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Method for quickly detecting delicious substance inosinic acid in raw and fresh pork based on Raman spectrum

A technology of Raman spectroscopy and inosinic acid, which is applied in the field of quality and safety detection of livestock meat products, can solve the problems of difficult evaluation of pork flavor quality and difficult rapid and large-scale detection of inosinic acid, and achieve the effect of improving detection speed and efficiency

Inactive Publication Date: 2015-10-07
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of difficult evaluation of pork flavor quality, and the difficulty of rapid and large-scale detection of inosinic acid content in the detection process based on traditional chemical detection methods. Rapid detection method for nucleotides

Method used

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  • Method for quickly detecting delicious substance inosinic acid in raw and fresh pork based on Raman spectrum
  • Method for quickly detecting delicious substance inosinic acid in raw and fresh pork based on Raman spectrum
  • Method for quickly detecting delicious substance inosinic acid in raw and fresh pork based on Raman spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Example 1: Detection of the content of inosinic acid in fresh pork on the day of commercial sale

[0045] A. Determination of Raman Spectrum of Inosinic Acid Standard

[0046] The Raman spectrogram of standard inosinic acid was detected by the InVia micro confocal Raman spectrometer of Renishaw Company. The laser wavelength was 633nm, the laser power was 17mW, the exposure time was 5s, and the average value of scanning times was 3 times, and the standard inosinic acid was collected. Man spectrum, Raman spectrum range 1000-4000cm -1 ;

[0047] B. Obtain the Raman spectrum of fresh pork samples sold in the market on the same day

[0048] Take the longest muscle of the pork back, use a slicer to cut a 1mm thin and uniform pork slice sample according to the pork texture, place the sample on a glass slide and place it on the stage of the micro-Raman spectrometer, and select the automatic focus adjustment to adjust the micro-Raman laser Probe, so that the sample is loca...

Embodiment 2

[0061] 2. Example 2: Detection of inosinic acid content in pork placed at different times after live pigs were slaughtered

[0062] a. Determination of Raman Spectrum of Inosinic Acid Standard

[0063] The Raman spectrogram of standard inosinic acid was detected by the InVia micro confocal Raman spectrometer of Renishaw Company. The laser wavelength was 633nm, the laser power was 17mW, the exposure time was 5s, and the average value of scanning times was 3 times, and the standard inosinic acid was collected. Man spectrum, Raman spectrum frequency range 1000-4000cm -1 ;

[0064] b. Obtain the Raman spectrum of the pork sample

[0065] Live pigs were slaughtered and cleaned at room temperature (20±1°C), and the IMP content was measured at 0, 1, 2, 3, 4, 6, 12, and 24 hours respectively, and a slicer was used to cut out 1 mm thick and uniform meat according to the pork texture. For the pork slice sample, place the sample on a glass slide and place it on the stage of the micro-...

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Abstract

The invention discloses a method for quickly detecting a delicious substance inosinic acid in raw and fresh pork based on a Raman spectrum, and relates to the technical field of livestock meat quality and safety detection. The method comprises the following steps: 1, measuring the Raman spectrum of an inosinic acid standard product; 2, obtaining the Raman spectrum of a raw and fresh pork sample; 3, measuring the content of inosinic acid by a chemical method; 4, performing Raman spectrum correction; 5, preferably selecting an inosinic acid feature peak; 6, calculating a feature Raman peak area; 7, constructing a detection model for the content of inosinic acid. According to the method, the delicious substance inosinic acid in the raw and fresh pork can be quickly detected by a microscopic confocal Raman spectrum technology, so that an effective quick pork delicious substance inosinic acid detection method which is easy to operate, low in cost and short in time is searched; the detection method is simple, safe and efficient and can meet the industrial production demand and the requirement of a customer on the quality of a product; therefore, the evaluation requirement on the flavor quality of a pork product in an actual production process is met.

Description

technical field [0001] The invention relates to the technical field of quality and safety detection of livestock meat products, in particular to a rapid detection method for the umami substance inosinic acid in fresh pork based on Raman spectroscopy. Background technique [0002] Pork is delicious, nutritious and easily digested and absorbed by the human body, and has always been loved by people. 1. my country is the largest pork production country in the world. Since 1972, pork production has always ranked first in the world. Pork and its meat products are also the main meat food of Chinese residents and an important source of income for Chinese farmers, and occupy a very important position in the national economy; they have played an important role in improving people's lives, increasing farmers' income, and ensuring social stability. , Pork products have been widely concerned by all walks of life. With the continuous improvement of people's living standards, the consump...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N30/00
Inventor 介邓飞魏萱
Owner HUAZHONG AGRI UNIV
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