Rabbit spinal tuberculosis animal model building method based on twelfth rib positioning

Abstract

The invention discloses a rabbit spinal tuberculosis animal model building method based on twelfth rib positioning. The method comprises the following steps of randomly selecting a plurality of healthy white rabbits with unlimited male and female and weight of 2.8-3.0kg, randomly selecting one white rabbit, weighing, and performing ear marginal vein anesthesia by using pentobarbital; and fixing the white rabbit anesthetized in the step 1 on an animal operation plate, and then performing a series of operations to complete modeling. According to the rabbit spinal tuberculosis animal model building method based on the twelfth rib positioning, the multiple white rabbits are used for conducting anesthesia in sequence and then conducting an operation, bone parts are punched, tubercle bacillus samples are implanted, then wounds are sewn, cage-separated feeding is conducted, a contrast test is conducted, the states of the white rabbits after the test are observed after the white rabbits are bred for a period of time, and vertebral bodies of rabbits were examined by X-ray, MRI imaging, specimen pathology, bacteriology and the like. The method has the advantages of being easy to locate, lowin cost, practical in technology, short in period, convenient to model, high in success rate and the like, and the basic performance after tubercle bacillus infection can be clearly determined by combining the general situation of animals, the typical imaging performance and the combined bacterium culture situation.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a method for building a rabbit spinal tuberculosis animal model based on the positioning of the 12th rib. Background technique [0002] Bone and joint (tuberculosis of) Mycobacterium tuberculosis invades the human body through the respiratory tract or digestive tract, forming a primary focus, where Mycobacterium tuberculosis enters the lymphatic blood line and spreads to various organs throughout the body, especially the reticuloendothelial system Including bones and joints, most disseminated foci are eliminated by phagocytes, while very few disseminated and latent. Once the body's resistance is reduced, the tuberculosis bacteria in the latent infection foci multiply and break through the surrounding tissues to cause disease. [0003] Adolescents are the most common cases, usually solitary, often occurring in the spine, followed by knees, hips, and elbows. The onset is slo...

AI Technical Summary

Problems solved by technology

[0004] In clinical medicine, due to the particularity of the pathological structure of bone and joint tuberculosis, it is difficult for anti-tuberculosis drugs to exert a strong bactericidal effect on bone tissue