Microscopy confocal Raman reflector path device with confocal area capable of being precisely adjusted

Abstract

The invention provides a microscopy confocal Raman reflector path device with a confocal area capable of being precisely adjusted, and belongs to the technical field of Raman spectrum. According to the microscopy confocal Raman spectrometer reflector path device, the size of the confocal area can be precisely and continuously adjusted, a microscope objective of the microscopy confocal Raman reflector path device is a zoom microscope objective, a corresponding confocal area is observed through an ocular lens, and the zoom microscope objective is adjusted to achieve visual precise and continuous adjustment of the confocal area. A light path sub system is observed in a microscopy confocal mode, a confocal area range of a microscopy confocal Raman scattering light collecting light path sub system can be accurately observed, and the zoom microscope objective is used for achieving continuous adjustment of the size of the confocal area of the microscopy confocal Raman scattering light collecting light path sub system.

Description

technical field [0001] The invention relates to the technical field of Raman spectroscopy, in particular to a micro-confocal Raman external optical path device that can precisely adjust the confocal area. Background technique [0002] Laser Raman spectroscopy is a spectral analysis technology developed based on the Raman scattering effect, which can obtain molecular vibration and rotation information of substances. Each substance molecule has its own specific characteristic Raman spectrum peak, which has been widely used in the detection of substances. identification, molecular structure studies. When the excitation light is irradiated on the substance, elastic scattering and inelastic scattering will occur. In addition to the elastic component with the same wavelength as the excitation light in the scattered light (this part of light is Rayleigh scattered light), there is also a wavelength longer than the excitation light. and short components (this part of the light is Ra...

AI Technical Summary

Problems solved by technology

Although the micro-area Raman analysis of samples of different sizes can be realized by changing the microscope objective lens with fixed magnification, there are some defects: (1) The magnification of the microscope objective lens is discontinuous, and it is impossible to continuously zoom in and observe the micro-area of ​​the sample

(3) The adjustment or replacement of the confocal pinhole is only based on personal experience and feeling. If the diameter of the confocal pinhole is too large, more Rayleigh scattering signals and stray light will enter the grating spectrometer; if the confocal pinhole is too small , a part of the weak Raman signal is blocked outside the confocal pinhole, both cases will cause a decrease in the signal-to-noise ratio

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