The invention provides a digital PCR (
polymerase chain reaction)
chip. The digital
PCR chip comprises a droplet production module formed by laminating a
cover glass and a glass substrate and a droplet collecting module tightly attached to one surface of the droplet production module, wherein two through holes including a
liquid phase sample inlet and an
oil phase sample inlet are formed in the
cover glass; microflow channels communicated withthe
liquid phase sample inlet or the
oil phase sample inlet are etched in one surface, attached to the glass substrate, of the
cover glass, each microflow channel is divided into two
branch channels, and the
branch channels pairwise intersect with droplet production ports; the droplet collecting module comprises a cavity with two opening sides, and the droplet production ports are all tightly attached to the inner side of one opening. The digital
PCR chip is provided with the independent droplet collecting module, the PCR and the result observation are both performed in the
chip, so that the probabilities of
pollution and demulsificationare greatly reduced, meanwhile, results of the PCR are observed under a
fluorescence microscope in a unified manner, signals reading of droplets is not required to be performed in the
chip one by one, and the
signal reading time is greatly shortened.