Sodium alginate droplet-assisted method for mass spectrometry sample production and ionization of living matter

A technology of sodium alginate and substances, which is applied in the field of mass spectrometry sample preparation and ionization technology of living substances, can solve the problems of application limitations, achieve the effects of enhancing analytical ability, inhibiting sample diffusion, and improving biological activity

Inactive Publication Date: 2018-06-15
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the water-oil two-phase is usually used to form micro-droplets, t

Method used

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  • Sodium alginate droplet-assisted method for mass spectrometry sample production and ionization of living matter
  • Sodium alginate droplet-assisted method for mass spectrometry sample production and ionization of living matter
  • Sodium alginate droplet-assisted method for mass spectrometry sample production and ionization of living matter

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1: Mass spectrometry sample preparation method based on centrifugal force gel ball generator

[0024] Use a pipette to measure 100ul of the test solution, and add 150ul of PBS solution containing 3% sodium alginate to the test solution. After mixing the two solutions, add a small amount of the mixed solution figure 1 In the sample tube 3 with the needle in the middle, add 200μL containing 1% CaCl to the centrifuge tube 4 2 Physiological saline, and then start the motor 1, adjust the speed to 4000 rpm, and centrifuge for 1 minute to obtain the desired gel ball containing the sample in the centrifuge tube 4.

Example Embodiment

[0025] Example 2: Gel Ball Mass Spectrometry Analysis of Hemoglobin

[0026] Weigh out 0.002g hemoglobin (taken from pig blood) and dissolve it in 1 mL of PBS for use; use PBS to configure a 3% sodium alginate solution for use; configure a mass fraction of NaCl 0.9%, CaCl 2 1% aqueous solution (solidified solution 5) for use. Weigh out 0.01 g hemoglobin (taken from pig blood) and dissolve it in 1 mL of PBS as a comparative test sample.

[0027] In the experiment, 100 μL of hemoglobin solution was added to 200 μL of sodium alginate solution and mixed. Add 200 μL of solidification solution 5 to the centrifuge tube 4 in Figure 1, and place the sample tube 3 with a needle. Take an appropriate amount of sodium alginate solution of hemoglobin and add it to the sample tube 3 with a needle, select a rotation speed of 4000 rpm, and perform centrifugal sample preparation to prepare a sodium alginate sample ball with a diameter of about 150 μm for use.

[0028] Take a gel ball containing hem...

Example Embodiment

[0029] Example 3: DNA gel ball mass spectrometry analysis

[0030] Take a DNA sample of 10 OD and dissolve it in 1 mL of PBS for use; use PBS to prepare a 2% sodium alginate solution for use; prepare an aqueous solution of NaCl 0.9% and CaCl2 1% (solidification solution 5) for use.

[0031] In the experiment, 100 μL of DNA solution was added to 200 μL of sodium alginate solution and mixed. to figure 1 Add 200μL of solidification solution to the middle centrifuge tube, and place the sample tube 3 with a needle. Take an appropriate amount of DNA sodium alginate solution and add it to the sample tube 3 with a needle, select a rotation speed of 4000 rpm, and perform sample preparation by centrifugation to prepare a sodium alginate sample ball with a diameter of about 150 μm for use.

[0032] Take a gel ball containing DNA and place it on the tip of the spray capillary, then inject methanol-water (V:V=1:1) solution into the capillary, and connect the ionization source electrode, and the ...

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Abstract

The invention discloses a method for preparing a mass spectrometry organism complex sample. An organism sample is mixed with sodium alginate, and the obtained mixture is centrifuged and injected to obtain gel beads containing the sample to be detected, used as a mass spectrometry object, so the mass spectrometry sample processing process of the organism sample is simplified, and the use of chemical solvents is reduced, thereby the biological properties of the organism sample during analysis are guaranteed. Analysis of proteins, DNA and cells by using the method proves that the method can be used to perform simple processing in order to obtain effective organism matter information through the mass spectrometry.

Description

technical field [0001] The invention relates to biological sample mass spectrometry technology, in particular to life substance mass spectrometry sample preparation and ionization technology assisted by gel microfluidic droplet technology. Background technique [0002] Since its birth in 1912, mass spectrometry has become an indispensable key method for analytical science with its ultra-high resolution capability of charge-to-mass ratio. Its analysis objects gradually develop from small molecules to macromolecules, from simple systems to complex systems. In particular, the invention of various biological soft ionization techniques including MALDI and ESI in the 1990s accelerated the application of mass spectrometry in the field of life science research. Due to the special analysis principle of mass spectrometry, compared with other analytical methods, mass spectrometry has unique advantages in high-content analysis of complex systems, especially in the face of life systems ...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 徐静娟王姜徐碧漪赵微陈洪渊
Owner NANJING UNIV
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