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A kind of preparation method of Clostridium perfringens type A inactivated vaccine of Clostridium welchii disease in rabbits

A technology for Clostridium perfringens and Clostridium welchii disease, which is applied to bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of unsatisfactory immune protection rate and safety, and is convenient for large-scale production and operation. Simple process and low cost effect

Active Publication Date: 2017-03-01
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immune protection rate and safety of these vaccines to animals are not ideal, so we urgently need new biological agents with good immune effect, safety and reliability, and then apply them to the control of this disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of preparation method of the Clostridium perfringens type A inactivated vaccine of rabbit clostridium welchii disease, concrete steps are as follows:

[0029] (1). Preparation of strains for production

[0030] Inoculate the standard strain of Clostridium perfringens type A NCTC528 on a blood agar plate, and culture it in an anaerobic box at 37°C for 36 hours to recover; pick typical colonies and inoculate them on 7ml of nutrient broth medium and inoculate them anaerobically at 39°C for 12 hours As a primary seed culture solution; then inoculate 2ml of the primary seed culture solution in 100ml nutrient broth medium for anaerobic culture at 39°C for 12h as a secondary seed culture solution;

[0031] (2). Preparation of bacterial solution for seedling production

[0032] Put 70ml of the secondary seed culture solution obtained above into 1L of toxin-producing medium, and culture it under anaerobic environment at 45°C with stirring at 200r / min for 4h;

[0033] (3...

Embodiment 2

[0042] Vaccine efficacy test, the specific method is as follows:

[0043]10 healthy rabbits of 1.5-2.0kg, 5 of which were subcutaneously injected with 2ml of the vaccine in Example 1, and the other 5 were used as a contrast. After 21 days, all of them were intravenously injected with a lethal dose of Clostridium perfringens type A toxin, and observed for 10 days , all died in the control group, and all survived in the immunized group, which proved that the protection rate of the inactivated vaccine to rabbits reached 100%.

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PUM

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Abstract

The invention relates to the field of animal bacteriology and provides a preparation method of an inactivated vaccine of A-type clostridium perfringens for clostridium welchii disease of a domestic rabbit. The preparation method comprises the following steps: adopting the A-type clostridium perfringens as an original strain, finally obtaining a bacterium solution by primary and secondary culture, mixing the bacterium solution and white oil adjuvant and preparing the inactivated vaccine of the A-type clostridium perfringens. The vaccine prepared by the method is immunized for the domestic rabbit, the protective rate reaches 100% after counteracting toxic substances, so that good immunoprotection action is achieved.

Description

technical field [0001] The invention relates to the field of animal bacteriology and provides a method for preparing an inactivated vaccine of clostridium perfringens type A of rabbit clostridium welchii disease. Background technique [0002] Clostridium welchii disease This disease is also called Clostridium perfringens disease. Clostridium welchii enteritis is mainly caused by the alpha toxin produced by Clostridium perfringens type A. It widely exists in soil, feed, vegetables, sewage, and feces. The occurrence of this disease has no obvious seasonality, and rabbits of various ages, breeds and sexes are susceptible except for suckling young rabbits. The main infectious agents of this disease are sick rabbits, bacteria-carrying rabbits and their excrement. Pathogens invade the body from the digestive tract or wounds, reproduce in large numbers on the chorion of the small intestine and cecum, and produce strong α toxins, which change the permeability of capillaries and a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/114A61P31/04C12N1/20
Inventor 柴同杰郭梦娇王海荣
Owner SHANDONG AGRICULTURAL UNIVERSITY
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