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Chemiluminescence detection kit of magnetic particles of placental growth factors and preparation method thereof

A placental growth factor, chemiluminescence detection technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, measurement devices, etc., can solve the problems of electrode replacement price, high background signal, insufficient sensitivity, etc. Achieve the effect of low cost, high signal-to-noise ratio and low background luminescence

Inactive Publication Date: 2018-01-05
太原瑞盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the enzyme-linked immunoassay is cheap and fast, it is not sensitive enough and is only suitable for the detection and identification of trace substances; electrochemiluminescence requires the use of three electrodes to carry out an unknown complex reaction between multiple electroactive samples. The electrode needs to be replaced and is expensive, the electrochemiluminescence method has a high background signal due to the electrochemiluminescence caused by the high concentration of TPA, etc.

Method used

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preparation example Construction

[0022] The present invention provides a magnetic particle chemiluminescence detection kit for placental growth factor and a preparation method. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below.

[0023] The present invention provides a magnetic particle chemiluminescence detection kit for placental growth factor and a preparation method, wherein, the magnetic particle chemiluminescence method detection kit for placental growth factor provided by the present invention adopts magnetic particle coupling placental growth factor capture antibody , Acridinium ester-labeled placental growth factor detection antibody. The kit also includes placental growth factor calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution for the above-mentioned acridinium ester action.

[0024] Specifically, the magnetic part...

Embodiment 1

[0034] Embodiment 1: The set-up and preparation method of a magnetic particle chemiluminescence kit for detecting placental growth factor comprises the following steps:

[0035] 1. The composition of the kit:

[0036] A magnetic particle chemiluminescent kit for detecting placental growth factor was constructed so that it contained the following components:

[0037] Magnetic particle-coupled placental growth factor monoclonal antibody;

[0038] Another placental growth factor monoclonal antibody labeled with acridinium ester;

[0039] Placenta growth factor series standard solution;

[0040] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0041] Cleaning fluid.

[0042] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Placental Growth Factor Monoclonal Antibody

[0043] (1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifuge tube, add 200 μL of MES buffer solution with a concentration of 0.1 mol / L, vo...

Embodiment 2

[0063] Embodiment 2: The steps for detecting using the magnetic particle chemiluminescent detection kit of placental growth factor are:

[0064] (1) Add 50 μL of the sample to be tested into the cuvette, then add 150 μL of magnetic particle coupling suspension, shake and mix, and incubate at 37°C for 8 min.

[0065] (2) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0066] (3) Add 150 μL of acridinium ester marker into the cuvette, shake to mix, and incubate at 37°C for 7 min.

[0067] (4) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0068] (5) Add 100 μL of chemiluminescence pre-excitation solution A, add 100 μL of chemiluminescence excitation solution B after 1 s, and measure its relative luminous intensity. The content of placental growth factor in the sample is proportional to its relative luminous intensity.

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Abstract

The invention discloses a magnetic particle chemiluminescence detection kit for placental growth factor and a preparation method. The kit includes: magnetic particles coupled with placental growth factor capture antibody, acridinium ester-labeled placental growth factor detection antibody, placental growth factor calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses magnetic separation chemiluminescence technology as a detection method and combines it with acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time, and higher accuracy and repeatability of detection results. The kit can be applied to various luminescence detection instruments.

Description

Technical field [0001] The invention belongs to the technical field of immune detection and analysis, and is specifically an immunomagnetic particle chemiluminescence detection kit for placental growth factor and a preparation method. Background technique [0002] The molecular structure of placental growth factor (PLGF) is a glycoprotein homodimer molecule. Its base sequence is highly homologous to that of VEGF. 53% of its protein amino acid composition is the same as that of VEGF. It is a new member of the VEGF family. member. PLGF currently has four different protein isoforms: PLGF-1, PLGF-2, PLGF-3 and PLGF-4. All four isoforms are produced by human trophoblasts and umbilical vein endothelial cells. PLGF regulates the functions of trophoblasts and endothelial cells in the placenta through autocrine and paracrine forms. During the remodeling process of uterine spiral arteries, syncytiotrophoblast cells secrete placental growth factor (PlGF) to promote angiogenesis and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N21/76
Inventor 许殊荣程月萍李瑶杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
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