Electrochemical immunosensor used for CLB detection, and preparation method thereof

Abstract

The invention provides an electrochemical immunosensor used for CLB detection, and a preparation method thereof. The electrochemical immunosensor possesses a working electrode based on a MoS2-AuPt nanometer composite and a biotin-streptavidin system. The electrochemical immunosensor is high in sensitivity (detection limit is 6.86pg.ml<-1>), the specificity is high, the repeatability is acceptable,and the electrochemical immunosensor can be used for detection of practical pork samples.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an electrochemical immunosensor for CLB detection and a preparation method. Background technique [0002] Clenbuterol (CLB) is a sympathomimetic amine of the beta-agonist family used as a broncholytic drug in asthma, especially in the setting of chronic disease. When used as a food additive in food producing animals, CLB can also promote muscle growth and reduce fat content. Therefore, CLB is called "Clenbuterol". For economic benefit, many producers illegally add CLB to livestock feed to increase lean meat production. However, CLB has a long half-life and is slowly metabolized in livestock. If people eat meat containing CLB for a long time, especially children and the elderly, corresponding clinical symptoms and signs will appear, such as muscle tremor, glaucoma, mental anxiety, palpitations, and even death. For the sake of public health and food safety, many countries have enac...

AI Technical Summary

Problems solved by technology

[0003] In view of the shortcomings of the prior art described above, the object of the present invention is to provide an electrochemical immunosensor for CLB detection and a preparation method for solving the problem that the detection limit of clenbuterol CLB in the prior art is not low enough, Long response time, high cost, poor sensitivity, etc.

Images