64 results about "Confocal raman spectroscopy" patented technology

The invention belongs to the micro-spectrum imaging technical field and relates to a differential confocal raman spectral test method. The method integrates the technical characteristics of the differential confocal detection method and the raman spectral detection method, forms a test method capable of realizing sample microarea spectral detection, precisely catches focus positions of excitation light beams through the differential confocal technology, detects raman spectra of corresponding positions, simultaneously adopts a designed pupil filter, sharpens Airy disc major lobes of a differential confocal raman spectral system, improves the microarea raman spectral detectability and precisely acquires microarea space spectrum information which comprises spectral information and position information of microarea samples. The method obviously improves the microarea spectral detectability of a confocal raman spectromicroscope, has absolute tracking zero points and bipolar tracking characteristics, realizes absolute measurement of physical dimension, can be widely applied in the technical fields such as biomedicine, life sciences, biophysics, biochemistry, industrial precision detection and so on to perform high-precision detection of geometric positions and spectral characteristics of microareas, and has very important application prospect.

The invention belongs to the technical field of optical microscopic imaging and spectral measurement, and relates to a method and a device for confocal Raman spectrum detection with high spatial discrimination. According to the method and the device, confocal technique is integrated into the spectrum detection; nondestructive separation is carried out on Rayleigh scattering light and Raman scattering light by using of a dichroic beam splitting system (13); by using the characteristic that the maximum value of a confocal curve (34) corresponds to a focal position accurately, spectral information exciting a light spot focal position is accurately acquired through maximizing; and the spectrum detection with the high spatial discrimination is achieved, and the method and the device which are capable of achieving the sample microcell spectrum detection with the high spatial discrimination are formed. The method and the device for the confocal Raman spectrum detection with the high spatial discrimination have the advantages of being accurate in positioning, high in spatial discrimination, high in spectrum detection sensitivity and the like, and has broad application prospect in the fields of biomedicine, court evidence collection and the like.

The invention relates to a spectral pupil laser confocal Brillouin-Raman spectrum measuring method and device and belongs to the technical field of microscopy spectral imaging. The device comprises a light source system for generating stimulating light beams, a measuring objective lens, a lighting pupil, a collecting pupil, a dichroic light splitting device, a spectroscope, an Raman spectrum detecting device, a Brillouin spectrum detecting device, a spectral pupil laser confocal detecting device, a three-dimensional scanning device, a displacement sensor and a data processing unit. The method and the device provided by the invention has the advantages that the abandoned rayleigh scattering light in confocal Raman spectrum detection is utilized to build a spectral pupil confocal microimaging system to realize the high-resolution imaging of the three-dimensional geometric position of a sample, the basic property and multiple cross effects of a substance are obtained by detecting the abandoned brillouin scattering light in confocal Raman spectrum detection and further the stress, elastic parameters and density of a material can be measured; the advantages of the confocal Raman spectrum detection technology and the confocal Brillouin spectrum detection technology are utilized to complement each other, and the comprehensive measurement and decoupling of multiple property parameters of the material are realized.

In one aspect, the present invention relates to a probe using integrated confocal reflectance imaging, confocal Raman spectroscopy, and gross spatial imaging for non-invasively evaluating a target of interest of a living subject. In one embodiment, the probe includes a casing with a first end and an opposite, second end, a first optical port, a second optical port, and a third optical port, where the first and second optical ports are located at the first end of the casing and the third optical port is located at the second end of the casing such that the first and third optical ports define a first optical path between them and the second and third optical ports define a second optical path between them, respectively, where each of the first and second optical paths has a first portion and a second portion, where the second portions of the first and second optical paths are substantially overlapped and proximal to the third optical port, and where the probe also includes a collimation lens, a coupling lens, an objective lens assembly, a first mirror, a second mirror, a third mirror, a band pass filter, a long pass filter, a scanning member, an electronic imaging device, and a focus control device, where the collimation lens, the band pass filter, and the second mirror are placed at the first portion of the first optical path, where the coupling lens, the long pass filter, and the first mirror are placed at the first portion of the second optical path, and where the third mirrors, the scanning member, and the objective assembly are placed at the overlapped second portion of the first and second optical paths.

