Methods for preparing collagen oleostock and moistening eyedrops

A technology of moisturizing eye solution and collagen, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, peptide/protein components, etc., can solve problems such as dry eye syndrome, drug resistance, irritation, etc., and achieve good curative effect and repair Effect of cornea, good biological activity

Active Publication Date: 2011-09-14
GUANGZHOU TRAUER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, adding preservatives and/or antibiotics can cause side effects such as drug resistance and irritation, and severe cases may cause dry eye syndrome, glaucoma, etc.
[0006] In ophthalmic preparati

Method used

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  • Methods for preparing collagen oleostock and moistening eyedrops
  • Methods for preparing collagen oleostock and moistening eyedrops
  • Methods for preparing collagen oleostock and moistening eyedrops

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] A method for preparing collagen stock solution includes the following steps:

[0031] 1) The raw materials are soaked in a solution of neogermide, and then soaked in a mixed solution of peracetic acid and ethanol for disinfection and harmless treatment;

[0032] 2) Clean the raw materials for disinfection and harmless treatment, and soak in acetic acid solution for 12 to 48 hours to obtain collagen solution.

[0033] Preferably, the concentration of the triglyceride solution is 0.1-0.5%.

[0034] Preferably, the soaking time of the triglyceride solution is 10-25 min.

[0035] Preferably, in the mixed solution of peroxyacetic acid and ethanol, the volume concentration of peroxyacetic acid is 0.1 to 0.3%, and the volume concentration of ethanol is 70 to 75%.

[0036] Preferably, the soaking time of the mixed solution of peroxyacetic acid and ethanol is 10-30 minutes, and more preferably, the soaking time is 10-20 minutes.

[0037] Preferably, the concentration of the acetic acid solu...

Example Embodiment

[0051] Example 1

[0052] 1) Wash the fresh rat tail with water to remove the stains and serum on the serum and fur;

[0053] 2) Soak the cleaned rat tail in 0.5% Tricermide solution for 15 minutes, and after washing, soak in 0.3% peracetic acid combined with 75% ethanol for 10 minutes;

[0054] 3) Peel the rat tail to extract the tendon, wash it, and soak it in 0.05M acetic acid for 48 hours to obtain a collagen solution.

Example Embodiment

[0055] Example 2

[0056] 1) Wash the fresh rat tail with water to remove the stains and serum on the serum and fur;

[0057] 2) Soak the cleaned rat tails in 0.1% neoceramide solution for 25 minutes, and after washing, soak them in 0.1% peracetic acid combined with 70% ethanol for 20 minutes;

[0058] 3) Peel the rat tail to extract the tendon, wash it, soak and extract it in 0.1M acetic acid at room temperature for 36 hours to obtain the collagen solution.

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Abstract

The invention discloses methods for preparing collagen oleostock and moistening eyedrops. The method for preparing collagen oleostok comprises the following steps of: soaking raw materials with bromogeramine; then soaking with the mixed solution of peracetic acid and ethanol for sterilizing and decontaminating; and cleaning the sterilized and decontaminated raw materials and soaking with acetum to obtain the collagen oleostock. The method for preparing moistening eyedrops comprises the following steps of: performing enzymolysis and purification on the collagen oleostock to obtain collagen liquid; adjusting the concentration and osmotic pressure of the collagen liquid, filling under an aseptic condition and finally sealing to obtain disposable moistening collagen eyedrops. By using the method for preparing the collagen oleostock, various microbes and harmful substances in the raw materials can be killed effectively, and collagen cannot be inactivated. The moistening eyedrops prepared by using the method disclosed by the invention has a good treatment effect on dry eye syndrome, can restore cornea, is applicable to eye ball cleaning, lubricating and moistening, routine nursing care on dry eye syndrome and hemostasis and restoration of cornea injury.

Description

technical field [0001] The invention relates to a preparation method of collagen stock solution and eye-moisturizing solution. Background technique [0002] Keratoconjunctival sicca, also known as dry eye, is a type of disease that causes ocular discomfort symptoms due to tear film instability and ocular surface damage caused by abnormal tear quality and quantity or tear fluid dynamics. Dry eye is the cause of many factors, among which, long-term use of eye lotion or care solution containing preservatives is one of the most important causes of eye dry eye syndrome in modern people. [0003] Adding a certain amount of preservatives to the eye lotion is good for the preservation of the preparation, but long-term use can cause superficial irritation or damage to the normal human eye cornea, thereby causing or aggravating dry eye symptoms. As a conventional preservative, brogeramine is widely used in various eye moisturizers, and the general dosage is 0.1g / ml. Some studies have...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61K9/08A61P27/02
Inventor 雷静陈淡嫦林玉泉赵澎黄冬晶佟刚黄胜
Owner GUANGZHOU TRAUER BIOTECH
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