A rapid detection method for zilpaterol residues in pork

A technology for quantitative detection of pork, applied in measurement devices, preparation of samples for testing, material excitation analysis, etc. The effect of the convenient detection method

Active Publication Date: 2021-12-28
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatography and mass spectrometry generally have defects such as long pretreatment process, complicated and technical operation, and expensive instruments and equipment, which make it difficult to meet the requirements for rapid screening of a large number of samples on site

Method used

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  • A rapid detection method for zilpaterol residues in pork
  • A rapid detection method for zilpaterol residues in pork
  • A rapid detection method for zilpaterol residues in pork

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A method for quantitatively detecting zilpaterol in pork, comprising the steps of:

[0068] (1) Preparation of Raman enhanced substrate:

[0069] Soak the used glassware (round bottom flask) and rotor with aqua regia for 12 hours, take it out and wash it with water until it is odorless, then rinse it with ultrapure water three times, dry it in an oven at 60°C, and set it aside; the prepared mass fraction is 1% trisodium citrate Aqueous solution, 10mg / mL potassium chloroaurate solution, set aside; raise the temperature of the oil bath to 120°C and keep it constant; set the magnetic stirrer parameters: 565r / min;

[0070] Add 47mL of ultrapure water and 3mL of potassium chloroaurate into the round bottom flask and mix thoroughly; put the round bottom flask into a 120°C oil bath while stirring with a magnetic stirrer and keep the temperature constant until the solution boils; add 2mL of 1 % Trisodium citrate aqueous solution continued to stir at 120°C for 20 minutes; coole...

Embodiment 2

[0087] The optimization of embodiment 2 extraction solvent

[0088] Adjust the extraction solvent ethyl acetate in the pork extract in Example 1 to be acetonitrile or isopropanol, and keep the others consistent with Example 1 for detection.

[0089] The test results are: when it is found that the extraction solvent is acetonitrile or isopropanol, the characteristic peaks of zilpaterol do not appear, and the extraction effect of ethyl acetate is better.

Embodiment 3

[0090] The optimization of embodiment 3 detection ratio

[0091] Adjust the volume ratio of the standard curve simulation solution and the gold sol in Example 1, the volume ratio of the zilpaterol pork standard extract and the gold sol to 1:1 or 3:1, and keep the others consistent with Example 1 for detection .

[0092] The test result is: selected 1113cm -1 The Raman peak at is used as a reference, and the gold sol is mixed with the test solution according to different proportions, and the results are as follows Figure 6 As shown, the signal intensity is the highest when the volume of gold sol and the solution to be tested is 1:2.

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Abstract

The invention discloses a rapid detection method for zilpaterol residues in pork, belonging to the field of food detection. The present invention uses density functional theory and the standard solution of zilpaterol to determine the Raman characteristic peak of zilpaterol; the Raman characteristic peak signal is strong and the characteristic peak of peak shape is 1113cm ‑1 Establish quantitative analysis curve Y=5214.7X‑35.844, correlation coefficient R 2 It is 0.9355; Afterwards, the pork sample is pretreated and extracted with an extraction solvent to obtain a test solution; then, according to the Raman spectrum and the standard curve of the test solution, the concentration of zilpaterol in the test sample is obtained by testing. The minimum detectable concentration of zilpaterol in pork detected by the method of the present invention is 5 mg / L, qualitative and quantitative analysis can be performed on the residue of zilpaterol in pork, and the detection time of a single sample is controlled within 3 minutes.

Description

technical field [0001] The invention relates to a rapid detection method for zilpaterol residues in pork, belonging to the field of food detection. Background technique [0002] Veterinary drug residues refer to the parent compound and / or its metabolites of veterinary drugs contained in any edible part of animal products, as well as impurities related to veterinary drugs. [0003] Veterinary drugs and feed additives remaining in animal-derived food enter the human body along the food chain, posing a potential threat to human health. This threat has attracted more and more attention. With the change of people's demand for animal-derived food, the international requirements for animal residues in animal-derived food are getting higher and higher, and veterinary drug residues in animal-derived food have gradually become a focus of attention all over the world. Zipaterol is a β-receptor agonist, commonly known as "clenbuterol". The application of food animals originated in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N1/28
CPCG01N21/658G01N1/28
Inventor 谢云飞董祥辉施思倩姚卫蓉孙莹莹邱险辉
Owner JIANGNAN UNIV
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