The invention belongs to the technical field of microscopic imaging and spectral measurement and relates to a high resolution spectral imaging and detection method and apparatus which combine confocal microscopic technology and spectral detection technology together, realize integration of images and spectrums and are used for three dimensional morphology reconstruction and micro-area morphological performance parameter measurement of a variety of samples. The method and apparatus utilize Rayleigh light abandoned by a traditional confocal Raman system and confocal technology for detection of the position of a sample, employs a spectral detection system for spectral detection and uses Brillouin diffusion light abandoned by a traditional confocal Raman detection technology to test properties like elasticity and piezoelectricity of a material, thereby realizing measurement of micro-area high spatial resolution morphological parameters of a sample. The method and apparatus provided by the invention have the advantages of accurate positioning, high spatial resolution, high spectral detection sensitivity, controllable measured spot size, etc. and have wide application prospects in fields like biomedicine, evidence collection of court, micro and nano-fabrication, material engineering, engineering physics, precise metering and physical chemistry.

The invention belongs to the field of a microimaging and spectral measurement technology and relates to a laser differential confocal Brillouin-Raman spectroscopy measuring method and a device thereof which can be used in micro-area morphological parameter comprehensive test and high-resolution imaging of a sample. According to the method and the device, a differential confocal technology is incorporated into spectrum detection. Sample position detection is performed by the differential confocal technology; spectrum detection is conducted by a spectrum detection system; and properties, such as elasticity, piezoelectricity and the like, of a material are tested by the use of brillouin scattering light abandoned by a traditional confocal Raman spectrum detection technology. Thus, micro-area high-spatial resolution morphological parameter measurement of a sample is realized. The method and the device have advantages of accurate positioning, high spatial resolution, high spectrum detection sensitivity, controllable measured focusing spot size and the like, and have a wide application prospect in fields of biomedicine, evidence obtaining in court, micro and nano-fabrication, materials engineering, engineering physics, precision metrology, physical chemistry and the like.

The invention discloses a microscopic confocal Raman spectrometer, comprising a microscopic confocal Raman module and a single-grating spectrometer which is matched with the microscopic confocal Raman module, wherein the microscopic confocal Raman module comprises a reflecting mirror, an angle-adjustable optical filter bracket, a sample monitoring optical path, a Raman optical filter, a microscopic objective lens, a collecting lens and a size-adjustable pinhole. Laser is focused to a sample by using the microscopic confocal Raman module, so that the sample and the corresponding laser spots are observed microscopically, and a Raman signal is microscopically confocal to the size-adjustable pinhole simultaneously, therefore the Raman signal is detected by using the single-grating spectrometer. The invention provides a novel microscopic confocal Raman spectrometer with the characteristics of simpleness, economy and convenience for adjustment, so that the microscopic observation of the sample and the corresponding laser spots can be achieved, besides, the microscopic confocal Raman spectrometer plays a potential role on Raman spectrum measurement of materials.

Use of Confocal Raman Spectroscopy (CRS) for dermatological studies, including a method for determining the thickness of the Stratum Corneum (SC) on a test area of the skin, and to a method for quantifying the effectiveness of a skin care composition. The methods of the invention can be carried in vitro (either artificial skin or a sample of skin) or in vivo (directly on the human skin of a person).

The invention discloses a crude oil identification method based on microscopic confocal Raman spectrum. The method comprises the following steps: by changing parameters of a Raman spectrometer, respectively acquiring three different types of fingerprint data of a crude oil sample: fingerprint data with significant Raman characteristics and weak fluorescence characteristics, fingerprint data with significant fluorescence characteristics and weak Raman characteristics, and fingerprint data with significant Raman characteristics and fluorescence characteristics; and carrying out clustering analysis on the fingerprint data so as to obtain crude oil clustering results. According to the invention, uncertain factors and errors caused by deducting a baseline by traditional Raman data processing software in a polynomial fitting mode are avoided; due to the mutual complementation and verification of the clustering results of the three types of fingerprint data, the precision and credibility of crude oil identification results can be significantly improved, an important basis can be provided for the responsibility identification and economic compensation of oil spilling events, and a theoretical basis can be provided for exploring a migration and transformation mechanism of oil spilling in pollution abatement and environmental modification processes.

The invention relates to a femtosecond laser processing parameter confocal Raman microspectroscopy in-situ monitoring method and device, and belongs to the technical field of laser precision detectionand femtosecond laser processing and manufacturing. A laser confocal axial monitoring module with a high axial resolution is organically integrated with a femtosecond laser processing system, a confocal system curve maximal point is utilized for conducting nanoscale monitoring and sample axial processing size measurement on the sample axial position, real-time focus fixing of the sample axial position and high-precision measurement of the size of a processed micro-nano structure are achieved, and the drifting problem and the high-precision online detection problem in the measurement process are solved; a confocal Raman microspectroscopy detection module is utilized for monitoring and analyzing information such as molecular structures of sample materials subjected to femtosecond laser processing, the information is integrated through a computer, microstructure femtosecond laser high-precision processing and micro-domain form performance in-situ monitoring analysis are integrated, and the controllability of microstructure femtosecond laser high-precision processing and the processing quality of the samples are improved.

