HOXA13 gene promoter region SNP site and application thereof to detection on intramuscular fat content of pigs

A HOXA13-F, intramuscular fat technology, applied in the field of molecular biology, can solve the problems of muscle flavor influence, muscle light reflection value and cooking loss

Active Publication Date: 2019-08-02
NANJING AGRICULTURAL UNIVERSITY
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  • Description
  • Claims
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Problems solved by technology

In addition, the intramuscular fat content will also affect the light reflection value

Method used

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  • HOXA13 gene promoter region SNP site and application thereof to detection on intramuscular fat content of pigs
  • HOXA13 gene promoter region SNP site and application thereof to detection on intramuscular fat content of pigs
  • HOXA13 gene promoter region SNP site and application thereof to detection on intramuscular fat content of pigs

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Embodiment 1

[0033] 1. Determination of intramuscular fat content of samples

[0034] 230 Pietrain×Duroc×Yorkshire×Large White quaternary hybrid commercial pigs were used. The experimental animals were provided by Wen’s Group and collected from the large-scale slaughterhouse of Su Shi Group. All experimental animals were reared under the same conditions, and the longissimus dorsi muscle was collected quickly after slaughter, and the following steps were carried out respectively:

[0035] (1) Scatter 9cm medium-speed quantitative filter paper in a clean enamel dish and bake at 105°C for 2h. Weigh with an electronic analytical balance, and record the weight of the filter paper as W1;

[0036] (2) Take about 2-3g meat samples of the longissimus dorsi muscle, chop them up, wrap them in dried filter paper and weigh them, and record the wet weight of the paper bag as W2;

[0037] (3) Spread the paper bag on an enamel plate, avoid contact, bake in an oven at 65°C for 15 hours, weigh the paper b...

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Abstract

The invention provides an SNP site for detecting the intramuscular fat content of pork. The SNP site is positioned at the upstream 1311bp position of a pig HOXA13 gene transcription initiation site; and the genotype of the SNP site is GG, GT or TT. Therefore, the SNP is named as (the formula is as shown in the description). The invention also provides a method for detecting the intramuscular fat content of the pork. The method comprises the steps of extracting the DNA of a to-be-detected pork sample, parting amplified fragments and judging the intramuscular fat content of a pork sample throughthe parting result. The method for detecting the intramuscular fat content of the pig is obtained by researching the correlation of the muscle development key gene HOXA13 and the intramuscular fat content; and based on the method, breeding of the pigs is benefited, foundation is laid for further increasing the intramuscular fat content of the pork and judging the intramuscular fat content of thepork conveniently and rapidly, and important significance in cultivating the pigs with excellent meat varieties is achieved.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a SNP site in the promoter region of the porcine HOXA13 gene and an application thereof. Background technique [0002] With the improvement of people's living standards, the quality of meat has gradually become the focus of attention. Intramuscular fat is white fat distributed in muscle tissue, and its content can change the quality of muscle in many aspects such as muscle tenderness, marbling, and muscle flavor. With the increase of intramuscular fat content, the thinner the muscle fibers, the less connective tissue between muscle fibers, the lower the shear force of the muscles, and the better the tenderness of the meat. The higher the intramuscular fat content, the higher the muscle marbling score. The higher the fat content in the muscle, the richer the lipid on the muscle surface. The film formed by the lipid on the muscle surface can relieve the degree of free...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 韦伟陈杰张立凡赵月雷林祥盛宋逸凡
Owner NANJING AGRICULTURAL UNIVERSITY
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