically comprises the following steps:

[0034] (1) After drying Hericium erinaceus, crush and pass through a 80-mesh sieve to obtain Hericium erinaceus powder, take 50g of Hericium erinaceus powder, add 1000mL of distilled water, bathe in 80℃ water for 5h, centrifuge at 3000r / min for 20min, and collect the extract , to obtain the crude extract of Hericium erinaceus polysaccharide;

[0035] (2) The crude extract of Hericium erinaceus polysaccharides was vacuum concentrated to a solid content of 10%, then mixed with Sevage reagent (chloroform:n-butanol=5:1, volume ratio) at a volume ratio of 4:1, and deproteinized 5 times, centrifuge at 3000r / min for 20min each time; after removing the Sevage reagent by vacuum rotary evaporation, mix with D315 macroporous resin at a volume ratio of 10:3, stir for 5h, and filter;

[0036] (3) Transfer the crude extract obtained from the final filtration of st...

Embodiment 2

[0042] The extraction of Hericium erinaceus anti-allergic activity polysaccharide specifically comprises the following steps:

[0043] (1) After drying Hericium erinaceus, crush and pass through an 80-mesh sieve to obtain Hericium erinaceus powder, take 80g of Hericium erinaceus powder, add 2400mL of distilled water, bathe in 85℃ water for 6h, centrifuge at 4000r / min for 30min, and collect the extract , to obtain the crude extract of Hericium erinaceus polysaccharide;

[0044] (2) The crude extract of Hericium erinaceus polysaccharides was vacuum concentrated to a solid content of 14%, then mixed with Sevage reagent (chloroform:n-butanol=5:1, volume ratio) at a volume ratio of 2:1, and deproteinized 6 times, centrifuge at 4000r / min for 10min each time; remove the Sevage reagent by vacuum rotary evaporation, mix with D315 macroporous resin at a volume ratio of 10:4, stir for 6h, and filter;

[0045] (3) Transfer the crude extract obtained from the final filtration of step (2) to...

Embodiment 3

[0051] The extraction of Hericium erinaceus anti-allergic activity polysaccharide specifically comprises the following steps:

[0052] (1) After drying Hericium erinaceus, crush and pass through a 80-mesh sieve to obtain Hericium erinaceus powder, take 60g of Hericium erinaceus powder, add 1500mL of distilled water, bathe in 75℃ water for 5.5h, centrifuge at 3500r / min for 25min, collect and extract solution to obtain the crude extract of Hericium erinaceus polysaccharide;

[0053] (2) The crude extract of Hericium erinaceus polysaccharides was vacuum concentrated to a solid content of 13%, then mixed with Sevage reagent (chloroform: n-butanol = 5:1, volume ratio) at a volume ratio of 3:1, and deproteinized 8 times, centrifuge at 3500r / min for 15min each time; remove the Sevage reagent by vacuum rotary evaporation, mix with D315 macroporous resin at a volume ratio of 5:1, stir for 7h, and filter;

[0054] (3) Transfer the crude extract obtained from the final filtration of ste...

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Abstract

The invention discloses an extraction method of a hericium erinaceus anti-allergic active polysaccharide. According to the extraction method, the hericium erinaceus anti-allergic active polysaccharideis obtained by adopting a technology comprising hot water extraction and DEAE (Diethyl Aminoethanol) Sepharose Fast Flow ion exchange chromatography purification. After the hericium erinaceus anti-allergic active polysaccharide obtained by the extraction method is subjected to high performance gel permeation chromatography detection, the hericium erinaceus anti-allergic active polysaccharide hasa single symmetrical peak and uniform purity; the molecular weight calculated relative to a standard curve is 10kDa; the hericium erinaceus anti-allergic active polysaccharide has an obvious inhibition effect on hyaluronidase and has anti-allergic activity.

Description

technical field [0001] The invention relates to the technical field of preparation of anti-allergic active polysaccharides, in particular to a method for extracting anti-allergic active polysaccharides from Hericium erinaceus. Background technique [0002] Hericium erinaceus (Hericium erinaceus) is a well-known medicinal and edible fungus in China. It has attracted much attention because of its biological activity in the prevention and treatment of gastritis and stomach protection. At present, a variety of active ingredients in Hericium erinaceus have been studied, such as polysaccharides, alkaloids, terpenoids, phenolic acids, etc. Research on the active ingredients of Hericium erinaceus has also expanded from stomach protection, immune regulation, anti-tumor, and anti-oxidation to improving mild cognitive impairment, antibacterial, and anti-inflammatory aspects. However, there are few studies on the antiallergic activity of Hericium erinaceus polysaccharides. The relevan...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/08
CPCC08B37/0003
Inventor 黄惠华易晓敏黄越
Owner SOUTH CHINA UNIV OF TECH
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