Method for rapidly extracting egg yolk antibody, oral ulcer-resistant product prepared thereby and application thereof

Abstract

The invention discloses a method for rapidly extracting an egg yolk antibody, an oral ulcer-resistant product prepared thereby and application thereof. The method comprises the following steps: immunizing laying hens by beta hemolytic streptococcus, escherichia coli and candida albicans all-bacteria vaccine; mechanically automatically separating eggs to obtain yolk; diluting a citric acid-disodiumhydrogen phosphate buffer solution and stabilizing the pH value of yolk liquid; adding dry ice, rapidly reducing the system temperature, performing continuous high-speed low-temperature centrifugation and removing fat particles to obtain yolk antibody supernatant; enabling the yolk antibody supernatant to pass through double-layer filter membranes of 2 to 6 um and 0.3 to 0.8 um and a hollow fiberultrafiltration membrane with molecular weight cut-off of 300 kD to remove impure protein as well as pass through a hollow fiber ultrafiltration membrane with molecular weight cut-off of 100 kD to obtain concentrated yolk antibody liquid; and adding auxiliary materials to prepare oral mucosal spray. The method has the advantages of high automation degree, low energy consumption, high yield, environmental friendliness, capability of realizing large-scale industrialized production and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, especially the biotechnology of obtaining antibodies through immunization and breeding technology and preparing them into medicines or health care products, and particularly relates to a rapid extraction method of egg yolk antibodies, prepared anti-oral ulcer products and applications thereof. Background technique [0002] Recurrent aphthous ulcer (RAU), also known as recurrent aphthous stomatitis (RAS), is a common ulcerative inflammatory disease in the oral cavity, which occurs in childhood or adolescence, with periodic, recurrent. The lesions appear as round or oval superficial ulcers, with clear erythematous edges, such as halos, and the central depression is yellow or gray. The patient has obvious burning pain, so it is named "Aphthus" in Greek — Burning pain. Immunity, heredity and environment are triple factors for the pathogenesis of oral ulcers, including the invasion of viruses an...

AI Technical Summary

Problems solved by technology

For example: collecting egg yolk by hand-beating eggs is inefficient and not suitable for large-scale use, and it is easy to introduce pollution sources, increasing the risk of contamination of egg yolk antibodies; using repeated freezing and thawing 1-3 times, if used The production operation is time-consuming and laborious; use organic solvents such as caprylic acid or chloroform to extract IgY, the application of organic solvents affects the activity of IgY, and is toxic, pollutes the environment, and is inconvenient to operate; adopts ammonium sulfate salting out precipitation method, there are centrifugation to collect precipitation and Time-consuming operations such as reconstitution and precipitation; the water dilution extraction method is used to extract IgY, although it is suitable for large-scale production and protection of IgY activity, but the extraction time is prolonged. Lowered to 0-8°C, the production process is time-consuming and energy-consuming in actual production, and the efficiency of large-scale industrial production is low

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