The invention provides a digital PCR (polymerase chain reaction) chip. The digital PCR chip comprises a droplet production module formed by laminating a cover glass and a glass substrate and a droplet collecting module tightly attached to one surface of the droplet production module, wherein two through holes including a liquid phase sample inlet and an oil phase sample inlet are formed in the cover glass; microflow channels communicated withthe liquid phase sample inlet or the oil phase sample inlet are etched in one surface, attached to the glass substrate, of the cover glass, each microflow channel is divided into two branch channels, and the branch channels pairwise intersect with droplet production ports; the droplet collecting module comprises a cavity with two opening sides, and the droplet production ports are all tightly attached to the inner side of one opening. The digital PCR chip is provided with the independent droplet collecting module, the PCR and the result observation are both performed in the chip, so that the probabilities of pollution and demulsificationare greatly reduced, meanwhile, results of the PCR are observed under a fluorescence microscope in a unified manner, signals reading of droplets is not required to be performed in the chip one by one, and the signal reading time is greatly shortened.