Method for preparing hemostatic sponge from oxidized bletilla striata modified collagen fibers

A technology of collagen fibers and hemostatic sponges, which is applied in the field of biomedical materials, can solve problems such as poor bonding, poor mutual bonding ability, and poor degradation resistance, and achieve the effects of improving collagen stability, thermal stability, and hemostatic performance

Pending Publication Date: 2022-03-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, directly mixing Bletilla striata polysaccharides with collagen has the disadvantages of poor combination and easy loss
[0006] In order to overcome the shortcomings of poor collagen degradation resistance and long hemostasis time, etc., and at the same time break through the defects of poor mutual binding ability between Bletilla striata and collagen, the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Purification of Bletilla striata polysaccharide: Weigh 20 parts by weight of Bletilla striata polysaccharide, dissolve it in 1000 parts by weight of deionized water, and stir it magnetically for 1 hour to completely dissolve it; accurately weigh 1 part by weight of papain (enzyme activity: 800,000 u / g), added to the crude polysaccharide solution of Bletilla striata, stirred by magnetic force for 1h, after it was completely dissolved, 1mol / L NaOH was added to it to make the pH to 7; under the condition of 48℃ water bath, enzymatic hydrolysis for 2h; after the reaction, add 1000 parts by weight of absolute ethanol, allowing Bletilla striata polysaccharide to precipitate out in flocculent form; centrifuge at 8000r / min for 5min, collect the precipitate, redissolve in water, remove insolubles by suction filtration and collect the filtrate; centrifuge at 8000r / min again, Repeat 4-5 times, place the final product in a constant temperature drying oven and dry to constant we...

Embodiment 2

[0040] (1) Purification of Bletilla striata polysaccharide: Weigh 20 parts by weight of Bletilla striata polysaccharide, dissolve it in 400 parts by weight of deionized water, and stir it magnetically for 0.5 h to dissolve it completely; weigh 0.5 part by weight of papain (enzyme activity 100,000 u / g), added to the crude polysaccharide solution of Bletilla striata, stirred by magnetic force for 0.5h, after it was completely dissolved, 1mol / L NaOH was added to it to make the pH to 6; Add 1000 parts by weight of absolute ethanol to allow Bletilla striata polysaccharide to precipitate in flocculent form; centrifuge at 6000r / min for 5min, collect the precipitate, redissolve it in water, remove insoluble matter by suction filtration and collect the filtrate; centrifuge again at 6000r / min , repeated 4-5 times, the final product was placed in a constant temperature drying oven and dried to a constant weight to obtain purified Bletilla striata polysaccharide;

[0041] (2) Preparation...

Embodiment 3

[0044] (1) Purification of Bletilla striata polysaccharide: Weigh 20 parts by weight of Bletilla striata polysaccharide, dissolve it in 1200 parts by weight of deionized water, and stir it magnetically for 2 hours to completely dissolve it; accurately weigh 1.5 parts by weight of papain (enzyme activity: 800,000 u / g), added to the crude polysaccharide solution of Bletilla striata, stirred magnetically for 2 hours, after it was completely dissolved, 1mol / L NaOH was added to it to make the pH 8; under the condition of 65℃ water bath, enzymatic hydrolysis for 3 hours, after the reaction, add 1000 parts by weight of absolute ethanol, allowing Bletilla striata polysaccharide to precipitate out in flocculent form; centrifuge at 10000r / min for 5min, collect the precipitate, redissolve in water, remove insolubles by suction filtration and collect the filtrate; centrifuge again at 10000r / min, Repeat 4-5 times, place the final product in a constant temperature drying oven and dry to con...

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PUM

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Abstract

The invention discloses a method for preparing hemostatic sponge from oxidized bletilla striata modified collagen fibers. The preparation method comprises the following steps: oxidizing bletilla striata polysaccharide by adopting sodium periodate to prepare dialdehyde bletilla striata polysaccharide containing cross-linked active aldehyde groups, and chemically modifying collagenous fibers by taking the dialdehyde bletilla striata polysaccharide as a functional cross-linking agent. In the cross-linking process, the three-strand spiral structure of collagen is not damaged, and the porous three-dimensional network structure brings excellent filling power and water absorption performance. The blood coagulation activity of bletilla striata polysaccharide in the hemostatic sponge is well maintained, the platelet adhesiveness is remarkably improved, various blood coagulation indexes are superior to those of most of similar products, and the hemostatic performance is excellent. Meanwhile, the hemostatic sponge has excellent stability, the hemolysis rate meets the national safety requirement, the cytocompatibility is good, and the hemostatic sponge has the effects of promoting tissue growth, promoting wound healing and the like, and is an excellent novel hemostatic material.

Description

technical field [0001] The invention relates to a method for preparing a hemostatic sponge by oxidizing bletilla striata modified collagen fibers, which belongs to the field of biomedical materials. Background technique [0002] Wound bleeding caused by emergency accidents such as car accidents and wars in various occasions is often difficult to control. Bleeding from wounds is also considered the leading cause of death at the scene. According to multidisciplinary statistics, about 39% of deaths in accidents are caused by bleeding, most of which are due to hemorrhagic shock. Therefore, rapid and effective hemostasis of the wounded is extremely important, and shortening the hemostasis time of materials and improving the quality of hemostasis have become the best strategies to reduce the mortality of patients. In-depth research on various hemostatic materials has been widely carried out, among which collagen stands out due to its superior performance, and has gradually attra...

Claims

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Application Information

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IPC IPC(8): C08J9/28C08L89/00C08L5/00A61L24/00A61L24/08A61L24/10
CPCC08J9/28A61L24/08A61L24/102A61L24/0036A61L24/0042C08J2389/00C08J2405/00C08J2305/00A61L2400/04C08L5/00C08L89/00
Inventor 但年华闫星雨陈一宁
Owner SICHUAN UNIV
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