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Lignin in dandelion, its bacteria-resisting activity and use for medicine

A technology of lignin and dandelion acid, applied in the field of medicine, can solve problems such as unclear chemical composition

Inactive Publication Date: 2007-08-29
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few domestic research reports on the chemical components of Dandelion plants, and most of the chemical components of the medicinal dandelion Taraxacum officinale are cited. The current search results found that Ling Yun’s research group has conducted a research report on the chemical components of the Mongolian dandelion T. mongolicum [Ling Yun, Bao Yanyan, Zhu Lili , Zheng Junhua, Cai Shaoqing, Xiao Yue, Research on Chemical Constituents of Dandelion Chinese Journal of Pharmaceutical Sciences, 1997, 32, 584-586; Ling Yun, Bao Yanyan, Zhang Yonglin, Cai Shaoqian, Zheng Junhua, Research on Chemical Constituents of Dandelion, Journal of Naval General Hospital, 1998, 11, 167-169], a total of 5 compounds were obtained: β-sitosterol, quercetin, caffeic acid, chlorogenic acid and luteolin-7-oxo-glucoside, while other chemical components are not yet clear

Method used

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  • Lignin in dandelion, its bacteria-resisting activity and use for medicine
  • Lignin in dandelion, its bacteria-resisting activity and use for medicine
  • Lignin in dandelion, its bacteria-resisting activity and use for medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation of Mongolian Taraxacid A and Rufescidride:

[0022] 1.1 Instruments and reagents

[0023] The melting point was measured with a microscopic melting point apparatus (produced by Beijing Tektronix Co., Ltd.), and the temperature was not corrected; the optical rotation was measured on a Polax-2L automatic polarimeter produced in Japan; the infrared spectrum IR was measured by a Bruker Vector-22 infrared spectrometer, and pressed by KBr; Spectra were measured with a Shimdzu UV-240 ultraviolet spectrophotometer; hydrogen nuclear magnetic resonance 1 H-NMR, carbon nuclear magnetic resonance spectrum 13C-NMR and 2D NMR were measured by INOVA superconducting nuclear magnetic resonance spectrometer (VARIANINOVA-400MHz) (tetramethylsilyl ether TMS was used as internal standard); (100-200, 200-300 and 300-400 mesh) and silica gel GF254 (10-40 mesh) for thin layer chromatography are all produced by Qingdao Ocean Chemical Factory; all reagents used are analyt...

Embodiment 2

[0039] Example 2: Detection of the Ability of Lignin Compounds 1 and 2 to Inhibit Gram-positive Bacteria

[0040] 2.1 Principle:

[0041] The "tube-and-disc method" is used to study the in vitro antibacterial activity of the test substance, which is to use the test drug added to the Oxford cup to diffuse into the medium inoculated with the test bacteria, thereby inhibiting the growth of the bacteria. By detecting the antibacterial activity formed around the Oxford cup The diameter of the circle was used to determine the antibacterial activity of the test drug.

[0042] 2.2 Detection of bacteria:

[0043] Staphylococcus aureus 26003-23 ​​was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0044] Beta-hemolytic Streptococcus 32210 was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0045] 2.3 Test drugs:

[0046] DMSO: DMSO solvent control;

[0047] Norfloxacin: 1.25mg / ml;

[0048] Mo...

Embodiment 3

[0063] Implementation 3: Comparison of Compounds 1 and 2 in Rhizomes and Shoots and Leaves of Five Species of Dandelion

[0064] Dandelion (Taraxacum mongolicum Hand-Mazz), Dandelion Alkaline (Taraxacum sinicum Kitag), Dandelion Jehol (Taraxacum platypecidum Diels), Dandelion Northeast (Taraxacum ohwianum Kitag), Dandelion inverse (Taraxacum grypodn Dahlst) and Xing'an dandelion (Taraxacum falcilobum Kitag) were collected and identified by Professor Peng Hua from the Kunming Institute of Botany, Chinese Academy of Sciences.

[0065] Waters high performance liquid chromatography (Waters HPLC, 2695 separation system includes quaternary pump, column thermostat autosampler; 2996 ultraviolet diode matrix detector, Empower chromatographic operation software and Symmetry  C18 chromatographic column (4.6mm×250mm), 5μm) was used to detect the content of compounds 1 and 2.

[0066] Treatment of test samples: Weigh 0.5 g (±0.1 mg) of each sample after drying in the shade, place in a ...

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Abstract

The invention relates to a lignin compound that could prevent yellow staphylococcal bacteria and B type hemolytic streptococcus, and the medicine combination of the compound. The invention of lignin Morgan dandehon herb acid A and Rufescidride has obviously effect restraining yellow staphylococcal and B type hemolytic streptococcus. It is also respected to prevent the diseases caused by them, like boil, blain, toxicity epidermis necrolysis, pneumonia, etc.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular, the present invention relates to two lignins isolated from dandelion that can prevent and treat Staphylococcus aureus and / or beta-hemolytic streptococcus infection and its pharmaceutically acceptable salt, and its Anti-infective drug use, and pharmaceutical compositions containing these compounds. It is expected to be used in the prevention and treatment of boils, pustules, toxic epidermal decomposition, pneumonia, osteomyelitis, acute myocarditis, endocarditis, meningitis, mastitis caused by Staphylococcus aureus and / or beta-hemolytic streptococcal infection , cystitis, pelvic inflammatory disease, urinary tract inflammation, prostatitis, bacteremia, toxic shock syndrome, lymphadenitis, arthritis, pharyngitis, cervicitis, and abscesses of muscles, skin, urogenital tract, and central nervous system . technical background [0002] Staphylococcus aureus, a Gram-positive co...

Claims

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Application Information

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IPC IPC(8): C07D311/78A61K31/352A61K36/288A61P31/04
Inventor 李校堃施树云徐艳陶巧凤约阿施·史托克希特赵昱
Owner WENZHOU MEDICAL UNIV
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