Industrialized breeding method for sterile cordyceps sinensis host larvae

A technology for host and larvae of Cordyceps sinensis, which is applied in the field of industrialized breeding of sterile Cordyceps sinensis host larvae, can solve the problems of limited infection rate of Cordyceps sinensis host larvae, high mortality rate of Cordyceps sinensis host larvae, inability to achieve high yield of artificially cultivated Cordyceps sinensis, etc. The effect of survival

Active Publication Date: 2021-10-01
重庆欣时达生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the industrialization of artificial cultivation of Cordyceps sinensis, there are many pathogenic microorganisms, which lead to a high mortality rate of the host larvae of Cordyceps sinensis, and the competition of other microorganisms also leads to the limitation of the infection rate of the host larvae of Cordyceps sinensis, and it is impossible to realize the high yield of artificially cultivated Cordyceps sinensis. Therefore, sterile Cordyceps sinensis Industrial breeding of host larvae is particularly important

Method used

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  • Industrialized breeding method for sterile cordyceps sinensis host larvae
  • Industrialized breeding method for sterile cordyceps sinensis host larvae
  • Industrialized breeding method for sterile cordyceps sinensis host larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] An industrialized feeding method for sterile Cordyceps sinensis host larvae. After preparing sterile eggs, sterile feed, and sterile feeding devices prepared in advance, they are placed in a sterile room, and after no pollution is detected, the door in the sterile room is opened. Fan, open the aseptic feeding device, transfer the prepared aseptic feed to the aseptic device with aseptic tools, place the sterile eggs on the aseptic feed, fasten the top cover of the aseptic device, and place it in the aseptic production plant area Inside.

[0029] The preparation of the above-mentioned sterile eggs includes placing the mating eggs that are expected to hatch in three days in a sterile egg disinfection device, immersing them in a chlorine-containing disinfectant with a concentration of 100-300ppm for 5-20 minutes, taking them out, and rinsing them with sterile water. Disinfectant on the surface of worm eggs to obtain sterile worm eggs;

[0030] Such as figure 1 The aseptic...

Embodiment 2

[0041] The rest of the method steps are the same as in Example 1, only the aseptic feed of the following formula is added in the sterile feeding bottle, formula 2: agar 1-1.5 g / 100 ml water, carrot powder 3-5 g / 100 ml water, fern powder 2.5-3.5g / 100ml water, Ph6.0-6.5. After multiple cycles of feeding, observe the growth of the larvae.

Embodiment 3

[0043] The rest of the method steps are the same as in Example 1, only the aseptic feed of the following formula is added in the aseptic rearing bottle, formula 3: agar 1-1.5 g / 100 ml water, carrot powder 3-5 g / 100 ml water, Polygonum versicolor powder 1.5-2.5g / 100ml water, Ph6.0-6.5. After multiple cycles of feeding, observe the growth of the larvae.

[0044] The larvae raised according to the method of the present invention are ground and cultivated on the culture medium after the whole worm is ground, and then the high-nutrition bacteria and fungal culture medium of various formulas are carried out to detect the sterility of the aseptic larvae. The results are as follows: Figure 4-5 As shown, it can be seen from the results that fungi and bacteria did not grow in the culture medium, and thus the results of the culture of sterile worms were in line with expectations.

[0045] Bacterial medium formula control group 1: 1. Agar 1.5g / 100ml water + Cordyceps sinensis bat moth l...

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Abstract

The invention discloses an industrialized breeding method for sterile cordyceps sinensis host larvae. The industrialized breeding method comprises the following steps of preparing sterile eggs, sterile feed and a sterile breeding device which are prepared in advance, placing the sterile eggs, the sterile feed and the sterile breeding device in a sterile room, detecting no pollution, then turning on a fan, turning on the sterile breeding device, moving the prepared sterile feed into the sterile device by using a sterile tool, placing the sterile eggs on the sterile feed, fastening a top cover of the sterile device, and placing in a sterile production plant area. The industrialized breeding method has the advantages that sterile insects are bred, no outside pathogenic microorganism enters, the survival rate can be increased, and the sterile insects lack horizontally transmitted microorganisms and are weaker in immunocompetence, so that infection of cordyceps sinensis can be facilitated, the method can provide a professional platform for researching the health relationship between microorganisms and the cordyceps sinensis host larvae, including pathogen research of some diseases, antagonism among microorganisms, virus disease research, bacteriology research, fungal infection research and protozoa infection research.

Description

technical field [0001] The invention relates to the field of artificial cultivation of Cordyceps sinensis, in particular to an industrialized feeding method for aseptic Cordyceps sinensis host larvae. Background technique [0002] Cordyceps sinensis, the name of the traditional Chinese medicine, is a complex of the larvae of the larvae and the larvae of the ergotaceae plant Cordyceps sinensis. Cordyceps sinensis has many functions such as regulating the function of the immune system, anti-tumor, anti-fatigue, nourishing the lung and kidney, stopping bleeding and reducing phlegm, secreting essence and nourishing qi. In the industrialization of artificial cultivation of Cordyceps sinensis, there are many pathogenic microorganisms, which lead to a high mortality rate of the host larvae of Cordyceps sinensis. The competition of other microorganisms also leads to a limitation of the infection rate of the host larvae of Cordyceps sinensis, and the high yield of artificially cultiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/30A01G18/00A01G18/20
CPCA01K67/033A23K50/90A23K10/30A01G18/00A01G18/20
Inventor 柴文敏毛先兵卜俊熙卜京
Owner 重庆欣时达生物技术有限公司
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