46 results about "Beta-hemolytic streptococcus" patented technology

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

The invention belongs to the medical technical field and discloses the application of high-purity in the preparation of antibacterial medicine, antiviral medicine and other medicines. The invention proves the antipyretic, anti-inflammation, immunity enhancement, antibacterial and anti-virus effects of high-purity forsythiaside through the following experiments: (1) the experiment that high-purity forsythiaside has antipyretic effect on fever rats; (2) the experiment that high-purity forsythiaside inhibits xylene-induced mice ear edema, xylene-reduced capillary permeability of mice; (3) the experiment that high-purity forsythiaside increases carbon clearance ability of mice and increases the lymphocyte transformation rate; (4) the experiment that high-purity forsythiaside has vitro anti-influenza A type virus effect and in-vivo anti-respiratory syncytial virus effect; (5) the experiment that high-purity forsythiaside has vitro inhibition effects on beta-hemolytic streptococcus, staphylococcus aureus, streptococcus viridans and staphylococcus epidermidis.

The invention discloses an essential oil composition prepared by performing rectification and compounding processing on the raw materials comprising 4-terpilenol type tea-tree-oil and eucalyptus grandis x e.urophylla essential oil, and provides the formula, a preparation method and the composition content of the essential oil composition; and the composition has antibacterial insecticidal functions. A gas chromatography method is employed to determine the main content. The method is accurate, stable and easy to operate, is adaptable for industrial production demands, and can help to effectively control the quality of the essential oil composition. The essential oil composition has small toxicity, has different levels of inhibition effects on six pathogens comprising staphylococcus aureus, staphylococcus epidermidis, beta hemolytic streptococcus, escherichia coli, pseudomonas aeruginosa and candida albicans which can cause skin infection, and has antibacterial and insecticidal activities. The preparation method is reasonable and stable, short in production period, suitable for industrial production, and good in application prospect.

The invention relates to sterilizing and antiviral plant essential oil and a preparation method thereof. The method is characterized in that effective components are respectively extracted from plantssuch as eaglewood, ravensara, eucalyptus, rosemary, tea tree, bergamot and lavender; main components are linalool, cineol, pinene, bronyl acetate, linalyl alkene, fenchene, pomelo terpene, eucalyptol, terpineol, limonene and dipentene. The essential oil is selected from a variety of components extracted from various plants and does not contain phenolic substances, so that toxicity, harms and sideeffects on a human body are avoided; moreover, a variety of active ingredients are synergized with one another according to certain concentration and proportion to realize an obvious inhibitory effect on various bacteria and viruses such as influenza virus (H3N2, H7N9 and hepatitis B), pneumobacillus, beta hemolytic streptococcus, pseudomonas aeruginosa and mold.

A reagent is provided for the detection of an exotoxin protein produced by a beta-hemolytic streptococcus bacteria suspected of being present in a host biological fluid collected from a subject. An enzyme inhibitor is present to inhibit rogue protein modification of the substrate to prevent a false positive result of the color change. A kit is provided that is readily usable by an untrained user and merely requires that an element of the kit be contacted with a biological sample and thereafter no further actions are required by the user before a discernable color change is observed with visible or UV light and a positive/negative results reference card.

The invention relates to a lignin compound that could prevent yellow staphylococcal bacteria and B type hemolytic streptococcus, and the medicine combination of the compound. The invention of lignin Morgan dandehon herb acid A and Rufescidride has obviously effect restraining yellow staphylococcal and B type hemolytic streptococcus. It is also respected to prevent the diseases caused by them, like boil, blain, toxicity epidermis necrolysis, pneumonia, etc.

This invention relates to Ligulatrovine A separated from Ligularia actroviolacea that can prevent and treat diseases related to Staphylococcus aureus and beta hemolytic streptococcus, its pharmaceutical salts and drug composition. Ligulatrovine A has significant inhibitive effect on Gram-positive bacteria, and can prevent and treat diseases such as boil, pustule, pneumonia, osteomyelitis, acute myocarditis, endocarditis, meningitis, mastitis, cystitis, pelvic inflammation, urinary inflammation, prostatitis, bacteremia, or abscess in muscle, skin, urogential region or central nervous system caused by Gram-positive bacteria infection.

