Kudzuvine flower isoflavone, monomer tectoridin and tectorigenin extraction method and uses

An extraction method, irisinin technology, which is applied in the field of plant medicinal component extraction, can solve the problem of no protective effect on ischemic myocardium, and achieve the effect of reducing drug cost, simple extraction process, and low cost

Active Publication Date: 2008-09-03
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it has a protective effect on ischemic myocardium is still a blank at home and abroad.

Method used

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  • Kudzuvine flower isoflavone, monomer tectoridin and tectorigenin extraction method and uses
  • Kudzuvine flower isoflavone, monomer tectoridin and tectorigenin extraction method and uses
  • Kudzuvine flower isoflavone, monomer tectoridin and tectorigenin extraction method and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Separation and Extraction of Pueraria Isoflavones

[0025] ①After crushing Pueraria lobata, put it into a Soxhlet extractor;

[0026] ② Reflux extraction with 90% ethanol for 20 hours, heat and volatilize in a water bath to obtain the total extract, dry it in a water bath for later use;

[0027] ③Suspend the ethanol crude extract of Pueraria japonica in deionized water, apply the wet method to a 50-fold volume (W:V) macroporous resin AB8 column, and elute sequentially with water, 20%, 50%, and absolute ethanol respectively. Collect the 50% ethanol eluate, concentrate under reduced pressure to recover the solvent, and obtain a mixture that is kudzu flower isoflavones.

[0028] ④ Dissolve pueraria isoflavones with 40% propylene glycol, adjust the pH value to 7.0 with 5% sodium bicarbonate, and the solubility is 10 mg / ml, and store for later use.

Embodiment 2

[0030] Separation and extraction of irisin monomer and irisin

[0031] ①Suspend the pueraria isoflavones obtained in Example 1 with deionized water, and load the sample on a polyamide column by wet method, with a weight-to-volume ratio of 1:50;

[0032] ② Wash the column with gradients of water, 20%, 30%, 40%, 50%, and absolute ethanol respectively;

[0033] ③ Collect the lotion, and recover the solvent under reduced pressure; the 30% ethanol eluate is irisin monomer, and the 50% ethanol eluate is irisin. Its structure was identified by mass spectrum, hydrogen spectrum, carbon spectrum and ultraviolet spectrum.

[0034] ④Dissolve pueraria isoflavones with 40% propylene glycol, adjust the pH value to 7.0 with 5% sodium bicarbonate, and the solubility is 10mg / ml.

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Abstract

The invention relates to a separating extraction method of isoflavone and integral iris glycosides and iris glycosides unit. It adds the ion-free water suspension into the pueravia flower alcohol course extracting matter and macro reticular resin column, the alcohol gradient elution, it collects 50% alcohol eluent and reduces the pressure to reclaim the alcohol to obtain the pueravia flower isoflavone. It adds ion-free water suspension and the polyamide column into the pueravia flower isoflavone, the alcohol gradient elution, it separately collects the alcohol eluent and reduces the pressure to reclaim the alcohol to obtain integral iris glycosides and iris glycosides unit.

Description

technical field [0001] The invention belongs to a method for extracting medicinal components of plants. In particular, it relates to a method for extracting effective components from the traditional Chinese medicine kudzu flower, which have obvious preventive and therapeutic effects on cardiovascular and cerebrovascular diseases. Background technique [0002] Isoflavones (isoflavoen) is a kind of phytoestrogen (phytoestrongen), has estrogen-like or anti-estrogen effect, is called a natural selective estrogen receptor modulator, and plays an important protective role in human health. Supplementing isoflavones can not only reduce the incidence of coronary heart disease, but also has no negative effects of hormone replacement therapy, and can also regulate human endocrine, lower blood lipids, prevent and treat menopausal syndrome, osteoporosis and anti-cancer effects. More and more studies have found that isoflavones have various regulatory effects on cardiovascular and cerebr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36C07H17/07C07H1/08
Inventor 李玉新王金凤孟祥颖鲍永利乌垠薄华本
Owner NORTHEAST NORMAL UNIVERSITY
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