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Sodium alginate droplet-assisted method for mass spectrometry sample production and ionization of living matter

A technology of sodium alginate and substances, which is applied in the field of mass spectrometry sample preparation and ionization technology of living substances, can solve the problems of application limitations, achieve the effects of enhancing analytical ability, inhibiting sample diffusion, and improving biological activity

Inactive Publication Date: 2018-06-15
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the water-oil two-phase is usually used to form micro-droplets, the oil phase will form the background, so its application is relatively limited.

Method used

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  • Sodium alginate droplet-assisted method for mass spectrometry sample production and ionization of living matter
  • Sodium alginate droplet-assisted method for mass spectrometry sample production and ionization of living matter
  • Sodium alginate droplet-assisted method for mass spectrometry sample production and ionization of living matter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Mass spectrometry sample preparation method based on centrifugal force gel sphere generator

[0024] Use a pipette gun to measure 100ul of the solution to be tested, and add 150ul of PBS solution containing 3% sodium alginate to the solution to be tested. After mixing the two solutions, take a small amount of the mixed solution and add figure 1 In the sample tube 3 with a needle, add 200 μL of 1% CaCl to the centrifuge tube 4 2 Then start the motor 1, adjust the rotation speed to 4000 rpm, and centrifuge for 1 minute to obtain the required gel ball containing the sample in the centrifuge tube 4.

Embodiment 2

[0025] Example 2: Gel Spheroid Mass Spectrometry Analysis of Hemoglobin

[0026] Weigh 0.002g of hemoglobin (taken from pig blood), dissolve it in 1mL of PBS for later use; use PBS to prepare a sodium alginate solution with a mass fraction of 3%; prepare a mass fraction of NaCl 0.9%, CaCl 2A 1% aqueous solution (curing solution 5) was used for later use. Weigh 0.01g of hemoglobin (taken from pig blood), dissolve it in 1mL of PBS, and use it as a comparative experiment sample.

[0027] In the experiment, 100 μL of hemoglobin solution was added to 200 μL of sodium alginate solution, and mixed. Add 200 µL of solidification solution 5 to centrifuge tube 4 in Figure 1, and put away sample tube 3 with a needle. Take an appropriate amount of sodium alginate solution of hemoglobin and add it into the sample tube 3 with a needle, select a rotating speed of 4000 rpm, and carry out centrifugation for sample preparation to make sodium alginate sample balls with a diameter of about 150 μ...

Embodiment 3

[0029] Embodiment 3: DNA gel sphere mass spectrometry analysis

[0030] Take a 10OD DNA sample and dissolve it in 1mL of PBS for later use; use PBS to prepare a sodium alginate solution with a mass fraction of 2% for later use; prepare an aqueous solution (solidification solution 5) with a mass fraction of 0.9% NaCl and 1% CaCl2 for later use.

[0031] In the experiment, 100 μL of DNA solution was added to 200 μL of sodium alginate solution, and mixed. Towards figure 1 Add 200 μL of solidification solution to the centrifuge tube, and put away the sample tube 3 with a needle. Take an appropriate amount of sodium alginate solution of DNA and add it to the sample tube 3 with a needle, select a rotating speed of 4000 rpm, and carry out centrifugation for sample preparation to make a sodium alginate sample ball with a diameter of about 150 μm for future use.

[0032] Take a gel ball containing DNA and place it at the tip of the spray capillary, then inject methanol water (V:V=1:1...

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Abstract

The invention discloses a method for preparing a mass spectrometry organism complex sample. An organism sample is mixed with sodium alginate, and the obtained mixture is centrifuged and injected to obtain gel beads containing the sample to be detected, used as a mass spectrometry object, so the mass spectrometry sample processing process of the organism sample is simplified, and the use of chemical solvents is reduced, thereby the biological properties of the organism sample during analysis are guaranteed. Analysis of proteins, DNA and cells by using the method proves that the method can be used to perform simple processing in order to obtain effective organism matter information through the mass spectrometry.

Description

technical field [0001] The invention relates to biological sample mass spectrometry technology, in particular to life substance mass spectrometry sample preparation and ionization technology assisted by gel microfluidic droplet technology. Background technique [0002] Since its birth in 1912, mass spectrometry has become an indispensable key method for analytical science with its ultra-high resolution capability of charge-to-mass ratio. Its analysis objects gradually develop from small molecules to macromolecules, from simple systems to complex systems. In particular, the invention of various biological soft ionization techniques including MALDI and ESI in the 1990s accelerated the application of mass spectrometry in the field of life science research. Due to the special analysis principle of mass spectrometry, compared with other analytical methods, mass spectrometry has unique advantages in high-content analysis of complex systems, especially in the face of life systems ...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 徐静娟王姜徐碧漪赵微陈洪渊
Owner NANJING UNIV
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