The invention discloses a method for quickly detecting beta-hemolytic streptococcus in a textile product. The method is characterized in that two technologies of IMS (immunomagnetic separation) and RPA (recombinase ploymerase amplification) are combined, and the beta-hemolytic streptococcus in a sample is enriched by an immunomagnetic bead capturing technology; one set of specific primer and probeusing the RPA technology as the core technology is designed for the target gene with the specificity HtrA of the beta-hemolytic streptococcus, and the enriched beta-hemolytic streptococcus is detected by the RPA technology. The method has the advantages that the culture is not needed in the whole process; the time is shortened, the operation is quick, the efficiency is high, the specificity is good, the sensitivity is high, the anti-interference property is strong, the application range is broad, and the detection cost is low; the method is not only suitable for sanitation health and epidemicprevention departments and health and supervision departments, and but also suitable for the sanitation detection in enterprises and families.

The invention discloses a flavonoid glycoside and medical salt and application to prevent Micrococcus pyogenes and beta hemolytic streptococcus, which is characterized by the following: preventing pustule, toxic epidermal decay, acute myocarditis, cystitis, cervicitis and tumour of neutral nerve system; fitting for treating prostatitis, pneumococcus, medullitis and knuckle disease.

The present invention is directed to a method for the ultrasensitive detection of beta hemolytic Streptococcus, a bacterium implicated in strep throat, using a specific protease marker. Also disclosed is a device as well as a biosensor, both of which are useful for the detection of beta hemolytic Streptococcus. The biosensor and the device can be used in conjunction with other reagents as part of a kit for detecting strep throat.

The invention discloses applications of Phyllanthus emblica or Phyllanthus emblica and a Phyllanthus emblica composition in preparation of products for preventing and/or treating at least one of mastitis, diarrhea and porcine respiratory diseases, and further provides antibacterial effects of Phyllanthus emblica or Phyllanthus emblica extract and the Phyllanthus emblica composition, wherein the Phyllanthus emblica or Phyllanthus emblica extract and the Phyllanthus emblica composition can effectively inhibit the growth of one or a variety of bacteria selected from Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas, Salmonella choleraesuis, Escherichia coli, Group A beta-hemolytic streptococcus, Streptococcus suis, Bordetella, Pasteurella multocida, Salmonella enterica, Staphylococcus haemolyticus, Enterococcus faecalis and Erysipelothrix rhusiopathiae, and the effectiveness of the uses are proved through experiments so as to expanded the applications of Phyllanthus emblica andthe Phyllanthus emblica composition.

The invention belongs to the field of traditional Chinese medicines, in particular relates to a traditional Chinese medicine for treating acne and a preparation method thereof. The traditional Chinese medicine is refined by thirteen traditional Chinese medicines of flos farfarae, coptis chinensis, honeysuckle, scutellaria baicalensis, prepared rehmannia root, moutan bark, salvia miltiorrhiza, radix rehmanniae recen, jasmine, radix scrophulariae, thunberg fritillary bulb, cortex mori and selfheal. All the medicines are compatible with each other perfectly to achieve the effect of clearing away heat, relieving poison, cooling blood and activating blood; the traditional Chinese medicine has obvious counteraction effect on the acute and chronic inflammation, has obviously antibacterial effect on the common skin pathogenic bacterium of staphylococcus aureus, staphylococcus epidermidis, beta hemolytic streptococcus, escherichia coli, pseudomonas aeruginosa and propionibacterium, can obviously reduce the swelling rate of the propionibacterium caused mouse auricle acne model, obviously improves the activity of catalase to achieve the anti-oxidation effect, and has a certain pain relieving effect; the traditional Chinese medicine has the advantages of short treatment course, quick effect and no toxic or side effects, and is a good medicine for treating the acne.

