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A method for determining zilpaterol using cdte quantum dots

A technology of quantum dots and tween-20, which is applied in the field of determination of zilpaterol by using CdTe quantum dots, can solve problems such as inability to accurately and effectively detect the content of zilpaterol

Active Publication Date: 2020-08-04
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the inability to accurately and effectively detect the zilpaterol content in the sample in the prior art, thereby providing a specificity, high sensitivity, and low cost detection agent using CdTe quantum dots. Paltrow's method

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  • A method for determining zilpaterol using cdte quantum dots
  • A method for determining zilpaterol using cdte quantum dots
  • A method for determining zilpaterol using cdte quantum dots

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Embodiment 1

[0071] The present embodiment provides a method for measuring zilpaterol using CdTe quantum dots, comprising the following steps:

[0072] S1. Take 0.2 mL of zilpaterol antibody dilution solution with a concentration of 0.5 mg / mL, add 50 μL of EDC with a concentration of 0.10 mol / L and 50 μL of NHS with a concentration of 0.10 mol / L, leave it at room temperature for 15 minutes, and add 0.10 mL of concentration 1.25×10 -4 The mol / L CdTe quantum dot solution with carboxyl groups on the surface was adjusted to 5.0 mL with pH=7.4 PBS buffer solution (containing 0.4 mol / L NaCl and 0.05% Tween-20), and the reaction was carried out at 4 °C 48h, get CdTe-zilpaterol antibody, spare;

[0073] S2. Using CBS buffer solution with a concentration of 0.05 mol / L and pH=9.6 as the coating medium, dilute the zilpaterol antigen to a concentration of 45 μg / mL, and add it to the wells of the ELISA plate, 100 μL per well, 4°C The reaction was carried out for 10 h, washed with PBS buffer solution ...

Embodiment 2

[0078] The present embodiment provides a method for measuring zilpaterol using CdTe quantum dots, comprising the following steps:

[0079] S1. Take 0.1 mL of zilpaterol antibody with a concentration of 0.3 mg / mL, add 45 μL of EDC with a concentration of 0.08 mol / L and 45 μL of NHS with a concentration of 0.08 mol / L, leave it at room temperature for 12 min, and add 0.2 mL with a concentration of 1 ×10 -4CdTe quantum dots with carboxyl groups on the surface of mol / L were diluted to 5.0 mL with a PBS buffer solution with pH=6.5 (containing 1.0 mol / L NaCl and 0.01% Tween-20), and reacted at 4°C for 46 h, Get CdTe-zilpaterol antibody, spare;

[0080] S2. Use PBS buffer solution with a concentration of 0.01 mol / L and pH=7.4 as the coating medium, dilute the zilpaterol antigen to a concentration of 40 μg / mL, and add it to the wells of the ELISA plate, 100 μL per well, 4°C The reaction was carried out for 5 h, and washed twice (5 min each time) with a PBS buffer solution of pH=6.5 (...

Embodiment 3

[0085] The present embodiment provides a method for measuring zilpaterol using CdTe quantum dots, comprising the following steps:

[0086] S1. Take 0.3 mL of zilpaterol antibody with a concentration of 0.7 mg / mL, add 55 μL of EDC with a concentration of 0.12 mol / L and 55 μL of NHS with a concentration of 0.12 mol / L, leave it at room temperature for 18 minutes, and add 0.1 mL with a concentration of 1.5 ×10 -4 The CdTe quantum dots with carboxyl groups on the surface of mol / L were adjusted to 5.0 mL with PBS buffer solution with pH=7.8 (containing 0.2 mol / L NaCl and 0.1% Tween-20), and reacted at 4°C for 50 h , get CdTe-zilpaterol antibody, spare;

[0087] S2. Using Tris-HCl buffer solution with a concentration of 0.01 mol / L and pH=8.0 as the coating medium, dilute the zilpaterol antigen to a concentration of 55 μg / mL, and add it to the wells of the ELISA plate, 100 μL per well, The reaction was carried out at 4°C for 12 h, and after washing 4 times (2 min each time) with PBS...

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Abstract

The invention discloses a method for measuring zilpaterol by using a CdTe quantum dot. The method comprises the following steps: preparing a CdTe-zilpaterol antibody; preparing a zilpaterol antigen-coated ELISA plate; adding zilpaterol standard solutions and CdTe-zilpaterol antibody solutions with a series of concentration into each hole of the ELISA plate, setting a blank control group simultaneously, incubating and washing; measuring the fluorescence intensity F of each hole in the ELISA plate, setting the fluorescence intensity of the blank control group as F0, calculating the inhibition rate I which is equal to (F-F0) / (Fmax-F0), and drawing a standard curve by taking the inhibition rate as vertical coordinate and the logarithm of the zilpaterol standard solution concentration as horizontal coordinate; and adding a to-be-detected sample and the CdTe-zilpaterol antibody solution into the ELISA plate, incubating, washing, measuring the fluorescence intensity and substituting into thestandard curve to obtain the zilpaterol concentration of the to-be-detected sample. According to the method, the zilpaterol is measured by the CdTe quantum dot, the detection cost is low, the detection time is short, the sensitivity is high and the specificity is high.

Description

technical field [0001] The invention belongs to the field of quantitative detection of zilpaterol, and in particular relates to a method for measuring zilpaterol by utilizing CdTe quantum dots. Background technique [0002] Zilpaterol, a new type of β-receptor agonist, is a growth promoter with the same physiological effects as clenbuterol (commonly known as clenbuterol), ractopamine, salbutamol, etc. It is a prohibited drug, but Its chemical structure is different from common β-receptor agonists, and its toxicity is also 15 times that of ractopamine, which can transfer nutrients in animals from fat to muscles, showing a nutrient redistribution effect, and then regulating the substances in the animal body. Metabolism, enhance fat decomposition, promote protein synthesis, and significantly enhance leptin in ketone bodies, which can cause muscle tremors, heart failure, and even death in humans or animals, causing harm to people's health and life safety. [0003] Currently, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 魏益华张金艳罗林广李瑞丽赖艳
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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