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Test strip, kit and detection method for high-sensitivity detection of group A B hemolytic streptococcus

A high-sensitivity technology for hemolytic streptococcus, which is applied in the field of test strips for the detection of group A beta-hemolytic streptococcus, can solve the problem of large influence of sampling position and sampling volume, improper mixing order or mixing ratio, tracer The problem of slow drug release speed can be solved, and the effect of shortening the interpretation time, reducing the production process and saving labor cost can be achieved.

Pending Publication Date: 2020-11-24
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. The commonly used samples are oropharyngeal swabs, and the sensitivity of the kit is generally 10 5 And above, this sensitivity kit is greatly affected by the swab sampling position and sampling volume, and it is prone to false negative results
[0006] 2. The swab needs to be pre-treated, and the two liquids need to be mixed in proportion. If the mixing sequence or mixing ratio is improper, it is easy to cause wrong results
[0007] 3. Conventional immunochromatography products, the tracer conjugate pad is pressed with 1mm film, and the sample pad is pressed with 1mm tracer pad. Such overlapping will form a water storage area, and the tracer release speed is slow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: a kind of test paper strip of highly sensitive detection group A beta-hemolytic streptococcus, said reagent strip comprises absorbent paper, bottom plate, nitrocellulose membrane and antibody-red latex microsphere marker integrated pad, said The antibody-red latex microsphere marker integrated pad is located under the nitrocellulose membrane, and the colored end of the antibody-red latex microsphere marker integrated pad is connected to the nitrocellulose membrane, and the nitrocellulose membrane is pasted on At the middle position of the bottom plate, the top of the nitrocellulose membrane is pasted with absorbent paper. The bottom plate is a PVC board. After the product obtained in the above steps is flattened, it is cut into test strips with a width of 3.0 mm.

[0046] The preparation method of the antibody-red latex microsphere marker integrated pad is as follows:

[0047] (1) Cleaning and activation of red latex microspheres

[0048] Measure red latex ...

Embodiment 2

[0056] Embodiment 2: a kind of test paper strip of highly sensitive detection group A beta-hemolytic streptococcus, said reagent strip comprises absorbent paper, base plate, nitrocellulose membrane and antibody-red latex microsphere marker integrated pad, said The antibody-red latex microsphere marker integrated pad is located under the nitrocellulose membrane, and the colored end of the antibody-red latex microsphere marker integrated pad is connected to the nitrocellulose membrane, and the nitrocellulose membrane is pasted on At the middle position of the bottom plate, the top of the nitrocellulose membrane is pasted with absorbent paper. The bottom plate is a PVC board. After the product obtained in the above steps is flattened, it is cut into test strips with a width of 3.0 mm.

[0057] The preparation method of the antibody-red latex microsphere marker integrated pad is as follows:

[0058] (1) Cleaning and activation of red latex microspheres

[0059] Measure red latex ...

Embodiment 3

[0067] Embodiment 3: a kind of test paper strip of highly sensitive detection group A beta-hemolytic streptococcus, said reagent strip comprises absorbent paper, base plate, nitrocellulose membrane and antibody-red latex microsphere marker integrated pad, said The antibody-red latex microsphere marker integrated pad is located under the nitrocellulose membrane, and the colored end of the antibody-red latex microsphere marker integrated pad is connected to the nitrocellulose membrane, and the nitrocellulose membrane is pasted on At the middle position of the bottom plate, the top of the nitrocellulose membrane is pasted with absorbent paper. The bottom plate is a PVC board. After the product obtained in the above steps is flattened, it is cut into test strips with a width of 3.0 mm.

[0068] The preparation method of the antibody-red latex microsphere marker integrated pad is as follows:

[0069] (1) Cleaning and activation of red latex microspheres

[0070] Measure red latex ...

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Abstract

The invention provides a test strip for detecting group A B hemolytic streptococcus with high sensitivity. The detection sensitivity is high, the color development speed is high, a tracer conjugate pad and a sample pad are unified into an antibody-red latex microsphere marker integrated pad by processing glass fibers, forming of a water storage area is avoided, the tracer release speed is increased, the interpretation time is shortened, and one production process is reduced, so that the labor cost is saved; the invention also provides a kit for high-sensitivity detection of group A B hemolyticstreptococcus, which is convenient to use and simple to operate, and can prevent error results caused by improper mixing sequence or mixing proportion. The invention further provides a detection method for detecting the group A B hemolytic streptococcus with high sensitivity, and the method is simple, high in operability and short in consumed time.

Description

technical field [0001] The invention relates to the technical field of detection of group A beta-hemolytic streptococci, in particular to a test strip, a kit and a detection method for detecting group A beta-hemolytic streptococci. Background technique [0002] Group A streptococcus (StrepA), also known as Streptococcus pyogenes, accounts for more than 90% of pathogenic streptococci, is the most pathogenic bacteria among streptococci, and can produce a variety of enzymes And exotoxins, can cause acute pharyngitis, respiratory tract infection, erysipelas, scarlet fever, pneumonia and allergic diseases, such as acute rheumatic fever (ARF), rheumatic heart disease (RHD), acute glomerulonephritis, etc. [0003] According to WHO data, there are about 616 million cases of acute angina caused by group A beta-hemolytic streptococcus, 111 million cases of impetigo, and 18.1 million cases of severe diseases, with 1.78 million new cases being added every year. At least 1.034 million p...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543G01N33/558
CPCG01N33/56944G01N33/54313G01N33/558G01N2333/315
Inventor 杨帆高洪元邓旭刘仁萍杨明霞
Owner 山东康华生物医疗科技股份有限公司
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