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Method for extracting tremella polysaccharide from tremella fuciformis

A tremella polysaccharide, tremella polysaccharide technology, applied in the field of extraction of natural active product Tremella polysaccharide, can solve the problems of not suitable for large-scale industrial production application, increase extraction equipment investment and energy consumption, etc., achieve safe and efficient extraction method, reduce extraction preparation Cost, good effect of biological activity

Active Publication Date: 2021-06-11
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the emerging new methods mainly include ultrasonic extraction, microwave extraction, and ultra-high pressure extraction. On the one hand, the investment and energy consumption of extraction equipment have been greatly increased. On the other hand, these new laboratory equipment are not Suitable for large-scale industrial production applications

Method used

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  • Method for extracting tremella polysaccharide from tremella fuciformis

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Experimental program
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Effect test

Embodiment 1

[0041] Crush the white fungus and pass through a 120-mesh sieve to get the white fungus powder. Take the tremella powder after sieving and add 0.4% compound enzyme enzymolysis solution (the compound enzyme ratio is cellulase: pectinase: xylanase=1:0.8:1), the pH of compound enzyme enzymolysis solution= 4.5, make the two fully mixed, enzymatic hydrolysis at 55°C for 1 hour, heat up to inactivate the enzyme after enzymatic hydrolysis, heat up to 100°C for 2 hours, after the extraction is completed, the extract is centrifuged at 5000rpm for 10 minutes to remove the residue, and take it up after centrifugation The polysaccharide was determined by the sulfuric acid phenol method in the serum, and the extraction rate was 61.88%.

Embodiment 2

[0043] Crush the white fungus and pass through a 120-mesh sieve to get the white fungus powder. Take the white fungus powder after sieving and add 0.3% compound enzyme enzymolysis solution (the compound enzyme ratio is cellulase: pectinase: xylanase=1:0.8:1.2), the pH of compound enzyme enzymolysis solution= 4.5, make the two fully mixed, enzymatically hydrolyze at 55°C for 1 hour, heat up to inactivate the enzyme after enzymatic hydrolysis, heat up to 100°C for extraction for 2 hours, after the extraction is completed, the extract is centrifuged at 5000rpm for 10min to remove the residue, and take the supernatant after centrifugation The content of tremella polysaccharide was determined by sulfuric acid phenol method, and the extraction rate was 57.62%.

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Abstract

The invention belongs to the field of extraction of a natural active product tremella polysaccharide in tremella fuciformis, and particularly relates to a method for extracting tremella polysaccharide from tremella fuciformis, which comprises the following steps: pretreating the raw material tremella fuciformis, crushing and sieving to obtain tremella fuciformis powder, selecting an efficient and suitable compound enzyme, reacting with the tremella fuciformis powder, and heating for enzyme deactivation treatment after enzymolysis is completed; then extracting at high temperature; performing sufficient stirring in the extraction process to ensure sufficient dissolution of polysaccharide; and after the extraction is completed, centrifugally separating the extracting solution, collecting the supernate, namely the polysaccharide component obtained by extraction, and determining the polysaccharide content by adopting a sulfuric acid-phenol method. The extraction rate of the tremella polysaccharide is greatly improved by combining compound enzyme catalytic conversion extraction with a high-temperature extraction technology; the purity of the extracted tremella polysaccharide is higher than that of tremella polysaccharide obtained through a traditional method, and biological activity is good; and the formula of the obtained specific compound enzyme is optimized, the extraction and preparation cost of the tremella polysaccharide is reduced, the method is suitable for industrial production, and the tremella polysaccharide can be more widely applied to the fields of cosmetics, food, medicines and the like.

Description

technical field [0001] The invention belongs to the field of extracting tremella polysaccharide, a natural active product in tremella, in particular to a method for extracting tremella polysaccharide from tremella. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Tremella fuciformis Berk, also known as white fungus, white fungus, tremella, originated in Tongjiang, Sichuan, and is widely distributed. It has the effects of nourishing yin and lungs, nourishing the stomach and promoting body fluid. Diseases such as cancer and tumor all have effects, and most of the biological activities are related to Tremella polysaccharide, which is a traditional fungal food medicine in my co...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P19/14C08B37/00
CPCC12P19/04C12P19/14C08B37/0003
Inventor 陈伟高磊王毅飞张帆颜聪
Owner QINGDAO AGRI UNIV
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