Screening kit for seventh chromosome abnormality diseases of fetus

A chromosomal abnormality and kit technology, applied in the field of kits, can solve problems such as unbalanced gametes, and achieve the effects of simple operation, high patient compliance, and good application prospects.

Active Publication Date: 2013-07-17
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] An abnormality of chromosome 7, such as an inversion between the arms, can produce unbalanced gametes, which can lead to Turner syndrome

Method used

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  • Screening kit for seventh chromosome abnormality diseases of fetus
  • Screening kit for seventh chromosome abnormality diseases of fetus
  • Screening kit for seventh chromosome abnormality diseases of fetus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 sample collection

[0019] 1. Collection object: A total of 35 pregnant women in the first trimester visited the outpatient clinic of West China Second Hospital of Sichuan University and the Second Affiliated Hospital of Chengdu University of Traditional Chinese Medicine from October 2011 to April 2012.

[0020] 2. Diagnostic criteria for early pregnancy: refer to the method recorded in the 7th edition of the textbook "Obstetrics and Gynecology" of national higher medical colleges (including ① history of menopause; ② HCG positive; gestational sac).

[0021] 3. Inclusion criteria: ① Those who meet the above diagnostic criteria; ② Gestational age: 5 weeks to 13+6 weeks; ③ Informed consent, voluntary subjects.

[0022] 4. Exclusion criteria: patients with cardiovascular, liver, kidney and hematopoietic system complications, or other primary diseases and mental illness.

[0023] 5. Drop-out criteria: ① Cases that do not meet the inclusion criteria or are accompa...

Embodiment 2

[0029] The detection method of embodiment 2PLGF level

[0030] 1. Main instruments

[0031] (1) Microplate reader: Molecular Devices, Spectra Max5

[0032] (2) Refrigerated centrifuge: BECKMAN company, AllegraTM64R

[0033] (3) -20°C Refrigerator: Electrolux Company

[0034] (4) Ultra-low temperature refrigerator: Thermo company, SK501

[0035] (5) Electric constant temperature incubator: Shanghai Senxin Experimental Instrument Co., Ltd., DRP-9162

[0036] 2. Experimental reagents and materials

[0037] (1) Capture Antibody: mouse anti-human PLGF monoclonal antibody Human PLGF Antibody (Monoclonal Mouse IgG1), purchased from R&D Company, MAB264;

[0038] (2) Detection Antibody: Biotin-labeled goat anti-human PLGF polyclonal antibody HumanPLGF Biotinylated Antibody (Ployclonal Goat IgG), purchased from R&D Company, BAF264;

[0039] (3) PLGF standard product: recombinant human PLGF Recombinant Human PLGF, purchased from R&D Company, 264-PG-10;

[0040] (4) Streptavidin-ho...

Embodiment 3

[0060] Example 3 Correlation between PLGF level and fetal chromosome 7 abnormality

[0061] The blood and urine samples collected in Example 1 were detected respectively according to the method of Example 2, and the statistical results are shown in Tables 1-2:

[0062] Table 1 Correlation between fetal chromosome 7 abnormality and serum PLGF level

[0063]

[0064] As shown in Table 1, when the fetus is normal, the serum PLGF of the pregnant woman is 228.07pg / ml, and when the fetal chromosome 7 is abnormal, the serum PLGF of the pregnant woman is as low as 15.6pg / ml, the former is 212.47pg / ml higher than the latter, which is the 14.62 times.

[0065] Table 2 Correlation between fetal chromosome 7 abnormality and urinary PLGF level

[0066]

[0067] As shown in Table 2, when the fetus is normal, the urine PLGF of the pregnant woman is 164.66pg / ml, and when the fetus has abnormal chromosome 7, the urine PLGF of the pregnant woman is 105.23pg / ml, the former is 59.43pg / ml ...

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Abstract

The invention discloses a screening kit for the seventh chromosome abnormality diseases of a fetus. The screening kit comprises a reagent for detecting the PLGF (placental growth factor) level of the serum or urine of a pregnant woman. The invention further discloses an application for the screening kit for the seventh chromosome abnormality diseases of a fetus in detection for the PLGF level of the serum or urine of a pregnant woman. The kit disclosed by the invention can be used for evaluating whether the seventh chromosome of the fetus is normal or not, and is good in clinical application prospect.

Description

technical field [0001] The invention relates to a reagent kit, in particular to a screening reagent kit for fetal chromosome 7 abnormal disease. Background technique [0002] Human chromosome 7 is one of 23 pairs of chromosomes. Under normal conditions, each cell has two. This chromosome contains about 1000 to 1400 genes, accounting for 5% to 5.5% of all DNA in the cell. [0003] Abnormalities of chromosome 7, such as interarm inversion, can produce unbalanced gametes, which in turn cause Turner syndrome. The growth rate of patients with Turner syndrome is normal in the first 2 to 3 years of life, and then it is lower than that of normal women. During puberty, normal women have a growth acceleration, while patients with Turner syndrome are still growing slowly, and normal women are about 15 to 16 years old. The height basically stops growing at the age of 18, while the Turner syndrome grows slowly until about 18 years old. The average height of adults with Turner syndrome ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 陆华高小平刘小章
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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