Exposure time determination-based laser speckle blood flow imaging method

Abstract

The invention relates to an exposure time determination-based laser speckle blood flow imaging method, which comprises the following steps of building a laser speckle blood flow imaging system, then, inputting the imaging of a detected object into a computer by a charge coupled device (CCD) camera according to exposure time T, and selecting a target detection point; continuously collecting n frame images from the target detection point by the CCD camera respectively according to the exposure time T1, T2,?-, Tm to obtain m image sets P<T1>(n), P<T2>(n),?- P<Tm>(n), and inputting into the computer for processing; calculating the image gray value contrast K(x, y, Ti) under different exposure time Ti by the computer according to a formula; obtaining a fitted value A<fit> of a reciprocal A of Tao<c> by calculating and fitting a relation between the square of the contrast K2(x, y, Ti) and the exposure time Ti of the CCD camera and the self-correlation function fall time of scattering light-intensity variation Tao<c> according to the laser speckle blood flow imaging theory; and setting the exposure time T0=1.1/A<fit> and performing blood flow detection on the detected object to obtain blood flow velocity distribution. According to the exposure time determination-based laser speckle blood flow imaging method provided by the invention, according to the blood flow velocity of different detected objects, the exposure time of the CCD camera is automatically determined, and the accuracy of the laser speckle blood flow imaging method monitoring blood flow variation is improved.

Description

Technical field [0001] The invention relates to a blood flow imaging test, in particular to a laser speckle blood flow imaging method based on exposure time measurement. Background technique [0002] Blood flow is an important indicator to measure the functional state of the organism. Real-time blood flow monitoring in the body plays an important role in basic research of life sciences, clinical diagnosis and treatment of diseases, and drug development. Therefore, the research of blood flow monitoring methods has always been biomedical The focus of field research. [0003] Laser Speckle Flowgraphy (LSFG) technology is a new type of full-field imaging blood flow fluctuation monitoring technology without scanning. The principle is: when the laser irradiates the biological tissue, the scattered particles in the tissue scatter the incident calculation, and the scattered light produces speckles in the far field through random interference. Due to the movement of some scattered particle...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problem that the current laser speckle blood flow imaging system and method use a fixed exposure time to monitor objects with large differences in blood flow velocity, and the detection error is relatively large, and proposes a laser speckle blood flow imaging system based on exposure time measurement. Flow imaging method to improve the accuracy of laser speckle blood flow imaging method in monitoring blood flow fluctuations

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