Kit for rapidly detecting content of zilpaterol in food and detection method

Abstract

The invention discloses a kit for rapidly detecting the content of zilpaterol in food and a detection method, and belongs to the technical field of detection, the kit comprises a detection card, an ID chip card and a specification, the detection card is composed of a test strip and a plastic clamping piece, and the test strip comprises 0.5 mg / mL of DNP-BSA and 1 mg / mL of zilpaterol antigen. A quantum dot fluorescence labeling technology and a competitive immunochromatography principle are adopted, a zilpaterol antigen is coated on a certain zone on a nitrocellulose membrane, after a sample is added into a sample adding hole, the sample moves forwards along the membrane due to the capillary action, and when the sample moves to a zone fixed with an antibody, the nitrocellulose membrane is fixed with the zilpaterol antigen. According to the present invention, the corresponding antigen in the sample competes with the antigen, and the quantum dot can make the region not display the fluorescence signal so as to achieve the specific immunodiagnosis, the detection needs to be equipped with the corresponding instrument, the instrument price is low, the size is small, the operation is simple and convenient, the bedside detection can be achieved, the time consumption is about 3-10 min, the sensitivity is high, and the sample consumption is low.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a rapid detection kit and detection method for the content of zilpaterol in food. Background technique [0002] Zipaterol (zilpater01) is a β2-adrenoceptor agonist, a growth promoter that has the same physiological effects as clenbuterol (commonly known as clenbuterol), ractopamine, salbutamol, etc., and is a prohibited drug. However, its chemical structure is different from common B-stimulants, and its toxicity is also 15 times that of ractopamine. However, as the relevant veterinary drug regulatory authorities increase their crackdown on common 8-1 stimulants such as clenbuterol, ractopamine, and salbutamol, and the related detection technologies become more mature, zilpaterol is very likely to be used by criminals and become a food product. A potential killer of security. In view of the above reasons, in 1997, my country introduced albuterol, clenbuterol, ractopamine and o...

AI Technical Summary

Problems solved by technology

But zilpaterol also has serious side effects, which can cause arrhythmia in humans and animals, and can lead to death in severe cases. Like other stimulants, it is listed as a drug that is prohibited from being used in feed and drinking water for livestock.

However, there are still farmers using it illegally in animal husbandry, causing drug residues and causing serious damage to the health of consumers

[0003] At present, there is colloidal gold enzyme-linked immunofluorescence chemiluminescence method for measuring the content of zilpaterol on the market. Colloidal gold enzyme-linked immunosorbent assay needs to be equipped with corresponding instruments, which are low in price, small in size, easy to operate, and can realize large-scale sample detection, which is time-consuming. About 10-20min, the sensitivity is not high, in addition, chemiluminescence needs to be equipped with corresponding instruments, which are expensive and costly