Black purple Tuowusu and medical application in restraining grampostive bacteria

A Gram-positive, black-purple technology, applied in the field of medicine, can solve the problems of chemical composition research and biological activity research that are still rarely reported.

Inactive Publication Date: 2007-10-10
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the chemical c...

Method used

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  • Black purple Tuowusu and medical application in restraining grampostive bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation of black purple Arghine A

[0018] 1.1 Instruments and reagents

[0019] The melting point was measured with a micro-melting point apparatus (produced by Beijing Tektronix Co., Ltd.), and the temperature was not corrected; the optical rotation was measured on a Polax-2L automatic polarimeter produced in Japan; the infrared spectrum (IR) was measured by a BrukerVector-22 infrared spectrometer, and pressed by KBr; The ultraviolet spectrum was measured with a Shimadzu UV-240 ultraviolet spectrophotometer; the hydrogen nuclear magnetic resonance spectrum ( 1 H-NMR), carbon nuclear magnetic resonance spectrum ( 13 C-NMR) and 2D NMR were measured by INOVA type superconducting nuclear magnetic resonance spectrometer (VARIAN INOVA-400MHz) (tetramethylsilyl ether was internal standard); electrospray mass spectrometry (ESI-MS) was measured by Bruker Esquire3000+ mass spectrometer, column Silica gel for chromatography (100-200, 200-300, 300-400 mesh...

Embodiment 2

[0029] Embodiment 2: detection of the ability of the compound of formula (1) to inhibit Gram-positive bacteria

[0030] 2.1 Principle:

[0031] The "tube-and-disk method" is used to study the in vitro antibacterial activity of the test drug, which is to use the test drug added in the Oxford cup to diffuse into the medium inoculated with the test bacteria, thereby inhibiting the growth of the bacteria. The diameter of the bacterial circle was used to determine the antibacterial activity of the test drug.

[0032] 2.2 Detection of bacteria:

[0033] Staphylococcus aureus 26003-23 ​​was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0034] Beta-hemolytic Streptococcus 32210 was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0035] 2.3 Test drugs:

[0036] DMSO: DMSO solvent control;

[0037] Norfloxacin: 1.25mg / ml;

[0038] Compound of formula (1): 5.0 mg / ml; the sample was dissolv...

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Abstract

This invention relates to Ligulatrovine A separated from Ligularia actroviolacea that can prevent and treat diseases related to Staphylococcus aureus and beta hemolytic streptococcus, its pharmaceutical salts and drug composition. Ligulatrovine A has significant inhibitive effect on Gram-positive bacteria, and can prevent and treat diseases such as boil, pustule, pneumonia, osteomyelitis, acute myocarditis, endocarditis, meningitis, mastitis, cystitis, pelvic inflammation, urinary inflammation, prostatitis, bacteremia, or abscess in muscle, skin, urogential region or central nervous system caused by Gram-positive bacteria infection.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular, the present invention relates to a trans-butene black ligusin A and Its pharmaceutically acceptable salts and pharmaceutical compositions. The sesquiterpene has the effect of obviously inhibiting the growth of Staphylococcus aureus and beta-hemolytic streptococcus, and can be expected to be used to prevent boils, pustules, pneumonia, osteomyelitis, acute myocarditis, and endocarditis caused by these two infections , meningitis, mastitis, cystitis, pelvic inflammatory disease, urinary tract inflammation, prostatitis, bacteremia, toxic shock syndrome, lymphadenitis, arthritis, pharyngitis, cervicitis, and muscle, skin, urogenital , Central nervous system abscess. technical background [0002] Staphylococcus aureus (Staphylococcus aureus, referred to as "Staphylococcus aureus") is a Gram-positive coccus, and methylcillin-resistant Staphylococcus aureus (MRSA) is a strain th...

Claims

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Application Information

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IPC IPC(8): C07C65/24A61K31/192A61P31/04A61K36/28
Inventor 黄可新赵军施树云马建设王彩芳王晓雨董南赵昱
Owner WENZHOU MEDICAL UNIV
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