Method for detecting seven protein assimilation preparation stimulants in animal body fluid

Abstract

The invention relates to a method for detecting seven protein assimilation preparation stimulants in animal body fluid, which comprises the following steps of: adding an isotope internal standard intermediate solution into the body fluid, extracting by using dichloromethane, separating out a dichloromethane layer, extracting and combining the rest to obtain supernate, transferring the supernate, blowing to be nearly dry, dissolving, carrying out vortex oscillation, and carrying out ultrasonic filtration; measuring the liquid to be measured and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer to obtain spectrogram information; according to the content and the peak area of a to-be-detected component in the mixed standard working solution, the peak area and the concentration of an internal standard substance, the concentration and the peak area of the internal standard substance in the to-be-detected solution, the peak area of the to-be-detected component, the volume of body fluid, the volume of dichloromethane, the volume of the transferred supernatant and the dilution ratio, solving the content of the dehydroepiandrosterone; and calculating the content of the rest of the stimulants by combining the content and the peak area of the to-be-detected component in the mixed standard working solution, the peak area of the to-be-detected component in the to-be-detected solution, the volume of the body fluid, the volume of dichloromethane, the volume of the transferred supernatant and the dilution ratio.

Description

technical field [0001] The invention relates to the technical field of stimulant detection, in particular to a method for detecting 7 kinds of anabolic stimulants in animal body fluids. Background technique [0002] After animals eat stimulant-containing feed, it will cause different degrees of residues in muscles and tissues, and then remain in the human body through the biological chain, which will lead to different degrees of poisoning in the eaters, and athletes are also among them. In order to maintain the fairness and purity of competitive sports and ensure the health of athletes, doping testing has become an indispensable part of all sports competitions. [0003] Seven anabolic stimulants commonly found in animal body fluids, namely dehydroepiandrosterone, α-zearalenol, β-zearalenol, α-zearalenol, β-zearalenol , zearalenone, and zearalenone, they need to be tested. [0004] In order to detect the above-mentioned stimulants, technicians have successively tried method...

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Problems solved by technology

[0004] In order to detect the above-mentioned stimulants, technicians have successively tried methods such as high-performance liquid chromatography (HPLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC / MSD), but these stimulants need to be detected during the detection process. Derivatization will also cause problems such as complicated operation, long detection cycle, and poor sensitivity, which makes it impossible to simultaneously detect 7 kinds of anabolic stimulants in animal body fluids

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