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Two flavone glycosides in dandelion and medical use for against Gram-positive bacterium thereof

A technology of dandelion glycosides and dandelion, which is applied in the field of medicine and can solve problems such as unclear chemical components

Active Publication Date: 2007-08-15
DALI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few domestic research reports on the chemical constituents of Dandelion plants, and the chemical constituents of the medicinal dandelion Taraxacum officinale are mostly cited. The current search results found that Ling Yun’s research group has researched and reported on the chemical constituents of Mongolian Dandelion [Ling Yun, Bao Yanyan, Zhu Lili, Zheng Junhua, Cai Shaoqing, Xiao Yue, Research on Chemical Constituents of Dandelion, Chinese Journal of Pharmaceutical Sciences, 1997, 32, 584-586; Ling Yun, Bao Yanyan, Zhang Yonglin, Cai Shaoqing, Zheng Junhua, Research on Chemical Constituents of Dandelion, Journal of Naval General Hospital, 1998, 11, 167- 169], a total of 5 compounds were obtained: β-sitosterol, quercetin, caffeic acid, chlorogenic acid and luteolin-7-oxo-glucoside, while other chemical components are not yet clear

Method used

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  • Two flavone glycosides in dandelion and medical use for against Gram-positive bacterium thereof
  • Two flavone glycosides in dandelion and medical use for against Gram-positive bacterium thereof
  • Two flavone glycosides in dandelion and medical use for against Gram-positive bacterium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Separation of Mongolian Taraxacin A and Mongolian Taraxacin B:

[0024] 1.1 Instruments and reagents

[0025]The melting point was measured with a micro melting point apparatus (produced by Beijing Tektronix Co., Ltd.), and the temperature was not corrected; the optical rotation was measured on a Polax-2L automatic polarimeter produced in Japan; the infrared spectrum (IR) was measured by a Bruker Vector-22 infrared spectrometer, and the KBr tablet ; Ultraviolet spectrum is measured with Shimadzu UV-240 ultraviolet spectrophotometer; Proton nuclear magnetic resonance spectrum ( 1 H-NMR), carbon nuclear magnetic resonance spectrum ( 13 C-NMR) and two-dimensional nuclear magnetic resonance (2D NMR) were determined by INOVA superconducting nuclear magnetic resonance spectrometer (VARIAN INOVA-400 MHz) (tetramethylsilyl ether TMS as internal standard); electrospray mass spectrometry (ESI-MS ) was measured by Bruker Esquire 3000+ mass spectrometer, silica gel GF...

Embodiment 2

[0048] Example 2: Detection of the Ability of Flavonoid Glycosides 1 and 2 to Inhibit Gram-positive Bacteria

[0049] 2.1 Principle:

[0050] The "tube-and-disk method" is used to study the in vitro antibacterial activity of the test drug, which is to use the test drug added in the Oxford cup to diffuse into the medium inoculated with the test bacteria, thereby inhibiting the growth of the bacteria. The diameter of the bacterial circle was used to determine the antibacterial activity of the test drug.

[0051] 2.2 Detection of bacteria:

[0052] Staphylococcus aureus 26003-23 ​​was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0053] Beta-hemolytic Streptococcus 32210 was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0054] 2.3 Test drugs:

[0055] DMSO: DMSO solvent control;

[0056] Norfloxacin: 1.25mg / ml;

[0057] Mongolian taraxacin A (compound 1): 5.0mg / ml; Mongolian taraxa...

Embodiment 3

[0074] Example 3: Comparison of Compounds 1 and 2 in Rhizomes and Shoots and Leaves of Five Species of Dandelion

[0075] Dandelion (Mongolian dandelion) (Taraxacum mongolicum Hand-Mazz), Alkaline dandelion (Hua dandelion) (Taraxacum sinicum Kitag), Jehol dandelion (White margin dandelion) (Taraxacum platypecidum Diels), Northeast dandelion (Taraxacum ohwianum Kitag), anti-bract dandelion (Taraxacum grypodon Dahlst) and Xing'an dandelion (Taraxacum falcilobum Kitag) each 20 grams were collected and identified by Professor Peng Hua from Kunming Institute of Botany, Chinese Academy of Sciences.

[0076] Waters high performance liquid chromatography (Waters HPLC, 2695 separation system comprises quaternary pump, column thermostat autosampler; 2996 ultraviolet diode matrix detector, Empower chromatographic operation software and Waters Symmetry  RP-18 4.6mm×250mm, 5μm chromatographic column) was used to detect the content of compounds 1 and 2.

[0077] Treatment of test sample...

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Abstract

The invention discloses a flavonoid glycoside and medical salt and application to prevent Micrococcus pyogenes and beta hemolytic streptococcus, which is characterized by the following: preventing pustule, toxic epidermal decay, acute myocarditis, cystitis, cervicitis and tumour of neutral nerve system; fitting for treating prostatitis, pneumococcus, medullitis and knuckle disease.

Description

technical field [0001] The invention relates to the technical field of medicine. Specifically, the present invention relates to two flavonoid glycosides with novel structures and pharmaceutically acceptable salts thereof, and their anti-infective drug use isolated from Dandelion , and pharmaceutical compositions containing these compounds. It is expected to be used in the prevention and treatment of boils, pustules, toxic epidermal decomposition, pneumonia, osteomyelitis, acute myocarditis, endocarditis, meningitis, mastitis caused by Staphylococcus aureus and / or beta-hemolytic streptococcal infection , cystitis, pelvic inflammatory disease, urinary tract inflammation, prostatitis, bacteremia, toxic shock syndrome, lymphadenitis, arthritis, pharyngitis, cervicitis, and abscesses of muscles, skin, urogenital tract, and central nervous system . technical background [0002] Staphylococcus aureus, a Gram-positive coccus, and methylcillin-resistant Staphylococcus aureus (MRSA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61K36/288A61P31/04
Inventor 李峰施树云徐艳陶巧凤关静宋月晗曾苏赵昱
Owner DALI PHARMA
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