Rapid parallel nucleic acid detection method and system based on micro-fluidic chip

Abstract

The invention relates to a rapid parallel nucleic acid detection method and system based on a micro-fluidic chip. The nucleic acid detection system comprises a micro-fluidic chip, a motor, exciting light, a double-focal-plane imaging lens set, a detector, a signal acquisition processor and a display; the micro-fluidic chip comprises at least one reaction channel; a heating film is arranged at the periphery of the micro-fluidic chip; a submillimeter air layer is maintained between the micro-fluidic chip and the heating film; and the micro-fluidic chip is irradiated by adopting the exciting light, so that a nucleic acid sample generates fluorescence under excitation of the exciting light, the fluorescence is gathered on the detector by virtue of the double-focal-plane imaging lens set so as to generate an analog signal, the detector transmits the generated analog signal to the signal acquisition processor so as to generate a real-time fluorescence detection signal, and the real-time fluorescence detection signal is displayed by the display. The method and the system disclosed by the invention can be applied to the fields of clinical pathogenic bacterium molecular diagnosis, food inspection and quarantine, food poisoning pathogenic bacterium detection, bacteriology classification and epidemiological investigation and have huge economical and social benefits.

Description

technical field [0001] The invention relates to a nucleic acid detection method and system, in particular to a microfluidic chip nucleic acid rapid parallel detection method and system. Background technique [0002] Micro-separation in Miniaturized Total Analysis System (μTAS: Micro-separation in Miniaturized Total Analysis System) is an advanced biomedical analysis technology that emerged at the end of the 20th century. It integrates sample preparation, biochemical reactions, and result detection into a small microfluidic chip. In the process, the integration, automation, and miniaturization from sample collection to result reporting have been realized. [0003] Sample preparation is the first link of the miniature total analysis system. Due to the wide variety of original samples, such as blood, sputum, saliva, oral test strips, urine, feces, biological tissue cells, pathogenic bacteria and their infected organisms, etc., As a result, the sample preparation is relatively ...

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Problems solved by technology

However, the system is relatively complicated, the volume of the instrument is relatively large, and an external independent centrifuge is still required for sample injection, and due to the heat dissipation of the open fan, the ambient temperature is required to be lower than 30°C and the humidity is not higher than 80%, which is mainly suitable for Use in a lab environment

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