Preparation of lotus seed protein polypeptide

A technology of protein polypeptide and lotus seed, which is applied in the field of preparation of lotus seed protein polypeptide, can solve the problems of difficult industrial production and large investment in equipment, and achieve the effects of cost saving, good taste and avoiding bitterness

Inactive Publication Date: 2009-08-05
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microwave and ultrasonic extraction methods are currently difficult to industrialize, and CO 2 Acid precipitation requires high-pressure equipment, and the equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1

[0017] The coreless white lotus seeds are soaked in water at 30℃ for 2.5 hours. The lotus seeds and water are evenly added to the refiner at a mass ratio of 1:2.5 for wet grinding. The fineness of the refining is 50 mesh; 0.10% Ca(OH) 2 The lotus seed powder was extracted from the solution for 2.0 hours, the mass ratio of material to liquid was 1:8.0, and the filtrate was filtered. The filtrate was centrifuged at a centrifugal speed of 2000 rpm and centrifuged for 20 minutes to obtain the supernatant; the supernatant was adjusted to pH 4.5 with 0.10N HCl. Let stand for 1.0h and centrifuge for 12min to obtain lotus seed protein precipitate; add water to the lotus seed protein precipitate at 40℃ to adjust the concentration of the protein solution to 15%, pH 5.5, the weight of papain is 4.5% of the protein weight, and the enzyme After decomposing for 100 minutes, adjust the supernatant with 0.05N HCl to pH 4.3, let stand for 0.8 h, and centrifuge for 30 min to obta...

Example Embodiment

[0018] Example 2

[0019] The coreless white lotus seeds are soaked in water at 35°C for 3 hours. The lotus seeds and water are evenly added to the refiner at a mass ratio of 1:2.5 for wet grinding. The fineness of the refining is 60 mesh; 0.15% Ca(OH) is used 2 The lotus seed powder was extracted from the solution for 3.0h, the mass ratio of material to liquid was 1:10.0, and the filtrate was filtered. The filtrate was centrifuged at a centrifugal speed of 4000rpm and centrifuged for 30min to obtain the supernatant; the supernatant was adjusted to pH 4.2 with 0.15N HCl. Let it stand for 1.0 h and centrifuge for 10 min to obtain lotus seed protein precipitate. At 40 ℃, add water to the lotus seed protein precipitate to adjust the concentration of the protein solution to 20%, pH 5.5, the weight of the acid protease is 3.5% of the protein weight, after 60 minutes of enzymolysis, adjust the supernatant to pH 4 with 0.05N HCl. 2. Let stand for 1.0 h and centrifuge for 20 min to obtain...

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PUM

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Abstract

The invention discloses a preparation method of lotus seed protein polypeptide. Lotus seed protein is extracted by using a low-concentration weak base Ca (OH)2 to replace a strong base NaOH, so as to greatly reduce the influence of the strong base to raw materials and reduce the denaturalization of protein glomeration and residual lotus seed starch during the extracting process; the extracted protein has good color and taste; meanwhile, lotus nut amylum can be taken as the raw materials of other lotus seed foods additionally. In addition, low-concentration of Ca ion is more advantageous to human beings to absorb the protein, can also make up calcium deficiency and is not required to be removed; and desalting step is not needed during the extracting process of protein. In addition, the pH is not required to be adjusted during the hydrolytic process of acid protease; the hydrolysis process is simple, and the callback is not required simultaneously, the desalting process is eliminated, so that the cost is saved greatly. The hydrolysis of the acid protease is proper, so that the bitterness of products caused by the generation of a large amount of small peptide after excessive hydrolysis is avoided. The lotus seed protein polypeptide has the yield of 71.4 percent and good color and taste.

Description

technical field [0001] The invention relates to a preparation method of lotus seed protein polypeptide. Background technique [0002] Both lotus seed protein and soybean protein are high-quality vegetable proteins with high nutritional value and rich in lysine, and lysine is the first limit in common food or diet (especially in fruits, vegetables and grains with high starch content). Insufficient lysine intake can be supplemented in the diet of some specific groups of people; at the same time, it contains bean seed protein, which generally lacks methionine. The content and proportion of essential amino acids in lotus seed protein are close to the FAO / WHO recommended model, and the proportion of amino acids is relatively balanced, which can reduce the content of cholesterol and saturated fatty acids in the diet, maintain a balanced nutrition, and prevent sub-health and non-communicable diseases. In particular, the protein polypeptide not only absorbs quickly, but also has a ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 曾虹燕蔡联辉王亚举夏葵贺永凌刘翔
Owner XIANGTAN UNIV
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