Chain reaction method for detecting polymerase of salmonella choleraesuls

A Salmonella, chain reaction technology, applied in biochemical equipment and methods, microbial determination/inspection, resistance to vector-borne diseases, etc., can solve problems such as failure to directly detect Salmonella choleraesuis

Inactive Publication Date: 2007-02-14
曾浩洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, there is no rapid and direct method for the detection of Salmonella choleraesuis

Method used

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  • Chain reaction method for detecting polymerase of salmonella choleraesuls
  • Chain reaction method for detecting polymerase of salmonella choleraesuls
  • Chain reaction method for detecting polymerase of salmonella choleraesuls

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Embodiment

[0030] 1. Source of the strain:

[0031] The present invention uses Salmonella isolates of different serotypes and strains of other genera, including Enterobacteriaceae, such as Escherichia coli, Citrobacter, Shigella and Klebsiella (Klebsiella) genus (as shown in Table 1 and Table 2). These strains were obtained from the American Type Culture Collection (ATCC), U.S.A.; the United States Department of Agriculture (USDA), U.S.A.; the Bioresource Collection and Research Center, Taiwan. (BCRC), Taiwan); National Ping-Tong University of Technologies, Taiwan; Center for Disease Control (CDC), U.S.A.; New York City Department of Health and Mental Hygiene (New York CityDepartment of Health and Mental Hygiene (NYC DOMHM), U.S.A.); Bureau of Food and Drug Administration, Department of Health (BFDA), Taiwan; Food Research Institute (FRI), University of Wisconsin of Wisconsin, U.S.A.) and Taiwan Center for Disease Control (CDC), Taiwan). The isolated strain of Salmonella choleraesuis ...

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Abstract

The invention discloses a hog cholera salmonella detection method. It also discloses its PCR primer and kit. The PCR detection method can be used in human whole blood and other clinic sample, animal and human excreta sample, pork sample etc which can rapidly gain the detecting result and has high sensitivity.

Description

technical field [0001] The invention relates to a method for detecting Salmonella choleraesuis, a pair of PCR primers and a kit. In particular, the present invention relates to a method for detecting Salmonella choleraesuis using the flagellar gene fliC, a pair of PCR primers and a kit. Background technique [0002] Salmonella enterica serovar Choleraesuis (Salmonella choleraesuis) is one of the most frequently found serotypes in pigs, and the host of this serotype strain is humans and pigs (Bümler et al., Infect.Immun., 66: 4579- 4587). Salmonella choleraesuis can cause salmonellosis in pigs with symptoms of sepsis and enteritis. The literature indicates that a small number of pigs infected with Salmonella can spread rapidly and subsequently infect many more pigs before slaughter (Gary et al., Appl. Environ. Microbiol., 62: 141-146). In Taiwan, isolates of Salmonella choleraesuis were highly multi-drug resistant and became widespread pathogens, posing a major problem in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCY02A50/30
Inventor 曾浩洋邱采新
Owner 曾浩洋
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