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A method for determining zilpaterol using cdte quantum dots

A technology of quantum dots and concentration, which is applied in the field of determination of zilpaterol by using CdTe quantum dots, which can solve the problems of inability to accurately and effectively detect the content of zilpaterol

Active Publication Date: 2020-08-04
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the inability to accurately and effectively detect the zilpaterol content in the sample in the prior art, thereby providing a specificity, high sensitivity, and low cost detection agent using CdTe quantum dots. Paltrow's method

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  • A method for determining zilpaterol using cdte quantum dots
  • A method for determining zilpaterol using cdte quantum dots
  • A method for determining zilpaterol using cdte quantum dots

Examples

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Embodiment 1

[0070] The present embodiment provides a method for utilizing CdTe quantum dots to measure zilpaterol, comprising the following steps:

[0071] S1. Take 1.0 mL of zilpaterol antigen solution with a concentration of 1 μg / mL, add 50 μL of EDC with a concentration of 0.10 mol / L and 50 μL of NHS with a concentration of 0.10 mol / L, place it at room temperature for 15 minutes at 25°C, and add 1.0 mL of EDC with a concentration of 0.10 mol / L. 1.25×10 - 4 The surface of mol / L has the CdTe quantum dot solution of carboxyl groups, with the PBS buffer solution (containing 0.5mol / L of NaCl and mass concentration is the Tween-20 that mass concentration is 0.1%) with pH=7.4 to be settled to 5.0mL, 4 React at ℃ for 48 hours to obtain the CdTe-zipaterol antigen for later use;

[0072] S2. Use CBS buffer solution with a concentration of 0.05 mol / L and pH=9.6 as the coating medium, dilute the zilpaterol antibody to a concentration of 30 μg / mL, add to the wells of the microplate, 100 μL per we...

Embodiment 2

[0077] The present embodiment provides a method for utilizing CdTe quantum dots to measure zilpaterol, comprising the following steps:

[0078] S1. Take 1.5 mL of zilpaterol antigen with a concentration of 0.5 μg / mL, add 45 μL of EDC with a concentration of 0.08 mol / L and 45 μL of NHS with a concentration of 0.08 mol / L, place it at room temperature for 12 minutes at 26°C, and add 1.0 mL of EDC with a concentration of 0.08 mol / L. 1×10 -4 mol / L CdTe quantum dots with carboxyl groups on the surface, dilute to 5.0 mL with PBS buffer solution (containing 1.0 mol / L NaCl and 0.02% Tween-20 with a mass concentration of 0.02%) at pH = 6.5, and react at 4°C for 46 h , to get CdTe-zipaterol antigen, spare;

[0079]S2. Use PBS buffer solution with a concentration of 0.01mol / L and pH=7.4 as the coating medium, dilute the zilpaterol antibody to a concentration of 60 μg / mL, add it to the wells of the microtiter plate, 100 μL per well, and keep at 4°C. After reacting for 5 h, wash twice (5 ...

Embodiment 3

[0084] The present embodiment provides a method for utilizing CdTe quantum dots to measure zilpaterol, comprising the following steps:

[0085] S1. Take 0.5 mL of zilpaterol antigen with a concentration of 2 μg / mL, add 55 μL of EDC with a concentration of 0.12 mol / L and 55 μL of NHS with a concentration of 0.12 mol / L, place it at room temperature for 18 minutes at 24°C, and add 1.5 mL of EDC with a concentration of 0.12 mol / L. 1.5×10 -4 mol / L of CdTe quantum dots with carboxyl groups on the surface, dilute to 5.0mL with pH=7.8 PBS buffer solution (containing 0.1mol / L NaCl and 0.5% Tween-20), and react at 4°C for 50h , to get CdTe-zipaterol antigen, spare;

[0086] S2. Use Tris-HCl buffer solution with a concentration of 0.01mol / L and pH=8.0 as the coating medium, dilute the zilpaterol antibody to a concentration of 15 μg / mL, add it to the wells of the microtiter plate, 100 μL per well, React at 4°C for 12 h, wash 4 times (2 min each time) with PBS buffer solution (containing...

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Abstract

The invention discloses a method for utilizing CdTe quantum dots to measure zZilpaterol. The method comprises the steps that a CdTe-zZilpaterol antigen is prepared; an enzyme-labeledELISA plate coatedwith a zZilpaterol antibody is prepared; a Zzilpaterol standard solution and a CdTe-zZilpaterol antigen solution in the series concentrations are added into holes of the enzyme-labeledELISA plate, meanwhile, a blank control group is set, and incubation and washing are conducted; the fluorescence intensity F of each hole in the enzyme-labeledELISA plate and the fluorescence intensity fF0 of the blank control group are measured, the inhibition ratio I is calculated according to the formula that I=(F-F0) / (Fmax-F0), the inhibition ratio serves is treated as the vertical coordinate, the logarithmof the concentration of the zZilpaterol standard solution serves as the horizontal coordinateabscissa, and a standard curve is drawn; and a to-be-detected sample and the CdTe-zZilpaterol antigen solution are added into the enzyme-labeledELISA plate to be subjected to incubation and washing, the fluorescence intensity is measured, the standard curve is substituted in, and the concentration of zZilpaterol in the to-be-detected sample is obtained. Zilpaterol is measured by utilizing the CdTe quantum dots, the detection cost is low, the detection time is short, the sensitivity is high, and the specificity is good.

Description

technical field [0001] The invention belongs to the field of quantitative detection of zilpaterol, in particular to a method for measuring zilpaterol by using CdTe quantum dots. Background technique [0002] Zipaterol, a new type of β-receptor agonist, is a growth promoter that has the same physiological effects as clenbuterol (commonly known as clenbuterol), ractopamine, albuterol, etc. It is a prohibited drug, but Its chemical structure is different from common β-receptor agonists, and its toxicity is 15 times that of ractopamine. It can transfer the nutrients in the animal body from fat to muscle, showing a nutrient redistribution effect, and then regulate the substances in the animal body. Metabolism, enhance fat decomposition, promote protein synthesis, and significantly enhance leptin in ketone bodies, which can cause human or animal muscle tremors, heart failure, and even death, causing harm to the health and life safety of the people. [0003] Currently the main det...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N33/535
CPCG01N21/6428G01N33/535
Inventor 张金艳魏益华罗林广邱素艳张大文
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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