The invention discloses a device and method for checking blank of a cup of a biochemical analysis meter. The device consists of a reaction disc, reaction cups, a cleaning mechanism, an optical mechanism, a data acquisition system, a main control mechanism and a mechanism controller. The plurality of reaction cups are sequentially fixedly arranged around the circumference of the reaction disc, the cleaning mechanism, the optical mechanism, the data acquisition system and the main control mechanism are arranged around the reaction disc, the optical mechanism is arranged on and is connected with the data acquisition system, the data acquisition system is connected with the main control mechanism, and the mechanism controller is connected with the reaction disc and the cleaning mechanism. The method comprises the following steps of: firstly, automatically cleaning the reaction cups; secondly, obtaining blank values of the reaction cups; and thirdly judging abnormality of the blank values of the reaction cups. The device and the method for checking the blank of the cup of the biochemical analysis meter, disclosed by the invention, have the beneficial effects of fastness, accuracy, and prevention on error results in a test to obtain the accurate result.