Application of sargassum graminifolium polysaccharide extract in improvement of intestinal flora and prevention and treatment of diabetes

A technology for extracting Sargassum grass and polysaccharides, which is applied in the field of metabolic diseases to achieve the effects of improving intestinal flora, improving immunity, and having good biological activity

Active Publication Date: 2015-08-12
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the use of Sargassum polysaccharides to regulate intestinal flora and further prevent and treat metabolic diseases (such as diabetes, obesity, etc.)

Method used

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  • Application of sargassum graminifolium polysaccharide extract in improvement of intestinal flora and prevention and treatment of diabetes
  • Application of sargassum graminifolium polysaccharide extract in improvement of intestinal flora and prevention and treatment of diabetes

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Embodiment 1

[0026] The preparation of embodiment 1 Sargassum grass polysaccharide extract of the present invention (1)

[0027] 1. Method steps

[0028] 1.1 Preparation of Sargassum polysaccharide extract

[0029] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 30°C for 20 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 20-mesh sieve to obtain dry powder of Sargassum grassa, and freeze for future use.

[0030] (2) Add ethanol with a volume fraction of 80% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 3 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder in a drying oven at a drying temperature of 30°C until it reaches a constant wei...

Embodiment 2

[0061] The preparation of embodiment 2 Sargassum grass polysaccharide extract of the present invention (two)

[0062] 1. Method steps

[0063] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 20°C for 24 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 40-mesh sieve to obtain dry powder of Sargassum grassa, and freeze for future use.

[0064] (2) Add ethanol with a volume fraction of 90% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 2 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder in a drying oven at a drying temperature of 20°C until it reaches a constant weight. .

[0065] (3) get dry degreasing Sargassum grassa po...

Embodiment 3

[0077] The preparation of embodiment 3 Sargassum grass polysaccharide extract of the present invention (three)

[0078] 1. Method steps

[0079] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 40°C for 12 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 10-mesh sieve to obtain dry powder of Sargassum pratense, and freeze for future use.

[0080] (2) Add ethanol with a volume fraction of 70% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 4 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder in a drying oven at a drying temperature of 40°C until it reaches a constant weight. .

[0081] (3) get dry degreasing Sargassum grass...

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Abstract

The invention relates to an application of sargassum graminifolium polysaccharide extract in improvement of intestinal flora and prevention and treatment of diabetes. Concretely, the invention provides a sargassum graminifolium polysaccharide extract. A preparation method of the sargassum graminifolium polysaccharide extract comprises the following steps: drying, and crushing, so that sargassum graminifolium dry powder is obtained; carrying out ethanol reflux extraction, so that dry and defatted sargassum graminifolium powder is obtained; extracting the dry and defatted sargassum graminifolium powder by utilizing microwave and taking water as a solvent; separating and purifying extracting solution with a polyethyleneglycol / inorganic salt or ethanol / inorganic salt aqueous two-phase system; and collecting in inorganic salt bottom phase, filtering and concentrating by adopting an ultrafiltration membrane, and drying, so that the sargassum graminifolium polysaccharide extract is obtained. The sargassum graminifolium polysaccharide extract has high purity and good biological activity, and animal experiments verify that the intestinal flora can be obviously improved, and immunity of mouse can be obviously improved, so that the sargassum graminifolium polysaccharide extract can be used for preparing a medicine, health product or food which can be used for improving the intestinal flora and preventing and treating metabolic diseases such as diabetes.

Description

technical field [0001] The invention relates to a polysaccharide extract of Sargassum sargassum, in particular to a new extract of Sargassum sargassum polysaccharide and its application in improving intestinal flora to prevent and treat metabolic diseases such as diabetes. Background technique [0002] Sargassum (Sargassum) is a plant of the Sargassum family of Phaeolophyta Staghornaceae. Sargassum.graminifolium.turn belongs to the genus Sargassum. It grows on rocky reefs in the low tidal zone and subtidal zone. The algal body is green or brown and about 50 cm high. It is distributed in Zhoushan and Xiangshan, Zhejiang in my country. , Fujian, Guangdong and Macao are rich in resources. [0003] Sargassum, like other types of seaweed, is also rich in polysaccharides, brown algae polyphenols, γ-linolenic acid, amino acids and trace elements and other biologically active substances; among them, Sargassum polysaccharide is a kind of easy to extract from Sargassum Water-soluble,...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P3/04A61P3/10A61P37/04A61P1/00A23L1/09A23L1/30A23L2/52A23C9/13
CPCY02A50/30
Inventor 张朝燕缪志刚吴文惠王小雨
Owner SHANGHAI OCEAN UNIV
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