The invention provides a test strip for detecting group A B hemolytic streptococcus with high sensitivity. The detection sensitivity is high, the color development speed is high, a tracer conjugate pad and a sample pad are unified into an antibody-red latex microsphere marker integrated pad by processing glass fibers, forming of a water storage area is avoided, the tracer release speed is increased, the interpretation time is shortened, and one production process is reduced, so that the labor cost is saved; the invention also provides a kit for high-sensitivity detection of group A B hemolyticstreptococcus, which is convenient to use and simple to operate, and can prevent error results caused by improper mixing sequence or mixing proportion. The invention further provides a detection method for detecting the group A B hemolytic streptococcus with high sensitivity, and the method is simple, high in operability and short in consumed time.

The invention discloses a method for rapidly extracting an egg yolk antibody, an oral ulcer-resistant product prepared thereby and application thereof. The method comprises the following steps: immunizing laying hens by beta hemolytic streptococcus, escherichia coli and candida albicans all-bacteria vaccine; mechanically automatically separating eggs to obtain yolk; diluting a citric acid-disodiumhydrogen phosphate buffer solution and stabilizing the pH value of yolk liquid; adding dry ice, rapidly reducing the system temperature, performing continuous high-speed low-temperature centrifugation and removing fat particles to obtain yolk antibody supernatant; enabling the yolk antibody supernatant to pass through double-layer filter membranes of 2 to 6 um and 0.3 to 0.8 um and a hollow fiberultrafiltration membrane with molecular weight cut-off of 300 kD to remove impure protein as well as pass through a hollow fiber ultrafiltration membrane with molecular weight cut-off of 100 kD to obtain concentrated yolk antibody liquid; and adding auxiliary materials to prepare oral mucosal spray. The method has the advantages of high automation degree, low energy consumption, high yield, environmental friendliness, capability of realizing large-scale industrialized production and the like.

The invention is suitable for the field of microbial technology and provides a staphylococcus aureus and hemolytic streptococcus detection kit and a detection method. The kit comprises a DNA extract and a PCR reaction solution. The staphylococcus aureus and hemolytic streptococcus detection kit and the detection method provided by the invention can be used to simultaneously detect staphylococcus aureus and hemolytic streptococcus. In comparison with a traditional culture method, the detection method is rapid, sensitive and safe, and can be applied for detection of staphylococcus aureus and hemolytic streptococcus.

The invention provides a water-extraction preparation method and an application of an antibacterial active component in bamboo willow. Bamboo willow bark powder is subjected to ultrasonic extraction 1-5 times with distilled water diluted by 5-20 times and for 30-120 min each time, and an extraction solution is obtained; the extraction solution is subjected to alcohol precipitation to remove water-soluble impurities and then separated with macroporous resin, 95% ethanol elution sections are collected, concentrated under reduced pressure and purified with Sephadex LH-20 to remove pigments, and the antibacterial active component in the bamboo willow is obtained. The bamboo willow water extract has better inhibition activity for bacteria such as beta hemolytic streptococcus, staphylococcus aureus, bacilli, escherichia coli, pseudomonas aeruginosa and the like and can serve as a prescribed preparation to be directly used for treating diseases caused by bacterial infection, such as acute tonsillitis, acute pharyngitis, acute laryngitis and acute otitis media. With the adoption of the method, the antibacterial active component is successfully extracted from the bamboo willow and a foundation is laid for application of the bamboo willow in the field of medicine products.

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses an antibacterial, anti-inflammatory and antiviral traditional Chinese medicine composition and a preparation method and application thereof, and the traditional Chinese medicine composition is prepared from the following components in parts by weight: 10-20 parts of honeysuckle, 2-8 parts of mint, 3-9 parts of safflower, 6-12 parts of hawthorn, 15-25 parts of bunge corydalis herb, 6-12 parts of moutan bark, 3-9 parts of monkshood and 6-10 parts of eucommia ulmoides. The traditional Chinese medicine composition prepared by the invention has relatively strong inhibition on staphylococcus aureus, escherichia coli and beta hemolytic streptococcus, and has the best inhibition effect on the staphylococcus aureus.