The invention relates to an animal active single-cell sample treatment method wherein the animal active single-cell sample is for surface-enhanced raman spectroscopy detection. The treatment method comprises: dissolving solid silver nitrate in deionisation water and heating water to 100 DEG C., dripping sodium citrate solution into water and stirring the water until boiling, centrifuging the boiled water and taking the concentrated silver sol at the lower layer; preparing the single-cell suspension by culturing the animal active single-cell sample to be detected in the cell culture medium; performing the second centrifugation of the prepared silver sol and removing away the supernatant and adding the cell culture medium to prepare silver sol suspension and mixing the silver sol suspension with the single-cell suspension according the volume ratio of 1:4, transfering the mixture into an electric-shock cup sample cell and performing electroporation after ice bathing, and then rinsing the mixture out from the sample cell and culturing the mixture in an incubator after ice bath, finally the sample can be detected using a confocal Raman spectrometer. The treatment method has features of simpleness, quick speed, high reliability, good generality and clear and stable surface-enhanced raman spectroscopy of the inside of active cell.

The invention relates to a laser dual-axis confocal Brillouin-Raman spectral measurement method and apparatus realizing integration of images and spectrums, belonging to the technical field of microscopic spectral imaging. According to the invention, dual-axis confocal microscopy and spectral detection technology are organically fused, Rayleigh light is used for assisted detection, Raman spectral detection technology and Brillouin spectral detection technology are cooperatively used to realize high spatial discrimination image-spectrum integrated detection of a system, and high spatial resolution is achieved. The apparatus has three modes, i.e., a three-dimensional chromatographic geometric imaging mode, a spectral detection mode and a micro-area image-spectrum chromatographic imaging mode, obtains basic properties and a plurality of crossing effects of substances by detecting abandoned Brillouin diffusion light in confocal Raman spectral detection, realizes acquisition of a plurality of parameters like the micro-area morphology, state, texture and attributes of a measured sample in virtue of the characteristic that advantages of confocal Raman spectral detection technology and confocal Brillouin spectral detection technology are complementary, and has wide application prospects in fields like biomedicine, high energy manufacturing and material chemistry.

The invention belongs to the field of medical detection, and particularly relates to a circulating tumor cell detection method based on SERS (Surface-enhanced Raman Scattering). The circulating tumorcell detection method based on SERS disclosed by the invention comprises the following steps of a, putting a solution containing circulating tumor cells on a metal nano-film substrate; b, detecting the SERS by a confocal Raman spectrometer; c, building a cell SERS discrimination model; d, distinguishing the collected Raman spectrum cell variety by the built discrimination model. According to the method, the SERS with strong signals and succinct spectral peak is used for tumor cell authentication. The method can be used for circulating tumor cell authentication, cancer early screening, therapeutic effect evaluation on cancer patients, anti-tumor medicine development and the like, and belongs to a novel technological means for clinical cancer auxiliary diagnosis. The method has the advantages that the tumor cells are not marked or damaged; the sensitivity is high; the repeated detection can be realized, and the like.

The invention discloses a Raman spectrum-based method for quickly detecting a umami substance inosine monophosphate in fresh fish flesh, relating to the technical field of aquatic product quality and safety detection. The method comprises the steps of 1, measuring a Raman spectrum of a inosine monophosphate standard substance; 2, acquiring a Raman spectrum of a fresh fish flesh sample; 3, measuring inosine monophosphate content by a chemical method; 4, performing Raman spectrum correction; 5, optimizing an inosine monophosphate characteristic peak; 6, calculating the area of the characteristic Raman peak; 7, building a detection model of the inosine monophosphate content. The umami substance inosine monophosphate in the fresh fish flesh is quickly detected by a microscopic confocal Raman spectra technique, and the Raman spectrum-based method for quickly detecting the umami substance inosine monophosphate in the fresh fish flesh which is effective, simple in operation and less in cost and time consumption is found; the detection method is simple, safe and efficient, can meet the industrialization production need and the requirements of consumers on product quality, and satisfies the need of evaluating the flavour and quality of fresh fish flesh products in a practical production and processing process.

