Method for detecting 35 veterinary drug residues in meat product at same time

Abstract

The invention provides a method for detecting 35 veterinary drug residues in a meat product at the same time. The method can be used for detecting veterinary drug residue condition of the meat productsuch as pork, different national standard methods only can detect the same class of veterinary drugs, and the method provided by the invention can detect 35 different classes of veterinary drugs at the same time. Determination shows that the linearity of the 35 veterinary drugs in 20-200ng / mL is good, and correlation coefficient is higher than 0.99; low-concentration recovery rate of a pork sample at 10 microg / kg is 70-120%, and relative standard deviation is less than 0.2; and detection limit is less than or equal to 10 microg / kg, and the convenient examination requirement is met. The methodprovided by the invention is good in selectivity, high in sensitivity and simple and easy to operate, and a reagent is saved.

Description

Technical field [0001] The invention belongs to the field of analytical chemistry, and specifically relates to a method for simultaneously detecting 35 veterinary drug residues in meat products by using liquid chromatography-tandem quadrupole mass spectrometry. Background technique [0002] Veterinary drug residues refer to the residues of prototype drugs or their metabolites, including impurities related to veterinary drugs, that are accumulated or retained in livestock and poultry bodies or products (such as eggs, milk, meat, etc.) after drug use. The use of pesticides has brought many negative effects while increasing crop yields. [0003] Meat products refer to cooked meat products or semi-finished products made by seasoning with livestock and poultry meat as the main raw material, such as sauced stewed meat, barbecued meat, etc. That is to say, all meat products that use livestock and poultry meat as the main raw material and add seasonings are called meat products, including...

AI Technical Summary

Problems solved by technology

At present, most of the pretreatment methods for veterinary drug residues in meat products use traditional extraction and purification methods, which consume a large amount of solvent, cumbersome operation process, long analysis cycle, and easy to cause environmental pollution

Compared with other crops, the specificity and complexity of meat products have stricter pretreatment requirements, and more advanced pretreatment technologies in other fields cannot be directly used, which undoubtedly increases the workload and cost of experiments. , runs counter to the development trend of veterinary drug residue analysis

So far, in the field of detection of veterinary drug residues in meat products, liquid chromatography-mass spectrometry has been widely used in the analysis of veterinary drug residues. The research scope mainly includes the analysis of single or single type of veterinary drug residues, and the simultaneous analysis of multiple types of veterinary drug residues. less

Therefore, there are problems such as long analysis time, inability to analyze and detect multiple types of veterinary drug residues at the same time, cumbersome analysis process, and high cost.

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