The invention relates to a preservation method for fresh lycium barbarum. The preservation method for the fresh lycium barbarum includes the following steps of (1) taking the fresh lycium barbarum, washing the fresh lycium barbarum, removing impurities on the surface of the fresh lycium barbarum, soaking the fresh lycium barbarum in ozone solution with concentration of 0.5-1.0 mg / L for 30-40s, draining the fresh lycium barbarum, washing the fresh lycium barbarum with purified water, draining the fresh lycium barbarum, blow-drying the surface of the fresh lycium barbarum, putting the fresh lycium barbarum in a preservation plate for later use, (2) putting every 5-8kg of the fresh lycium barbarum processed through the step (1) into a freshness protecting package, putting the packaged fresh lycium barbarum with the opening of the package opened into a refrigeration house, precooling the fresh lycium barbarum, putting a pack of first preservatives and a pack of second preservatives into the freshness protecting package after the temperature of the core of the fresh lycium barbarum reaches 0-1 DEG C, tightening the opening of the package, putting the packaged fresh lycium barbarum into the refrigeration house with the temperature ranged from minus 2 DEG C to minus 1 DEG C, and refrigerating fresh lycium barbarum. The preservation method for the fresh lycium barbarum is low in process cost, simple and convenient to operate and practical in technology, and prolongs the preservation period of the fresh lycium barbarum.