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Process for preparing iris aglycone and use thereof in preparation of medicament for treating atherosclerosis

A preparation process and technology of irisin, which is applied in the field of extracting active ingredients of traditional Chinese medicines, can solve the problems of restricting the development and application of isoflavone drugs, cumbersome separation and purification procedures of isoflavones, increasing the cost of drugs, etc. Selective Unique, Large Specific Gravity Effects

Inactive Publication Date: 2009-09-30
中国人民解放军第二〇八医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the separation and purification process of isoflavones is cumbersome, time-consuming and laborious, and often requires a variety of toxic organic solvents, which not only increases the cost of drugs, but also brings more or less pollution to the environment, which limits the isoflavones to a certain extent. Development and application of flavonoids

Method used

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  • Process for preparing iris aglycone and use thereof in preparation of medicament for treating atherosclerosis
  • Process for preparing iris aglycone and use thereof in preparation of medicament for treating atherosclerosis

Examples

Experimental program
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Effect test

Embodiment 1

[0028] After crushing 10 kg of kudzu flower, add 100 kg of 70% ethanol to soak at room temperature for 7 days, extract 3 times in total, recover ethanol under reduced pressure, and obtain kudzu flower ethanol primary extract, and dry it in a water bath for later use.

[0029] Add deionized water to suspend the ethanol crude extract of Pueraria japonica, load it on the AB-8 macroporous resin column by wet method, and elute with 20% and 30% ethanol respectively. Collect the 30% ethanol eluate, concentrate under reduced pressure to recover the solvent, and obtain the mixture which is the crude irisin

[0030] The crude irisin was dissolved in 75% ethanol to a concentration of 10 mg / ml. Add 0.2mol / L hydrochloric acid, bathe in water at 80°C for 30 hours, and add sodium hydroxide to adjust the pH value to 7.0.

[0031] Recover ethanol under reduced pressure, add deionized water to suspend, and extract with n-butanol. Recover n-butanol under reduced pressure to obtain 0.12 kg of i...

Embodiment 2

[0033] After crushing 10 kg of kudzu flower, add 130 kg of 70% ethanol to soak at room temperature for 6 days, extract 3 times in total, recover ethanol under reduced pressure, and obtain kudzu flower ethanol primary extract, and dry it in a water bath for later use.

[0034] Add deionized water to suspend the ethanol crude extract of Pueraria japonica, load it on the AB-8 macroporous resin column by wet method, and elute with 20% and 30% ethanol respectively. Collect the 30% ethanol eluate, concentrate under reduced pressure to recover the solvent, and obtain the mixture which is the crude irisin

[0035] The crude irisin was dissolved in 75% ethanol to a concentration of 10 mg / ml. Add 0.2mol / L hydrochloric acid, bathe in water at 80°C for 30 hours, and add sodium hydroxide to adjust the pH value to 7.0.

[0036] Recover ethanol under reduced pressure, add deionized water to suspend, and extract with n-butanol. Recover n-butanol under reduced pressure to obtain 0.12 kg of i...

Embodiment 3

[0038] After crushing 10 kg of kudzu flower, add 150 kg of 70% ethanol to soak at room temperature for 5 days, extract twice, recover ethanol under reduced pressure, and obtain kudzu flower ethanol primary extract, and dry it in a water bath for later use.

[0039] Add deionized water to suspend the ethanol crude extract of Pueraria japonica, load it on the AB-8 macroporous resin column by wet method, and elute with 20% and 30% ethanol respectively. Collect the 30% ethanol eluate, concentrate under reduced pressure to recover the solvent, and obtain the mixture which is the crude irisin

[0040] The crude irisin was dissolved in 75% ethanol to a concentration of 10 mg / ml. Add 0.2mol / L hydrochloric acid, bathe in water at 80°C for 30 hours, and add sodium hydroxide to adjust the pH value to 7.0.

[0041] Recover ethanol under reduced pressure, add deionized water to suspend, and extract with n-butanol. Recover n-butanol under reduced pressure to obtain 0.12 kg of iridinin mon...

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Abstract

The invention discloses a new medicinal application of irisinin, which can be prepared into any conventional medicament according to the conventional pharmaceutical technology, and is used for preparing anti-atherosclerotic drugs. The invention also discloses a new process for preparing irisin, which uses ethanol and macroporous resin to separate irisin monomer from Pueraria japonica, does not use toxic organic solvents, does not pollute the environment, and meets the requirement of environmental protection. Ethanol and macroporous resin can be reused, which reduces the cost of drugs. Compared with silica gel columns, it has more sample loading, less time-consuming and high efficiency, and is suitable for industrial production.

Description

technical field [0001] The invention provides a preparation process of irisin, and also discloses its use in the preparation of anti-atherosclerotic drugs, which belongs to the technical field of extraction and pharmacy of effective components of traditional Chinese medicines. Background technique [0002] Isoflavones (isoflavoen) is a kind of phytoestrogen (phytoestrongen), has estrogen-like or anti-estrogen effect, known as natural selective estrogen receptor modulator, plays an important protective role in human health. Isoflavones have few side effects, are safe and reliable, have various biological activities, and have broad application prospects. However, the separation and purification process of isoflavones is cumbersome, time-consuming and laborious, and often requires a variety of toxic organic solvents, which not only increases the cost of drugs, but also brings more or less pollution to the environment, which limits the isoflavones to a certain extent. Developme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K36/48C07D311/36A61P9/10
Inventor 王金凤候明晓
Owner 中国人民解放军第二〇八医院
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