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Preparation of lotus seed protein polypeptide

A technology of protein polypeptide and lotus seed, which is applied in the field of preparation of lotus seed protein polypeptide, can solve the problems of difficult industrial production and large investment in equipment, and achieve the effects of cost saving, good taste and avoiding bitterness

Inactive Publication Date: 2009-08-05
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microwave and ultrasonic extraction methods are currently difficult to industrialize, and CO 2 Acid precipitation requires high-pressure equipment, and the equipment investment is relatively large
It is worth mentioning that there are no reports or patent disclosures about the preparation process of lotus seed protein polypeptide in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Soak the heart-removed white lotus seeds in water at a temperature of 30°C for 2.5 hours, add the lotus seeds and water to the refiner evenly at a mass ratio of 1:2.5 for wet grinding, and the refining fineness is 50 mesh; use 0.10% Ca(OH) 2 Extract the lotus seed powder with the solution for 2.0 hours, the mass ratio of solid to liquid is 1:8.0, filter to obtain the filtrate, centrifuge the filtrate at a centrifugal rate of 2000 rpm, and centrifuge for 20 minutes to obtain the supernatant; use 0.10N HCl to adjust the supernatant to pH 4.5, Set aside to settle for 1.0 h, centrifuge for 12 min to obtain the lotus seed protein precipitate; at 40°C, add water to the lotus seed protein precipitate to adjust the concentration of the protein solution to 15%, pH 5.5, the weight of papain is 4.5% of the protein weight, the enzyme After solution for 100min, adjust the supernatant to pH 4.3 with 0.05N HCl, let it settle for 0.8h, and centrifuge for 30min to obtain the supernatant ...

Embodiment 2

[0019] Soak the heart-removed white lotus seeds in water at a temperature of 35°C for 3 hours, add the lotus seeds and water into the refiner evenly at a mass ratio of 1:2.5 for wet grinding, and the fineness of the pulp is 60 mesh; use 0.15% Ca(OH) 2 Extract the lotus seed powder with the solution for 3.0 hours, the mass ratio of solid to liquid is 1:10.0, filter to obtain the filtrate, centrifuge the filtrate, centrifuge at a speed of 4000 rpm, and centrifuge for 30 minutes to obtain the supernatant; use 0.15N HCl to adjust the supernatant to pH 4.2, Set aside to settle for 1.0h, and centrifuge for 10min to obtain the lotus seed protein precipitate. At 40°C, add water to the lotus seed protein precipitate to adjust the concentration of the protein solution to 20%, pH5.5, the weight of acidic protease is 3.5% of the protein weight, after 60 minutes of enzymolysis, adjust the supernatant to pH4. 2. Set aside to settle for 1.0 h, centrifuge for 20 min to obtain the supernatant ...

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Abstract

The invention discloses a preparation method of lotus seed protein polypeptide. Lotus seed protein is extracted by using a low-concentration weak base Ca (OH)2 to replace a strong base NaOH, so as to greatly reduce the influence of the strong base to raw materials and reduce the denaturalization of protein glomeration and residual lotus seed starch during the extracting process; the extracted protein has good color and taste; meanwhile, lotus nut amylum can be taken as the raw materials of other lotus seed foods additionally. In addition, low-concentration of Ca ion is more advantageous to human beings to absorb the protein, can also make up calcium deficiency and is not required to be removed; and desalting step is not needed during the extracting process of protein. In addition, the pH is not required to be adjusted during the hydrolytic process of acid protease; the hydrolysis process is simple, and the callback is not required simultaneously, the desalting process is eliminated, so that the cost is saved greatly. The hydrolysis of the acid protease is proper, so that the bitterness of products caused by the generation of a large amount of small peptide after excessive hydrolysis is avoided. The lotus seed protein polypeptide has the yield of 71.4 percent and good color and taste.

Description

technical field [0001] The invention relates to a preparation method of lotus seed protein polypeptide. Background technique [0002] Both lotus seed protein and soybean protein are high-quality vegetable proteins with high nutritional value and rich in lysine, and lysine is the first limit in common food or diet (especially in fruits, vegetables and grains with high starch content). Insufficient lysine intake can be supplemented in the diet of some specific groups of people; at the same time, it contains bean seed protein, which generally lacks methionine. The content and proportion of essential amino acids in lotus seed protein are close to the FAO / WHO recommended model, and the proportion of amino acids is relatively balanced, which can reduce the content of cholesterol and saturated fatty acids in the diet, maintain a balanced nutrition, and prevent sub-health and non-communicable diseases. In particular, the protein polypeptide not only absorbs quickly, but also has a ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 曾虹燕蔡联辉王亚举夏葵贺永凌刘翔
Owner XIANGTAN UNIV
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