Preparation of lotus seed protein polypeptide

[0016] Example 1

[0017] The coreless white lotus seeds are soaked in water at 30℃ for 2.5 hours. The lotus seeds and water are evenly added to the refiner at a mass ratio of 1:2.5 for wet grinding. The fineness of the refining is 50 mesh; 0.10% Ca(OH) 2 The lotus seed powder was extracted from the solution for 2.0 hours, the mass ratio of material to liquid was 1:8.0, and the filtrate was filtered. The filtrate was centrifuged at a centrifugal speed of 2000 rpm and centrifuged for 20 minutes to obtain the supernatant; the supernatant was adjusted to pH 4.5 with 0.10N HCl. Let stand for 1.0h and centrifuge for 12min to obtain lotus seed protein precipitate; add water to the lotus seed protein precipitate at 40℃ to adjust the concentration of the protein solution to 15%, pH 5.5, the weight of papain is 4.5% of the protein weight, and the enzyme After decomposing for 100 minutes, adjust the supernatant with 0.05N HCl to pH 4.3, let stand for 0.8 h, and centrifuge for 30 min to obtain the lotus seed protein polypeptide supernatant; quickly heat the protein polypeptide supernatant to 88°C and keep it for 10 minutes. The protein peptide solution is concentrated to a concentration of 25%; the material solution temperature is 60°C, the spray inlet temperature is 150°C, and the spray outlet temperature is 68°C. The lotus seed protein polypeptide powder is obtained by spray drying.

[0018] Example 2

[0019] The coreless white lotus seeds are soaked in water at 35°C for 3 hours. The lotus seeds and water are evenly added to the refiner at a mass ratio of 1:2.5 for wet grinding. The fineness of the refining is 60 mesh; 0.15% Ca(OH) is used 2 The lotus seed powder was extracted from the solution for 3.0h, the mass ratio of material to liquid was 1:10.0, and the filtrate was filtered. The filtrate was centrifuged at a centrifugal speed of 4000rpm and centrifuged for 30min to obtain the supernatant; the supernatant was adjusted to pH 4.2 with 0.15N HCl. Let it stand for 1.0 h and centrifuge for 10 min to obtain lotus seed protein precipitate. At 40 ℃, add water to the lotus seed protein precipitate to adjust the concentration of the protein solution to 20%, pH 5.5, the weight of the acid protease is 3.5% of the protein weight, after 60 minutes of enzymolysis, adjust the supernatant to pH 4 with 0.05N HCl. 2. Let stand for 1.0 h and centrifuge for 20 min to obtain the lotus seed protein polypeptide supernatant; quickly heat the protein polypeptide supernatant to 80°C, keep it for 15 minutes, inactivate the protease, and concentrate the protein polypeptide solution to a concentration of 20%; The liquid temperature is 65°C, the spray inlet temperature is 180°C, and the spray outlet temperature is 75°C. The lotus seed protein polypeptide powder is obtained by spray drying.