Method for simultaneously detecting 48 stimulants in animal-derived food

Abstract

The invention relates to a method for simultaneously detecting 48 stimulants in animal-derived food, which comprises the following steps of: performing extracting and dewatering according to different types of samples, blow-drying the obtained supernate at a proper temperature, performing vortex oscillation and filtration on the residues obtained by dissolving the reagent to form respective to-be-detected solutions, determining the stimulant in the to-be-detected solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer, comparing the obtained spectrogram information, and judging that a corresponding target object exists in the sample; according to the peak areas of the stimulant and the corresponding internal standard substance, combining the extraction volume, the sample amount and the dilution ratio of each to-be-detected solution in the acquisition process, and determining the contents of zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an external standard method for quantification, and determining the content of all stimulants except for zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an internal standard method for quantification.

Description

technical field [0001] The invention relates to the technical field of stimulant detection in food, in particular to a method for simultaneously detecting 48 kinds of stimulants in animal-derived food. Background technique [0002] In order to maintain the fairness and purity of competitive sports and protect the physical and mental health of athletes, doping testing has become an indispensable part of all sports competitions. There are many types of stimulants with large structural differences, and new stimulants continue to emerge over time. [0003] In order to meet the requirements of doping detection, doping detection needs to continuously improve the detection procedures and improve the sensitivity and accuracy of detection methods. Doping detection technology is also constantly developing. Previously, high-performance liquid chromatography (HPLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC / MSD) and other methods were used for detection. In or...

AI Technical Summary

Problems solved by technology

In order to effectively solve the problems of derivatization, complicated operation, long detection cycle, and poor sensitivity in the detection process of some drugs, more advanced and more sensitive gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry have been adopted. However, it cannot simultaneously determine 48 kinds of stimulants commonly found in animal-derived foods such as pork, beef, chicken, eggs, milk or aquatic products.

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