Natural wood fiber carrier for microbial immobilization and preparing method of natural wood fiber carrier

A technology of microorganism immobilization and wood fiber, applied in the direction of microorganism-based methods, biochemical equipment and methods, immobilized on/in organic carriers, etc., can solve the problems of application limitations, unstable gel carriers, etc., and achieve Ease of application, avoidance of toxic effects or adverse effects, technically practical effects

Inactive Publication Date: 2017-07-07
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most commonly used immobilization method is gel fixation such as calcium alginate, and the gel carrier is in CO 2 It is unstable when it exists, so its application in related processes such as microbial ethanol fermentation is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for preparing a natural lignocellulosic carrier for immobilizing brewer's yeast, which uses corn stalks as a substrate and is prepared by a method comprising the following steps:

[0039] 1) Select relatively uniform corn stalks, cut them into cubes with a side length of 1 cm or cylinders with a diameter of 1 cm and a height of 1 cm, wash them with deionized water, and dry them at 75°C for more than 24 hours to constant weight.

[0040] 2) Add deionized water into the custom-made 600mL high-temperature and high-pressure reactor, and add 20 to 30 groups of corn stalks pretreated in step 1), the total volume of the added corn stalks is 1 / 3 of the effective volume of the high-temperature and high-pressure reactor, The total volume of the added corn stalks and water is 1 / 2 of the effective volume of the high temperature and high pressure reactor.

[0041] 3) Provide hydrothermal reaction conditions for the above-mentioned high-temperature and high-pressure reactor....

Embodiment 2

[0045] A method for preparing a natural lignocellulosic carrier for immobilizing brewer's yeast, which uses corn stalks as a substrate and is prepared by a method comprising the following steps:

[0046] 1) Select corn stalks with a relatively uniform material, cut them into cubes with a side length of 2 cm or cylinders with a diameter of 2 cm and a height of 2 cm, wash with deionized water and dry at 75°C for more than 24 hours to constant weight.

[0047] 2) Add deionized water into the custom-made 600mL high-temperature and high-pressure reactor, and add 20 to 30 groups of corn stalks pretreated in step 1), the total volume of the added corn stalks is 1 / 3 of the effective volume of the high-temperature and high-pressure reactor, The total volume of the added corn stalks and water is 1 / 2 of the effective volume of the high temperature and high pressure reactor.

[0048] 3) Provide hydrothermal reaction conditions for the above-mentioned high-temperature and high-pressure rea...

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Abstract

The invention relates to the field of microbial fermentation, in particular to a natural wood fiber carrier for microbial immobilization and a preparing method of the natural wood fiber carrier. The method includes the following steps that (1) natural wood fiber materials relatively even in texture are selected, and mechanical pretreatment is carried out on the natural wood fiber materials; (2) water is added into a high temperature and high pressure reaction still, and then the natural wood fiber materials treated in the step (1) are added; (3) the natural wood fiber materials obtained in the step (2) and water are jointly heated and pressurized to the appointed temperature and appointed pressure so as to make a hydrothermal reaction, and the hydrothermal reaction is continuously made for appointed time; (4) after the hydrothermal reaction in the step (3) is finished, the temperature and pressure in the high temperature and high pressure reaction still are decreased, and the natural wood fiber materials obtained after hydrothermal treatment are taken out; and (5) the natural wood fiber materials obtained in the step (4) are washed and dried to obtain the natural wood fiber carrier. By means of the natural wood fiber carrier and the preparing method, the microorganism fixing effect is improved, significance in microbial immobilization is achieved, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a natural wood fiber carrier for immobilizing microorganisms and a preparation method thereof. Background technique [0002] In various processes based on microbial fermentation, the reaction efficiency of free microorganisms is relatively low, and it is easily inhibited by harmful substances in the matrix, which often becomes one of the key factors restricting the promotion of the process. Microbial immobilization is a common method to increase the biomass of the reaction system and protect the microorganisms from harmful components, and it is easier for product separation and process control, and has been widely used in various microbial fermentation processes. The most commonly used immobilization method is gel fixation such as calcium alginate, and the gel carrier is in CO 2 It is unstable when it exists, so its application in related processes such as microbi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B27K5/00C12N11/02C12R1/865
CPCB27K5/0085C12N11/02
Inventor 赵岩徐颖杰刘珊谭豪波
Owner BEIJING NORMAL UNIVERSITY
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