Water-extraction preparation method and application of antibacterial active component in bamboo willow

An antibacterial activity and water extraction technology is applied in the field of water extraction and preparation of antibacterial active components of bamboo and willow. , the effect of increasing the added value

Active Publication Date: 2017-05-31
BIOLOGY INST OF SHANDONG ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this invention, only salicin in bamboo willow is extracted, but other active ingredients in bamboo willow are not extracted.
And in the prior art, there is no relevant report...

Method used

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  • Water-extraction preparation method and application of antibacterial active component in bamboo willow
  • Water-extraction preparation method and application of antibacterial active component in bamboo willow
  • Water-extraction preparation method and application of antibacterial active component in bamboo willow

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of bamboo willow antibacterial active component comprises the following steps:

[0031] A) Extraction: Precisely weigh 100g of bamboo willow bark powder that has been crushed and passed through a 24-mesh sieve, add ten times the amount of distilled water, and perform ultrasonic extraction for 3 times, each time for 90 minutes, with an ultrasonic frequency of 18KHz and an ultrasonic temperature of 50°C, suction filtration, and extraction Liquid merge;

[0032] B) Alcohol precipitation: the extract is concentrated under reduced pressure to 50 mL, slowly add 140 mL of 95% ethanol to make the alcohol content of the extract reach 70%, store at 4°C for 18 hours, centrifuge, take the supernatant, and concentrate under reduced pressure to no alcohol taste;

[0033] C) Separation of macroporous resin: AB-8 type macroporous resin wet-packed column, diameter-to-height ratio 1:12, step B) obtained concentrated solution wet loading, with distilled water, volume frac...

Embodiment 2

[0037] The preparation of bamboo willow antibacterial active component comprises the following steps:

[0038] A) Extraction: Accurately weigh 100g of bamboo willow skin powder that has been crushed and passed through a 40-mesh sieve, add 5 times the amount of distilled water, and perform ultrasonic extraction for 3 times, 60 minutes each time, with an ultrasonic frequency of 12KHz and an ultrasonic temperature of 55°C, suction filtration, and extraction Liquid merge;

[0039] B) Alcohol precipitation: the extract is concentrated under reduced pressure to 50 mL, slowly add 120 mL of 95% ethanol to make the alcohol content of the extract reach 60%, store at 4°C for 24 hours, centrifuge, take the supernatant, and concentrate under reduced pressure to no alcohol taste;

[0040] C) Separation of macroporous resin: AB-8 type macroporous resin wet-packed column, diameter-to-height ratio 1:12, step B) obtained concentrated solution wet loading, with distilled water, volume fraction ...

Embodiment 3

[0044] The preparation of bamboo willow antibacterial active component comprises the following steps:

[0045] A) Extraction: Accurately weigh 100g of bamboo willow bark powder that has been crushed and passed through a 10-mesh sieve, add 20 times the amount of distilled water, perform ultrasonic extraction 4 times, each time 90min, ultrasonic frequency 9KHz, ultrasonic temperature 70°C, suction filtration, extraction Liquid merge;

[0046] B) Alcohol precipitation: the extract is concentrated under reduced pressure to 50mL, slowly add 160mL of 95% ethanol to make the alcohol content of the extract reach 80%, store at 4°C for 12h, centrifuge, take the supernatant, and concentrate under reduced pressure to no alcohol taste;

[0047] C) Separation of macroporous resin: AB-8 type macroporous resin wet-packed column, diameter-to-height ratio 1:12, step B) obtained concentrated solution wet loading, with distilled water, volume fraction 30%, 50% and 95% ethanol solution as the el...

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Abstract

The invention provides a water-extraction preparation method and an application of an antibacterial active component in bamboo willow. Bamboo willow bark powder is subjected to ultrasonic extraction 1-5 times with distilled water diluted by 5-20 times and for 30-120 min each time, and an extraction solution is obtained; the extraction solution is subjected to alcohol precipitation to remove water-soluble impurities and then separated with macroporous resin, 95% ethanol elution sections are collected, concentrated under reduced pressure and purified with Sephadex LH-20 to remove pigments, and the antibacterial active component in the bamboo willow is obtained. The bamboo willow water extract has better inhibition activity for bacteria such as beta hemolytic streptococcus, staphylococcus aureus, bacilli, escherichia coli, pseudomonas aeruginosa and the like and can serve as a prescribed preparation to be directly used for treating diseases caused by bacterial infection, such as acute tonsillitis, acute pharyngitis, acute laryngitis and acute otitis media. With the adoption of the method, the antibacterial active component is successfully extracted from the bamboo willow and a foundation is laid for application of the bamboo willow in the field of medicine products.

Description

technical field [0001] The invention relates to a water extraction preparation method and application of bamboo willow antibacterial active components, and belongs to the field of extraction, separation and purification of effective components of natural products. Background technique [0002] Bamboo willow (Salix ssp.), also known as American bamboo willow, is a plant of the genus Salix in the Salix family (Saliaceae). Traditional Salix plants have many excellent qualities, and at the same time show the advantages of fast growth, good material quality, and strong resistance (Zhang Jian et al., 2009), and are commonly used fast-growing timber forest species and biomass energy tree species (Xu Keshun, 2011). [0003] In recent years, the planting area of ​​bamboo and willow has expanded rapidly in my country. According to statistics, its forest area has exceeded 1300hm 2 (Hu Guotao et al., 2016), bamboo and willow reserves are abundant, but the industrialization of bamboo an...

Claims

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Application Information

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IPC IPC(8): A61K36/76A61P31/04A61P11/04A61P27/16
CPCA61K36/76A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/55Y02A50/30
Inventor 何秋霞张姗姗韩利文刘可春韩拥政党立王莹张轩铭李晓彬侯海荣郭敬兰王雪王丽娟
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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