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Staphylococcus aureus and hemolytic streptococcus detection kit and detection method

A technology of hemolytic streptococcus and detection kit, which is applied in the field of microorganisms and can solve problems such as long cycle

Active Publication Date: 2012-11-07
深圳生科原生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0025] In view of this, the present invention provides a detection kit for Staphylococcus aureus and hemolytic streptococcus, which solves the technical problems of low sensitivity and long cycle for simultaneous detection of Staphylococcus aureus and hemolytic streptococcus in the prior art, and gold Detection method of staphylococcus aureus and hemolytic streptococcus

Method used

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  • Staphylococcus aureus and hemolytic streptococcus detection kit and detection method
  • Staphylococcus aureus and hemolytic streptococcus detection kit and detection method

Examples

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Embodiment 1

[0081] The embodiment of the present invention Staphylococcus aureus, hemolytic streptococcus detection method comprises the following steps:

[0082] 1. Collect samples of Staphylococcus aureus and hemolytic streptococcus. The number of samples is 20, some of which contain Staphylococcus aureus and hemolytic streptococcus, and some samples do not contain Staphylococcus aureus and hemolytic streptococcus. Place the collected specimens in a constant temperature room at 37°C for 3 hours of enrichment culture; then perform the following DNA lysis treatment:

[0083] Take 1ml of the enrichment solution and add 50μL of the DNA extraction solution, and repeatedly aspirate 3 times to fully mix the specimen to obtain the DNA samples of Staphylococcus aureus and hemolytic streptococcus;

[0084] 2. Take the above PCR reaction solution, each component in the PCR reaction solution is:

[0085] first reaction solution

[0086]

[0087]

[0088] Second reaction solution

[0089] T...

Embodiment 2

[0097] The embodiment of the present invention Staphylococcus aureus, hemolytic streptococcus detection method comprises the following steps:

[0098] 1. Collect samples of Staphylococcus aureus and hemolytic streptococcus. The number of samples is 20, some of which contain Staphylococcus aureus and hemolytic streptococcus, and some samples do not contain Staphylococcus aureus and hemolytic streptococcus. Place the collected specimens in a constant temperature room at 37°C for 3 hours of enrichment culture; then perform the following DNA lysis treatment:

[0099] Take 1ml of the enrichment solution and add 50μL of the DNA extraction solution, and repeatedly aspirate 3 times to fully mix the specimen to obtain the DNA samples of Staphylococcus aureus and hemolytic streptococcus;

[0100] 2. Take the above PCR reaction solution, each component in the PCR reaction solution is:

[0101] first reaction solution

[0102]

[0103] Second reaction solution

[0104] Taq enzyme 0.2 ...

Embodiment 3

[0112] The embodiment of the present invention Staphylococcus aureus, hemolytic streptococcus detection method comprises the following steps:

[0113] 1. Collect samples of Staphylococcus aureus and hemolytic streptococcus. The number of samples is 20, some of which contain Staphylococcus aureus and hemolytic streptococcus, and some samples do not contain Staphylococcus aureus and hemolytic streptococcus. Place the collected specimens in a constant temperature room at 37°C for 3 hours of enrichment culture; then perform the following DNA lysis treatment:

[0114] Take 1ml of the enrichment solution and add 50μL of the DNA extraction solution, and repeatedly aspirate 3 times to fully mix the specimen to obtain the DNA samples of Staphylococcus aureus and hemolytic streptococcus;

[0115] 2. Take the above PCR reaction solution, each component in the PCR reaction solution is:

[0116] first reaction solution

[0117]

[0118] Second reaction solution

[0119] Taq enzyme 0....

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Abstract

The invention is suitable for the field of microbial technology and provides a staphylococcus aureus and hemolytic streptococcus detection kit and a detection method. The kit comprises a DNA extract and a PCR reaction solution. The staphylococcus aureus and hemolytic streptococcus detection kit and the detection method provided by the invention can be used to simultaneously detect staphylococcus aureus and hemolytic streptococcus. In comparison with a traditional culture method, the detection method is rapid, sensitive and safe, and can be applied for detection of staphylococcus aureus and hemolytic streptococcus.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a detection kit and a detection method for staphylococcus aureus and hemolytic streptococcus. Background technique [0002] Staphylococcus aureus is an important pathogenic bacterium of humans, which belongs to the genus Staphylococcus, has another name of "fleshophilus", and is a representative of Gram-positive bacteria. The pathogenicity of Staphylococcus aureus mainly depends on the invasive enzymes and toxins it produces, which can cause local suppurative infection, pneumonia, pseudomembranous enteritis, pericarditis, etc., and even systemic infection such as sepsis and sepsis , The symptoms of poisoning are severe, mainly manifested as vomiting, fever, and diarrhea. [0003] Hemolytic streptococci are widely distributed in nature and exist in water, air, dust, feces, and the mouth, nose, and throat of healthy humans and animals. They can be infected throu...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/14C12R1/445C12R1/46
Inventor 卢柳燕龚剑田仁鹏吴成贡汪再兴黄娟
Owner 深圳生科原生物有限公司
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