The invention relates to a femtosecond laser machining parameter differential confocal Raman spectroscopy in-situ monitoring method and device, and belongs to the fields of a laser precision detectiontechnology and a femtosecond laser machining and manufacturing technology. A laser differential confocal axial monitoring module with high axial resolution and a femtosecond laser machining system are organically fused, and the axial position of a sample is subjected to nano-scale monitoring and sample axial machining size measurement by utilizing a curve zero point of a differential confocal system, so that the real-time focus fixing of the axial position of the sample and the high-precision measurement of the size of a micro-nano structure after machining are realized, and the drift problemand the high-precision online detection problem in the measurement process are solved; and a differential confocal Raman spectroscopy detection module is used for carrying out monitoring analysis oninformation such as the molecular structure and the like of a sample material after femtosecond laser machining, and the information is fused through a computer, so that the high-precision femtosecondlaser machining of a micro-structure and the in-situ monitoring analysis of the morphology performance of a micro-region are integrated, and the controllability of the femtosecond laser machining precision of the micro-structure, the machining quality of the sample and the like are improved.

The invention relates to a test method and device for a rear beam splitting pupil laser differential confocal Brillouin Raman spectrum, and belongs to the technical field of microscopic spectral imaging. Constructing a beam splitting pupil differential confocal microscopic imaging system through the Rayleigh scattered light which is abandoned in the confocal Raman spectrum detection system, and realizing the high spatial resolution detection of the sample geometry; obtaining the various basic properties of the detected sample by detecting the abandoned Brillouin scattered light in the confocalRaman spectrum detection system, so that measuring parameters such as elasticity, density, and elasticity of the material; acquiring the spectral information at the focal point of the sample accurately by using the focal position obtained by the beam splitting pupil laser differential confocal microscopic imaging system, so that realizing the optical splitting pupil differential confocal Brillouin-Raman spectrum high spatial resolution imaging and detection of an atlas; realizing the multi-parameter comprehensive measurement of the morphology performance of the sample by fusing the confocal Raman spectrum detection technology with the confocal Brillouin spectrum detection technology.

The invention discloses a laser confocal/differential confocal Raman spectrum vibration parameter measurement method, and belongs to the technical field of optical precision measurement. Confocal vibration detection, differential confocal vibration detection and Raman spectrum detection technologies are fused, nondestructive separation is carried out on Rayleigh light and Raman scattered light byutilizing a dichroic light splitting system, spectrum detection is carried out on the Raman scattered light, and vibration parameter detection such as amplitude and frequency is carried out on the Rayleigh light. Therefore, the vibration information testing method capable of simultaneously detecting the vibration parameters of the sample micro-area and the Raman spectrum of the vibration of the sample micro-area is formed. According to the invention, the confocal Raman microscope and the differential confocal Raman microscope have the capability of simultaneously detecting micro-area vibrationparameter information and the Raman spectrum of micro-area vibration. The method has the advantages of high vibration measurement precision, wide amplitude measurement range, high frequency measurement bandwidth, high spectrum detection sensitivity, capability of measuring periodic motion and aperiodic motion, high environmental interference resistance and the like. Meanwhile, high-precision detection of micro-area vibration parameters and vibration spectral characteristics is carried out, and the method has a wide application prospect in the technical field of optical precision measurement.

The invention relates to a space panoramic laser differential confocal Raman spectrum imaging detection method and device, and belongs to the technical field of space optical imaging and spectral measurement. A focusing telescope technology, a differential confocal technology, an image obtaining technology and a panoramic scanning technology are introduced into the spectral detection; a dichroic beam splitting system is used; Rayleigh scattering light and Raman scattering light are subjected to nondestructive separation; the characteristic that the detector differential confocal response curvezero crossing point and focal position are precisely corresponded is utilized; the telescope system is precisely controlled to automatically regulate the focus by finding the response zero crossing point, so that the laser light beam is automatically focused to a tested object; the device realizes space automatic focusing spectrum detection and image obtaining; through the panoramic scanning technology, the full circumferential space spectrum detection is realized; the method and device capable of realizing the sample space automatic focusing spectrum and image detection are formed. The method and the device have the advantages that the automatic focusing is realized; the positioning is accurate; the large-space range is realized; the full panoramic field is realized; the spectrum detection sensitivity is high; the target image is obtained, and the like.