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A method for determination of growth promoter residues of zilpaterol, sibuterol, clenpro and bambuterol in beef

A growth-promoting agent and a method for determination are applied in the field of determination of zilpaterol, cibuterol, clembuterol and bambuterol growth-promoting agent residues in beef, and can solve problems such as affecting human health.

Inactive Publication Date: 2017-04-05
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, if the beef containing these drugs is eaten by humans, side effects such as heart palpitations, limb muscle tremors, and arrhythmia will occur, which will seriously affect human health.
At present, there is no relevant determination method for the above four drugs remaining in beef

Method used

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  • A method for determination of growth promoter residues of zilpaterol, sibuterol, clenpro and bambuterol in beef
  • A method for determination of growth promoter residues of zilpaterol, sibuterol, clenpro and bambuterol in beef
  • A method for determination of growth promoter residues of zilpaterol, sibuterol, clenpro and bambuterol in beef

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Embodiment Construction

[0014] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0015] Reagents and Instruments

[0016] Growth-promoting agents: Zipaterol, sibuterol, crenpro, bambuterol standard products, all with a purity greater than 98.0%. Formic acid (HCOOH): analytically pure. Ethyl acetate (C 4 H8O 2 ): analytically pure. Methanol (CH 3 OH): chromatographically pure. Perchloric acid (HClO 4 ): Premium pure. Sodium Hydroxide (NaOH): Superior grade. Ammonia (NH 4 OH): Superior grade. Ammonium acetate (CH 3 COONH 4 ). 0.1 mol / L perchloric acid solution. 5 mol / L sodium hydroxide solution. 5% vol ammonia methanol solution (ammonia methanol). 0.1% vol formic acid in water. β-glucuronidase / arylsulfatase (β-glucuronidasc / arylsulfatasc): enzyme activity ≥ 100,000 units / min.

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Abstract

The invention discloses a method for measuring residual quantities of growth promoting agents such as zilpaterol, cimbuterol, clenproperol and bambuterol in beef. The method includes hydrolyzing beef samples by the aid of beta-glucuronidase / aryl sulfatase; centrifuging the beef samples under the condition of pH (potential of hydrogen) of 1.0+ / -0.2; regulating pH of supernatant until the pH reaches 9.5+ / -0.2; sequentially extracting the supernatant by the aid of ethyl acetate and tert-butyl methyl ether; combining organic phases with one another; drying the organic phases by means of blowing; dissolving the organic phases by the aid of formic acid; enabling the organic phases to pass cation exchange solid-phase extraction columns; sequentially leaching the organic phases by the aid of 2% formic acid, water and methanol; eluting the organic phases by the aid of ammonium methanol; drying eluent by means of blowing; dissolving the organic phases by the aid of formic acid and methanol aqueous solution; filtering the organic phases by the aid of films; feeding filter liquid in liquid chromatograph / mass spectrometers to obtain the residual quantities of the growth promoting agents in the beef according to standard curves. The method has the advantages that the four growth promoting agents including the zilpaterol, the cimbuterol, the clenproperol and the bambuterol can be quickly detected by the aid of the method, and residues of the four growth promoting agents which are four types of medicine in the beef can be monitored.

Description

technical field [0001] The invention relates to a method for determining the residual amount of growth-promoting agents such as zilpaterol, sibuterol, clenpro, bambuterol and the like in beef. Background technique [0002] Drugs such as zilpaterol, sibuterol, clenpro, and bambuterol have obvious effects on animals such as promoting growth, increasing lean meat percentage, and improving production performance. However, if the beef with these drugs remaining is eaten by people, side effects such as heart palpitations, limb muscle tremors, and arrhythmia will occur, seriously affecting human health. At present, there is no relevant determination method for the above four drugs remaining in beef. In order to ensure the food safety of the majority of residents, eliminate and monitor the residues of the above four drugs in beef, and prevent passiveness in emergencies, it is necessary to detect the content of the above four growth-promoting drugs in beef tissue. Contents of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N1/34
Inventor 熊琳李维红李润林杨晓林高雅琴
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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