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Development and application of Nisin solid stabilizer

A stable solid and wet solid technology, applied in the fields of application, peptide, organic chemistry, etc., can solve the problem of high production cost, achieve the effect of long stability, good repeatability and low production cost

Active Publication Date: 2019-04-16
ZHEJIANG SILVER ELEPHANT BIO ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Production costs of microemulsion preparations, nanoparticle preparations, bioadhesive particles, etc. are relatively high

Method used

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  • Development and application of Nisin solid stabilizer
  • Development and application of Nisin solid stabilizer
  • Development and application of Nisin solid stabilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 3 tons of Nisin fermentation broth, sterilized by ceramic membrane filtration, the obtained supernatant was concentrated 10 times with an ultrafiltration membrane greater than 1000 Daltons, 60kg of NaCl was added to the 300L concentrated solution for salting out, and 80kg was obtained by plate and frame pressure filtration of wet solids. Take 1g, 2g, 5g, and 10g of Nisin plate and frame filter wet solids respectively, and measure the content of Nisin. The determination methods are all determined by the biological potency method, and the operation is performed according to the national standard NY / T392-2000. The results are shown in Table 1.

[0025] Table 1 The content of Nisin in the wet solid of Nisin plate and frame press (mg / g)

[0026]

[0027] The results in Table 1 show that the content of Nisin in the Nisin plate and frame filter wet solid is about 250mg / g.

Embodiment 2

[0029] Accurately weigh 1.5kg Nisin plate and frame press filter wet powder and 1.0kg NaCl, add 0.1%, 0.5%, 1%, 2%, 3% glycerol glucoside, dissolve in 10.0L aqueous solution, stir at room temperature for 15min, then add concentrated Hydrochloric acid to adjust the pH to 2.9, after stirring evenly. Take 5L of Nisin solution for spray drying of Nisin products, the inlet temperature is 180°C, the outlet temperature is 80°C, and blank control test is carried out at the same time.

[0030] In addition, 5L of Nisin solution was taken to vacuum-dry Nisin products. During the vacuum drying process, keep at 40°C for 4h and at 55°C for 4h to obtain a dry sample, which was ground and pulverized for content determination. The product obtained by spray drying and vacuum drying was subjected to a storage test at 55° C., and sampling and analysis were carried out at 7 days and 14 days respectively. The content of Nisin was determined by the biological potency method, and the determination m...

Embodiment 3

[0038] Accurately weigh 1.5kg Nisin plate and frame press filter wet powder and 1.0kg NaCl, add 0.1%, 1%, 2% low-ester pectin, methionine and chitosan, dissolve in 10.0L aqueous solution, stir at room temperature 15min, then add concentrated hydrochloric acid to adjust the pH to 2.9, and stir well. Take 5L of Nisin solution for spray drying of Nisin products, the inlet temperature is 180°C, the outlet temperature is 80°C, and blank control test is carried out at the same time.

[0039] In addition, 5L of Nisin solution was taken to vacuum-dry Nisin products. During the vacuum drying process, keep at 40°C for 4h and at 55°C for 4h to obtain a dry sample, which was ground and pulverized for content determination. The product obtained by spray drying and vacuum drying was subjected to a storage test at 55° C., and sampling and analysis were carried out at 7 days and 14 days respectively. The content of Nisin was determined by the biological potency method, and the determination ...

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Abstract

The invention provides a long-acting Nisin solid stabilizer, and belongs to the technical field of microbial preservation. Every 100g of the stabilizer includes the following raw materials: 0-60g of Nisin plate frame filter press wet powder; 0-40g of NaCl; 0.1-2g of glycerol glucoside. The pH is adjusted to 2.5-3.5 with concentrated hydrochloric acid. The stabilizer has high cost performance, canbe used for maintaining activity and long-term effects of Nisin solids in the fields of food, cosmetics and the like, and can be used in the Nisin production process to reduce the damage to Nisin in the spray drying and vacuum drying process.

Description

technical field [0001] The invention relates to the technical field of microorganism preservation, in particular to the preparation and application of a Nisin solid stabilizer. Background technique [0002] Nisin, also known as nisin or transliterated as Nisin, is synthesized and secreted by certain Streptococcus lactate and Lactococcus lactis in the metabolic process and has a strong bactericidal effect. The active small peptide consists of 34 amino acid residues with a molecular weight of about 3400Da. Because nisin can inhibit most Gram-positive bacteria and has a strong inhibitory effect on Bacillus spores, it is widely used in the food industry as a food preservative. . [0003] In industrial production, the preparation of Nisin solid should be vacuum-dried or spray-dried to obtain a light brown solid powder, then stored for a long time, and Nisin dissolved in water or liquid when used. During the production and storage process of Nisin solid, it is often easy to deg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/315C07K1/00A23L3/3526A23L3/3562A23L3/358
CPCA23L3/3526A23L3/3562A23L3/358C07K14/315
Inventor 王子琦张栋陈小龙陆跃乐金陈斌李光进洪超群陈艺强
Owner ZHEJIANG SILVER ELEPHANT BIO ENG
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